The role of vasoconstractile protein in renal artery and central catecholamine on renovascular hypertension

为探讨中枢儿茶酚胺是否参与两肾一夹(2K-1C)Goldblatt高血压大鼠的血压升高，进行了两系列实验。观察高血压形成前和高血压形成后脑室注射6-OHDA的效果。急性期2K-1C大鼠下丘脑去甲肾上腺素含量较假手术组低50%(p0.05)，但脑桥去甲肾上腺素含量无明显变化。2K-1C大鼠急性期下丘脑或脑桥多巴胺含量与正常血压大鼠相似，慢性期2K-1C大鼠下丘脑或脑桥多巴胺含量与假手术组相同。提示下丘脑去甲肾上腺素参与了2K-1C大鼠急性期高血压的发生发展。大鼠在夹闭一侧肾动脉前脑室注射6-OHDA，选择性消融中枢去甲肾上腺素能神经元。在2K-1C大鼠急性高血压模型上，测定了中枢儿茶酚胺和~3H-脯氨酸对血管非胶原蛋白的在体掺入，结果表明，~3H-Pro掺入血管非胶原蛋白的量增加。提示肠系膜动脉非胶原蛋白合成增加和下丘脑去甲肾上腺素浓度降低参与了2K-1C高血压的发生发展。

Two series of experiments were conducted whether the central catecholamine would participate in the elevation of blood pressure in 2-kidney, one clip (2K-1C)Goldblatt hypertensive rats. The effect of 6-OHDA injected intraventricularly before the induction of hypertension or after the establishment of hypertension was examined. The hypothalamic norepinephrine content of 2K-1C rats in acute phase is 50% lower (p 0.05) than that of sham rats, but norepicontent in pons is similar in these animals. The content of dopamine in hypothalamus,or pons is similar in 2K-1C rats and normotensive rats in acute phase.The content of these monoamines either in hypothalamus or pons in 2K-1C rats in chronic phase is identical with that in sham rats. These findings suggest that hypothalamic norepinephrine participates in the development of hypertension in 2K-1C rats in the acute phase. Rats had been intraventricular injection of 6-OHDA before clipping the unilateral renal artery that caused selective ablation of the central noradrenergic neurons. Central catecholamines and the in vivo incorporation of 3H-proline into vascular non-collagen protein were determined in 2K-1C rats in the acute hypertensive stage and results were increased incorporation into the protein fraction. It is suggested that increased non-collagen protein synthesis in the mesenteric artery and the low level of hypothalamic norepinephrine concentration partidipates in the development of 2K-1C hypertension.