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The role of vasoconstractile protein in renal artery and central catecholamine on renovascular hypertension

肾动脉血管收缩蛋白和中枢儿茶酚胺对肾血管性高血压的作用

基本信息

批准号：
63480360
负责人：
NAKADA Teruhiro
金额：
$ 1.73万
依托单位：
Department of Urology, Chiba University, School of Medicine.
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1988
资助国家：
日本
起止时间：
1988 至 1989
项目状态：
已结题

项目摘要

Two series of experiments were conducted whether the central catecholamine would participate in the elevation of blood pressure in 2-kidney, one clip (2K-1C)Goldblatt hypertensive rats. The effect of 6-OHDA injected intraventricularly before the induction of hypertension or after the establishment of hypertension was examined. The hypothalamic norepinephrine content of 2K-1C rats in acute phase is 50% lower (p 0.05) than that of sham rats, but norepicontent in pons is similar in these animals. The content of dopamine in hypothalamus,or pons is similar in 2K-1C rats and normotensive rats in acute phase.The content of these monoamines either in hypothalamus or pons in 2K-1C rats in chronic phase is identical with that in sham rats. These findings suggest that hypothalamic norepinephrine participates in the development of hypertension in 2K-1C rats in the acute phase. Rats had been intraventricular injection of 6-OHDA before clipping the unilateral renal artery that caused selective ablation of the central noradrenergic neurons. Central catecholamines and the in vivo incorporation of 3H-proline into vascular non-collagen protein were determined in 2K-1C rats in the acute hypertensive stage and results were increased incorporation into the protein fraction. It is suggested that increased non-collagen protein synthesis in the mesenteric artery and the low level of hypothalamic norepinephrine concentration partidipates in the development of 2K-1C hypertension.

本实验采用二肾一夹（2K-1C）Goldblatt高血压大鼠模型，进行了中枢儿茶酚胺是否参与血压升高的两个系列实验。在诱导高血压之前或建立高血压之后，检查脑室内注射6-OHDA的效果。2K-1C大鼠急性期下丘脑去甲肾上腺素含量比假手术组低50%（p0.05），而脑桥去甲肾上腺素含量与假手术组相似。2K-1C大鼠急性期下丘脑和脑桥多巴胺含量与正常血压大鼠相似，慢性期下丘脑和脑桥单胺类递质含量与假手术大鼠相似。这些结果表明，下丘脑去甲肾上腺素参与了2K-1C大鼠急性期高血压的发生发展。大鼠在夹闭一侧肾动脉前，脑室内注射6-OHDA，导致中枢去甲肾上腺素能神经元选择性消融。在2K-1C大鼠急性高血压阶段测定了中枢儿茶酚胺和3 H-脯氨酸掺入血管非胶原蛋白的体内掺入，结果是掺入蛋白组分增加。提示肠系膜动脉非胶原蛋白合成增加和下丘脑去甲肾上腺素浓度降低参与了2K-1C型高血压的发生。