The new Leavening Bacteria in dough of Wheat flour

据报道，野生酵母和其他微生物参与了中国的配方。（1）对发酵剂和酵母制备的馒头进行感官评定，结果表明：不同样品的感官评定结果一致，尤其是发酵剂制备的馒头口感较好，而酵母风味较差。（2）在纯分离时鉴定出几种微生物。经鉴定为阴沟肠杆菌GAO。（3）确定了E. API生物型。clobacillus GAO和ATCC 13047，发现两者均为3305573。热稳定肠毒素的API生物型为3305763，与分离的E. clopidoglobin GAO和ATCC 13047。（4）BALB品系裸鼠给予浓缩混悬液的结果显示，腹腔内注射或口服探针后，未观察到器官重量的变化。未观察到器官和腹泻等异常结果，也没有注意到。因此，一般认为，正常动物和人类的非毒性微生物感染对裸鼠是致命的。考虑到这些事实，E. Clobacillus GAO没有传染性，而且也不可能产生肠毒素样物质。根据这些事实，E。认为Clobacillus GAO及其培养基不具有感染性，并且鉴于器官重量和器官结果，未形成关于产生肠毒素样物质的意见。目前正在对这种细菌和使用这种细菌的发酵食品馒头进行慢性毒性测试。

It has been reported the wild yeasts and other microorganisms are involved in the Chinese recipe.(1)The results for the sensory test of Mantou prepared with starter and yeasts showed as same preference among samples,especially Mantou with starter have favorable taste not yeast flavor.(2)Several species of microorganisms were identified when they were purely isolated. The microorganisms was identified as Enterobacter cloacae GAO.(3)The API biotype was determined for E. cloacae GAO and ATCC 13047, both of them were found be 3305573. The API biotype of the heat-stable enterotoxin was 3305763, which was different from that of the isolated E. cloacae GAO and ATCC 13047, respectively.(4)The results upon administration of the concentrated suspension to nude mice of the BALB strain shows no changes in organ weights were observed after intraperitone injection or oral administration with a probe. No abnormal finding was observed on organs, and diarrhea, etc., were not noted either. Therefore,it is generally assumed that infections of non-virulent microorganisms for normal animals and humans are fatal for nude mice. Considering these facts, it seems likely that E. cloacae GAO has no infectivity.Moreover, the production of an enterotoxin-like substance is out of the question. From these facts, E. cloacae GAO and its culture medium were considered not to have infectivity, and in view of the organ weights and the findings on organs, an opinion as to the production of an enterotoxin-like substance was not formed. The chronic toxicity testing of this bacterium and of mantou as a fermented food using this bacteium is now in progress.