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Gibberellin production and cell differentiation in plant cell culture

植物细胞培养中的赤霉素产生和细胞分化

基本信息

批准号：
62560136
负责人：
SAKURAI Akira
金额：
$ 1.28万
依托单位：
The Institute of Physical and Chemical Research (RIKEN)
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1987
资助国家：
日本
起止时间：
1987 至 1988
项目状态：
已结题

项目摘要

The relationship between gibberellin (GA) production and morphogenesis of plant cells was investigated by analyzing GA contents in crown gall cells of different morphology using GC-SIM after purification of GA fractions from cell extracts.1. Two kinds of cells, an unorganized cell and a shoot-forming teratoma, were obtained by cloning tobacco cells having nopaline type Ti-plasmid (pTi-T37). The shoot-forming teratoma contained higher levels of C_<2 0>-GAs, such as GA_<1 9>, GA_<2 3> and GA_<5 3> (700-800 pg/gr fr wt)than that of the unorganized cells (less than 100 pg/gr fr wt).2. GA contents in nopaline type and octopine type crown gall tissues of Catharanthus roseus were compared. Biologically active GAs were only detected in the extracts of the nopaline type cells which is able to develop shoots easily. The one of GAs in this nopaline type cells was identified as GA_<2 4>, one of C_<2 0>-GAs, by GC-MS.3. Shoot-forming tobacco crown gall tissue obtained by inducing nopaline type Ti-plasmid has ent-kaurene synthesis capacity. Main GAs in this cell were found to be C20-GAs, and their levels were almost the same as that of the shoot-forming teratoma described in the above 1.From the above results, there must be a close correlation between GA production and morphogenesis of crown gall tissues. Gibberellin biosynthesis ability appears in shoot-forming cells similar to normal plant tissues, while it is supressed in unorganized plant cells.

纯化细胞提取物中的GA组分后，采用GC-SIM分析不同形态的冠瘿细胞中赤霉素(GA)的含量，探讨赤霉素(GA)产量与植物细胞形态发生的关系。 1．通过克隆具有胭脂碱型Ti质粒(pTi-T37)的烟草细胞，获得两种细胞，无组织细胞和芽形成畸胎瘤。芽形成畸胎瘤含有较高水平的C_<2 0>-GA，如GA_<1 9>、GA_<2 3>和GA_<5 3>(700-800 pg/gr wt)，高于无组织细胞(低于100 pg/gr wt)。2．比较胭脂碱型和章鱼碱型长春花冠瘿组织中GA的含量。仅在胭脂碱型细胞的提取物中检测到具有生物活性的GA，该细胞能够容易地长出枝条。通过GC-MS，将该胭脂碱型细胞中的GA之一鉴定为GA_ 2 4 、C_ 2 0 -GA之一。3。诱导胭脂碱型Ti质粒获得的烟草冠瘿组织具有对映贝壳杉烯合成能力。发现该细胞中的主要GA是C20-GA，其水平与上述1中描述的芽形成畸胎瘤的水平几乎相同。从上述结果来看，GA的产生与冠瘿组织的形态发生之间必然存在密切的相关性。赤霉素的生物合成能力出现在类似于正常植物组织的芽形成细胞中，而在无组织的植物细胞中则受到抑制。