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Effects of Prolactin on Pancreatic Function in Mice

催乳素对小鼠胰腺功能的影响

基本信息

批准号：
63540596
负责人：
MORI Takao
金额：
$ 1.28万
依托单位：
University of Tokyo
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1988
资助国家：
日本
起止时间：
1988 至 1989
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-63540596/
关键词：
mouse prolactin hyperprolactinemia pancreas insulin hyperinsulinemia hyperglycemia 高血糖

项目摘要

Prolactin treatment directly stimulates insulin release from B-cells of rat pancreatic islets in vitro and in vivo, whereas insulin is shown to stimulate prolactin secretion from uterine decidual cells and pituitary tumor cells in vitro. These findings indicate an intimate relationship between prolactin and insulin secretion. Thus, it is worthwhile examining the long-term effects of prolactin on pancreas in an animal model.Inbred strains of BALB/c and SHN mice were used in these experiments. Male and female mice of these strains were grafted with anterior pituitaries single each at the pancreata at 50 days of age and used at various intervals after the transplantation. Control mice of these strains were given a piece of submaxillary gland each at the same site. All grafts were obtained from the 50-day-old male mice of the respective strains.Ectopic pituitary transplantation resulted in an elevation of plasma prolactin level, hyperprolactinemia, and an increase in the pancreatic weight, mainly due to hyperplastic proliferation of the pancreatic acinar glands. In addition, blood glucose levels in the mice with pituitary grafts were higher than in mice without pituitary grafts. On the other hand, serum insulin levels in mice with pituitary grafting were significantly higher than in mice without pituitary grafts. Pituitary grafting also induced an significant enlargement of the liver weight.It is widely recognized that any conditions leading to hyperglycemia are highly related to pancreatic tumorigenesis. The present results imply that hyperprolactinemia associated with hyperglycemia is one of the causes of pancreatic tumorigenesis. In conclusion, the high circulating level of prolactin induced by the ectopic pituitary grafting plays an important role in the development of pancreatic hyperplasia.

催乳素治疗在体外和体内直接刺激大鼠胰岛B细胞释放胰岛素，而胰岛素在体外刺激子宫蜕膜细胞和垂体瘤细胞分泌催乳素。这些发现表明催乳素和胰岛素分泌之间存在密切关系。因此，在动物模型中研究催乳素对胰腺的长期影响是值得的。在这些实验中使用近交系的BALB/c和SHN小鼠。这些品系的雄性和雌性小鼠在50日龄时在胰腺处各移植一个前垂体，并在移植后的不同时间间隔使用。这些品系的对照小鼠在相同部位各给予一片颌下腺。所有移植物均取自50日龄的各品系雄性小鼠，异位垂体移植导致血浆催乳素水平升高、高催乳素血症和胰腺重量增加，主要是由于胰腺腺泡腺增生所致。此外，有垂体移植物的小鼠的血糖水平高于没有垂体移植物的小鼠。另一方面，垂体移植小鼠的血清胰岛素水平显著高于未移植垂体的小鼠。胰腺移植后肝脏重量明显增加，任何导致高血糖的情况都与胰腺肿瘤的发生密切相关。提示高催乳素血症伴高血糖是胰腺肿瘤发生的原因之一。结论：异位垂体移植引起的高泌乳素循环水平在胰腺增生的发生发展中起重要作用。