Studies on the evolutim of hormare receptons.

激素受体进化的研究。

• 批准号: 06454021
• 负责人: MORI Takao
• 金额: $ 4.54万
• 依托单位: Graduate School of Sciance, University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06454021/
• 关键词: Hormone receptor; genome; vertbenate; reproduction; evolution

For understanding the evolutionary ralationships between gonadotropins (LH and FSH) and their receptors, we attempted to charatcterized the extracellulara domain of the receptors, which is thought to be a key region of hormone binding, in nonmammalian species, and to compare the information to that of the known mammalian data. By using polymerase chain reaction (PCR) and reverse transcription (RT)-PCR,parts of the quail and newt FSH receptor cDNAs were amplified. The homology of predicted peptide of the quail and newt FSH receptors was about 70% and 67% of those of mammalian FSH receptors, resepectively. We also charactreized cDNA of the quail LH receptor. The homology of both cDNA and predicted peptide was about 70% of those of mammalian LH receptors. These data suggetsts that the ligand binding domain of receptro is more conserved than the ligand molecules during their evolution. To reinforce this hypothesis, molecular characterization of FSH and LH receptors in a wider range of vertebrate classes is needed.

为了了解促性腺激素（LH和FSH）与其受体之间的进化关系，我们试图对非哺乳动物中被认为是激素结合的关键区域的受体胞外区进行表征，并与已知的哺乳动物数据进行比较。采用聚合酶链反应（PCR）和逆转录（RT）-PCR技术，扩增了鹌鹑和蝾螈FSH受体的部分cDNA。预测的鹌鹑和蝾螈FSH受体肽段与哺乳动物FSH受体肽段的同源性分别为70%和67%。我们还鉴定了鹌鹑LH受体的cDNA。其cDNA和预测肽的同源性约为哺乳动物LH受体的70%。这些数据表明，受体的配体结合结构域在其进化过程中比配体分子更保守。为了加强这一假设，FSH和LH受体在更广泛的脊椎动物类的分子特征是必要的。

项目成果

M.K.Park: "Characteriz ation of gonadotiopin-relearing hormme(GnKH)immunoreactive psalein in the sst pineal gland" Journal of Neuroscience Research. 41. 386-393 (1995)

M.K.Park：“sst 松果体中促性腺激素释放激素 (GnKH) 免疫反应性 psalein 的特征”神经科学研究杂志。

Matsuda,M: "Enhanced cell praliferation by hyperpralactinemia in both exocrine and endocrine pancreas in mice" Europ.J.Endocrinol.130. 187-194 (1994)

Matsuda,M：“小鼠外分泌和内分泌胰腺中高普拉乳素血症增强细胞增殖”Europ.J.Endocrinol.130。

H.Kogo: "In situ detection of gonadotiopin-releasing hormone (GnRH) recepton mRNA expression in the rat ovarion pollioles" J. Experimental Zoology. 272. 62-68 (1995)

H.Kogo：“大鼠卵巢小球中促性腺激素释放激素 (GnRH) 受体 mRNA 表达的原位检测”J. 实验动物学。

K.Kubokawa et al: "Effect of day length on luteimizing hormone beta-sabumit mRNA and subsequent gonadal porath in the wbitecrouned sparrom, Zonotriclia kucophrys jamabli" General and Comparative Endoainology. 95. 42-51 (1995)

K.Kubokawa 等人：“白昼长度对黄体生成素 β-sabumit mRNA 和随后的 wbitecrouned sparrom（Zonotriclia kucophrys jamabli）性腺孔的影响”普通和比较内科。

M.Ashihara: "Two differing precurson genes fr the salmon type gonadotrepin-roleasing hormne exist in salmonids" Journal of Molecular Endocrinology. 15. 1-9 (1995)

M.Ashihara：“鲑鱼中存在两种不同的鲑鱼型促性腺激素释放激素的前体基因”《分子内分泌学杂志》。