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Mechanisms of proliferation of uterus in mice

小鼠子宫增殖机制

基本信息

批准号：
02804067
负责人：
MORI Takao
金额：
$ 1.09万
依托单位：
University of Tokyo
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1990
资助国家：
日本
起止时间：
1990 至 1991
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-02804067/
关键词：
mouse uterus prolactin growth factors estrogen enzymes プロテクチン細胞培養

项目摘要

Uterus is the most important organ for the mammalian reproduction. Proliferation of the uterus is under the control of estrogen and progesterone. Recently it is known that action of estrogenis not direct, but indirect. Estrogen may produce some kinds of growth factors which directly affect the proliferation of uterus.In this research project, I had designed to find novel growth factors which would control the proliferation of the uterine tissues. In our previous works, I have found that in mice anterior pituiary isogragfting into uterine lumen induces an early and high incidence of uterine adenomysis, a benign pathological disorder of the endometrial tissues, glands and stroma within the myometrium. Thus, I had used this animal model to find new growth factors in the uterus showing a marked proliferation of the tissues. However, I could not detect noel growth factors when the uterine tissues and fluid were analyzed by electrophoresis. The, I had examined the activities of both thymidylate synthetase and thymidine kinase, i. e., DNA-synthesizing enzymes. The results showed that both enzyme activities were significantly increased in the uterus with adenomyosis to two-fold those in the normal control uterus. This findings strongly suggest that the animal model developing uterine adenomyosis is useful for the study of the mechanisms of uterine proliferation. Thus, I would like to continue this research project by using the animal model of uterine adenomyosis in mice.

子宫是哺乳动物最重要的生殖器官。子宫的增生是在雌激素和孕激素的控制下进行的。近年来发现雌激素的作用不是直接的，而是间接的。雌激素可能产生一些直接影响子宫增殖的生长因子，本研究旨在寻找新的抑制子宫组织增殖的生长因子。在我们以前的工作中，我已经发现，在小鼠中，前垂体同向移植到子宫腔中诱导子宫腺肌病的早期和高发病率，子宫内膜组织，腺体和子宫肌层内的基质的良性病理性疾病。因此，我用这个动物模型在子宫中发现了新的生长因子，显示出组织的显著增殖。然而，当我对子宫组织和子宫液进行电泳分析时，我不能检测到诺埃尔生长因子。此外，我还检测了胸苷酸合成酶和胸苷激酶的活性。即，DNA合成酶结果显示，子宫腺肌病患者子宫内两种酶活性均明显升高，是正常对照组的2倍。这一结果有力地表明，子宫腺肌症的动物模型是有用的子宫增生的机制研究。因此，我想通过使用小鼠子宫腺肌病的动物模型来继续这个研究项目。