MECHANISMS OF INTERACTION OF TIN AND OTHER ELEMENTS IN MICE AND IDENTIFICATION OF TIN-BINDING PROTEIN IN ORGANS

研究了无机锡（SnCl_2）和有机锡（TBTC）对小鼠的影响。注射SnCl_2后，观察到以下结果：Sn在骨、脾、肝、胰、肾中大量检出，在脑中未检出。当Na_2SeO_3与SnCl_2同时注入时，这种分布模式发生了改变；即脾脏和胰腺中Sn含量升高，骨骼和肾脏中Sn含量降低，肝脏中Sn含量无变化，全血中2.90%的Sn在红细胞中，Sn抑制了红细胞中δ -氨基乙酰丙酸脱水酶（ALAD）的活性，3。红细胞中有4种锡结合蛋白，分别为巨球蛋白、ALAD、Hb和50kd蛋白。硒与SnCl_2的结合阻止了Sn进入ALAD，使ALAD活性保持在正常水平，肝脏中4.10%的Sn存在于细胞质中，有5种锡结合蛋白存在于细胞质中。Sn对肝细胞质ALAD活性无抑制作用。注射TBTC后，观察如下结果：1。全血中有2.80%的锡存在于红细胞中，而红细胞中ALAD活性不受影响。综上所述，有机锡和无机锡对小鼠各器官分布的影响不同，主要在脑和骨。SnCl_2对红细胞ALAD活性有抑制作用，而对肝细胞质无抑制作用。硒和锡对SnCl_2组小鼠的生物学效应的影响大于TBTC组小鼠。

The effects of tin, inorganic(SnCl_2) and organic(tributyltin chloride, TBTC), were investigated in mice. After injection of SnCl_2, the following results were observed: 1. Sn was largely detected in bone, spleen, liver, pancreas, and kidney, and was not detected in brain. When Na_2SeO_3 was injected together with SnCl_2, this distribution pattern was modified; namely Sn contents increased in spleen and pancreas, decreased in bone and kidney, and no change in liver, 2.90% of Sn in whole blood was in RBC, and Sn inhibited the activity of delta-aminolevulinic acid dehydratase(ALAD) in RBC, 3. there were 4 tin-binding proteins in RBC, corresponding to macroglobulin, ALAD, Hb, and 50 KD protein. Se accompanied with SnCl_2, prevented Sn from entering ALAD, therefore ALAD activity was kept at a normal level, 4.10% of Sn in liver was in cytosol fraction, and 5 tin-binding proteins were in liver cytosol. Sn did not inhibit ALAD activity in liver cytosol. After injection of TBTC, the following results were observed: 1. Sn was detected in every organ including brain, however, little Sn was found in bone, 2.80% of Sn in whole blood was in RBC, however, ALAD activity in RCB was not influenced.In summary, the effects of organic and inorganic tin on distribution in various organs in mice were different, mainly in brain and bone. SnCl_2 inhibited ALAD activity in RBC, but not in liver cytosol. The modification by Se accompanied with Sn on the biological effects was observed in mice administered SnCl_2 rather than in mice administered TBTC.