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MECHANISMS OF INTERACTION OF TIN AND OTHER ELEMENTS IN MICE AND IDENTIFICATION OF TIN-BINDING PROTEIN IN ORGANS

小鼠体内锡与其他元素相互作用的机制及器官中锡结合蛋白的鉴定

基本信息

批准号：
63570242
负责人：
CHIBA Momoko
金额：
$ 1.22万
依托单位：
Juntendo University School of Medicine
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1988
资助国家：
日本
起止时间：
1988 至 1989
项目状态：
已结题

项目摘要

The effects of tin, inorganic(SnCl_2) and organic(tributyltin chloride, TBTC), were investigated in mice. After injection of SnCl_2, the following results were observed: 1. Sn was largely detected in bone, spleen, liver, pancreas, and kidney, and was not detected in brain. When Na_2SeO_3 was injected together with SnCl_2, this distribution pattern was modified; namely Sn contents increased in spleen and pancreas, decreased in bone and kidney, and no change in liver, 2.90% of Sn in whole blood was in RBC, and Sn inhibited the activity of delta-aminolevulinic acid dehydratase(ALAD) in RBC, 3. there were 4 tin-binding proteins in RBC, corresponding to macroglobulin, ALAD, Hb, and 50 KD protein. Se accompanied with SnCl_2, prevented Sn from entering ALAD, therefore ALAD activity was kept at a normal level, 4.10% of Sn in liver was in cytosol fraction, and 5 tin-binding proteins were in liver cytosol. Sn did not inhibit ALAD activity in liver cytosol. After injection of TBTC, the following results were observed: 1. Sn was detected in every organ including brain, however, little Sn was found in bone, 2.80% of Sn in whole blood was in RBC, however, ALAD activity in RCB was not influenced.In summary, the effects of organic and inorganic tin on distribution in various organs in mice were different, mainly in brain and bone. SnCl_2 inhibited ALAD activity in RBC, but not in liver cytosol. The modification by Se accompanied with Sn on the biological effects was observed in mice administered SnCl_2 rather than in mice administered TBTC.

本文研究了无机锡（SnCl_2）和有机锡（氯化三丁基锡，TBTC）对小鼠的影响。注入SnCl_2后，观察到以下结果：1. Sn主要在骨、脾、肝、胰和肾中检测到，而在脑中未检测到。当Na_2SeO_3与SnCl_2同时注射时，这种分布模式发生了改变，即脾脏和胰腺中的Sn含量增加，骨骼和肾脏中的Sn含量减少，肝脏中的Sn含量无变化，全血中2.90%的Sn存在于红细胞中，Sn抑制红细胞中的δ-氨基乙酰丙酸脱氢酶（ALAD）活性。红细胞中有4种锡结合蛋白，分别对应于巨球蛋白、ALAD、Hb和50 KD蛋白。硒与SnCl_2共存，阻止了锡进入ALAD，使ALAD活性保持在正常水平，肝脏中4.10%的锡存在于胞液中，5种锡结合蛋白存在于胞液中。锡没有抑制ALAD活性在肝细胞质。注射TBTC后，观察到以下结果：1.结果表明，有机锡和无机锡对小鼠各器官的分布影响不同，主要分布在脑和骨中，全血中2.80%的锡存在于红细胞中，但对红细胞ALAD活性无影响。SnCl_2对红细胞ALAD活性有抑制作用，但对肝胞液ALAD活性无抑制作用。硒与锡共存时，对SnCl_2组的生物学效应的影响大于TBTC组。