Health problem due to magnesium and/or potassium deficiency-Experimental study using stable isotope-

日本等亚洲国家也发生过因过度工作或压力过大而导致的猝死事件。许多病例是年轻男性。我们估计，这一假设将是慢性矿物质平衡紊乱以及压力。镁（Mg）、钾(K)缺乏症引起重视。在给予缺镁和/或缺钾饮食的雄性和雌性小鼠中观察到死亡率。(1)单纯缺镁小鼠无性别差异；(2)幼龄缺镁小鼠2周内雄鼠死亡，雌鼠全部存活；4月龄小鼠1周后死亡率曲线移位；(3)阉割缺镁小鼠死亡率无性别差异。正常情况下，缺镁小鼠各器官中Mg浓度极低，但心脏和肾脏中Mg浓度维持在正常范围。静脉注射稳定同位素26Mg后，在心脏和肾脏中发现了相当数量的26Mg。心脏26Mg升高至6小时。在正常条件下（23℃，60%）和高温条件下（30℃，60%），并给予正常或缺镁饮食的小鼠，观察脂质过氧化和矿物质平衡。在高温条件下饲养的小鼠脂质过氧化增加，与饮食无关。缺乏镁钾饮食和高温环境下居住的雄性小鼠出现了矿物质平衡紊乱。综上所述，年轻雄性小鼠易受Mg和/或K缺乏的影响，体内平衡受到损害。

Sudden death cases, which were considered due to over work or stress, were happened in Asian countries including Japan. Many cases were young males. We have estimated that the hypothesis would be chronic disorder of mineral balance as well as stress. Deficiency of magnesium (Mg) and potassium (K) was paid attention.1. Mortality rates were observed in male and female mice given Mg and/or K deficient diet. (1) no sexual difference was observed in mice given only Mg deficient diet, (2) in young mice given Mg/K deficient diet, males were died within 2 weeks, while all females were survived, and in 4 month old mice, the mortality curves were shifted 1 week later, (3) in mice received castration and given Mg/K deficient diet, no sexual difference was observed in mortality rate.2. As the natural results, Mg concentrations were extremely low in various organs of mice fed Mg deficient diet, however, Mg concentration in heart and kidney were kept at the normal ranges.3. After intravenous injection with stable isotope 26Mg, considerable amount of 26Mg was found in heart and kidney. In heart 26Mg was increasing up to 6 hours.4. Lipid peroxide and mineral balance were observed in mice housing in the normal conditions (23 C, 60%) or in hot conditions (30 C, 60%) and given normal or Mg/K deficient diet. Lipid peroxide increased in mice housing in hot conditions, and independent from diet. Disorder of mineral balance was appeared in male mice fed Mg/K deficient diet and housing in hot conditions.As conclusion, young male mice were susceptible for Mg and/or K deficiency, and their homeostasis was injured.