Health problem due to magnesium and/or potassium deficiency-Experimental study using stable isotope-

包括日本在内的亚洲国家都发生了被认为是由于过度工作或压力造成的猝死病例。许多病例为年轻男性。我们估计，这一假说将是慢性疾病的矿物质平衡以及压力。缺镁、缺钾是引起重视的问题.在给予镁和/或钾缺乏饮食的雄性和雌性小鼠中观察到死亡率。(1)（2）幼龄小鼠缺镁/缺钾饮食组雄性小鼠2周内死亡，雌性小鼠全部存活，4月龄小鼠死亡率曲线在1周后发生偏移;（3）去势小鼠缺镁/缺钾饮食组死亡率无性别差异.缺镁饲料喂养的小鼠各器官中镁含量极低，心脏和肾脏中镁含量维持在正常范围，这是自然的结果.静脉注射稳定性同位素26 Mg后，心脏和肾脏中有相当数量的26 Mg。在心脏中26 Mg增加到6小时。在正常条件（23 ℃，60%）或热条件（30 ℃，60%）下饲养并给予正常或Mg/K缺乏饮食的小鼠中观察脂质过氧化物和矿物质平衡。脂质过氧化物增加小鼠住房在炎热的条件下，独立于饮食。低镁、低钾饮食和高温条件下饲养的雄性小鼠出现矿物质平衡紊乱，说明幼年雄性小鼠易患镁和/或钾缺乏，体内平衡受损。

Sudden death cases, which were considered due to over work or stress, were happened in Asian countries including Japan. Many cases were young males. We have estimated that the hypothesis would be chronic disorder of mineral balance as well as stress. Deficiency of magnesium (Mg) and potassium (K) was paid attention.1. Mortality rates were observed in male and female mice given Mg and/or K deficient diet. (1) no sexual difference was observed in mice given only Mg deficient diet, (2) in young mice given Mg/K deficient diet, males were died within 2 weeks, while all females were survived, and in 4 month old mice, the mortality curves were shifted 1 week later, (3) in mice received castration and given Mg/K deficient diet, no sexual difference was observed in mortality rate.2. As the natural results, Mg concentrations were extremely low in various organs of mice fed Mg deficient diet, however, Mg concentration in heart and kidney were kept at the normal ranges.3. After intravenous injection with stable isotope 26Mg, considerable amount of 26Mg was found in heart and kidney. In heart 26Mg was increasing up to 6 hours.4. Lipid peroxide and mineral balance were observed in mice housing in the normal conditions (23 C, 60%) or in hot conditions (30 C, 60%) and given normal or Mg/K deficient diet. Lipid peroxide increased in mice housing in hot conditions, and independent from diet. Disorder of mineral balance was appeared in male mice fed Mg/K deficient diet and housing in hot conditions.As conclusion, young male mice were susceptible for Mg and/or K deficiency, and their homeostasis was injured.