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Experimental Study on the Effect of Stress on the Upper Respiratory Tract

应激对上呼吸道影响的实验研究

基本信息

批准号：
63570815
负责人：
TACHIBANA Masayoshi
金额：
$ 1.28万
依托单位：
Meiji College of Oriental Medicine
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1988
资助国家：
日本
起止时间：
1988 至 1989
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-63570815/
关键词：
stress electroshock moxibustion rat nasal mucosa goblet cell #細胞

项目摘要

In order to investigate the effect of stress on the upper respiratory tract, the activity of goblet cell of the rat nasal mucosa under two kinds of stress was examined by the histochemical technique.Male Wistar rats weighing ca. 2OOg was dived into three groups. First group was put into stress cage and direct current of 15 volts was administrated from the tail intermittently (5 seconds with 30 seconds interval) for 60 min. Second group was stimulated by moxibustion at apex of the head 5 times under pentobarbital anesthesia. Third group was the control without any stimulation.After the decapitation under anesthesia the nasal mucosa was dissected and fixed by buffered 10 % formalin. The tissue was then stained with alcian-blue(PH 2.5) and the epithelium at the area between 6 and 8 mm from the tip was further dissected and mounted on the slide glass and observed under light microscope at x 400. The stained area was estimated by means of computer-aided image analyzer.The staining was shown to be due to secretory granules of the goblet cell by observing the thin sections of the Epon-embedded stained specimen. Thus the percentage of the stained area can be understood to reflect the secretory function of the nasal mucosa. The function was significantly larger in the electrically shocked rats and smaller in rats stimulated by moxibustion.From these results, it is now evident that stress does influence the secretory activity of upper respiratory tract. And interestingly, the direction of the influence depends on the kind of stress; uncomfortable stress increased the secretory activity of goblet cell of the rat nasal mucosa and comfortable stress caused the opposite effect. Therefore, comfortable stress such as moxibustion may be useful to counteract or minimize the noxious effect of umconfortable stress in the upper respiratory tract.

为探讨应激对上呼吸道的影响，用组织化学方法观察了两种应激状态下大鼠鼻粘膜杯状细胞的活性变化。200 g，分为三组。第一组置于应激笼中，从尾部间断（5 s，30 s为间隔）给予15 V直流电60 min;第二组在戊巴比妥钠麻醉下，在头顶部艾灸5次。第三组为对照组，麻醉下断头取鼻粘膜，10%福尔马林固定。然后用阿尔西安蓝（PH 2.5）对组织进行染色，并进一步解剖距头端6 - 8 mm区域的上皮，固定在载玻片上，在光学显微镜下以x 400观察。用计算机图像分析仪计算染色面积，通过Epon包埋染色标本的薄片观察，证明染色是杯状细胞分泌颗粒所致。因此，染色面积的百分比可以理解为反映鼻粘膜的分泌功能。结果表明，应激对上呼吸道分泌活动有影响。刺激对大鼠鼻黏膜杯状细胞分泌的影响方向与刺激的种类有关，不舒适刺激可使杯状细胞分泌活性增加，而舒适刺激则相反。因此，舒适的压力，如艾灸，可能是有用的，以抵消或尽量减少不舒适的压力在上呼吸道的有害影响。