Immunocytochemical Studies on the Infection and Multiplication of Viruses in Plant Cells by Protein A-gold Technique.
利用 Protein A-gold 技术对植物细胞中病毒的感染和增殖进行免疫细胞化学研究。
基本信息
- 批准号:01560048
- 负责人:
- 金额:$ 1.34万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for General Scientific Research (C)
- 财政年份:1989
- 资助国家:日本
- 起止时间:1989 至 1990
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Immunocytochemical localization of structural and non-structural proteins of tobacco mosaic virus (TMV) in tobacco protoplasts and brome mosaic virus (BMV) in barley cells were investigated by protein A-gold technique.TMV : When utilizing the anti-coat protein antiserum, gold labels were first found in small areas in the cytoplasm 4 hr after inoculation and followed immediately by occurrence of virus particles. Subsequently, virus particles formed crystalline arrays and during the period of active virus multiplication, labels were confined only on this virus crystalline aggregates. In later stages of infection, lighter labels also were found throughout the cytoplasm where no virus particles were evident. Nucleus, plastid and mitochondria were free of labels throughout infection. Labels with antiー130K protein antiserum were first detected on virusーinduced small inclusions in the cytoplasm 4 hr after inoculation. Several inclusions in a protoplast occurred, and they are often in the vici … More nity of virus aggregates. The rapid increasing in the size of the inclusion were take placed during later stages of infectin, in which virus replication are slowdown, rather than during vigorous production of virus. Labels were always associated with this inclusions. The labels with antiー180K protein antiserum were also dispersed over this inclusions. When sections were treated with antiー30K protein antiserum, specific gold labels were first observed at several small regions in the cytoplasm at 4-6 hr after inoculation. Thereafter, the number of labeling areas increased along the time course. When protoplasts were cultured in the presence of actinomycin D (30mug/ml). Large areas in the cytoplasm were heavily labeled. These labeling areas were slightly less electronーdense than surrounding cytoplasm and fulled of granular structures.BMV : When the coat protein antiserum were used, much gold labels were observed through out the cytoplasm of barley cells. In particular, heavy labels were found on the region of high concentration of virus particles in the cytoplasm. Specific but less dense labeling was observed over some nuclei infected cells. However, specific labeling was not present inside chloroplast, mitochondria and microbodies. Gold labels of 3a protein were found over the region where very high concentrations of vurus particles occur in the cytoplasm. Less
应用蛋白A-金标记技术,对烟草花叶病毒(TMV)和雀麦花叶病毒(BMV)的结构蛋白和非结构蛋白在烟草原生质体和大麦细胞中的定位进行了研究。随后,病毒颗粒形成晶体阵列,并且在活跃的病毒增殖期间,标记仅被限制在该病毒晶体聚集体上。在感染的后期,在整个细胞质中也发现了较轻的标记,其中没有明显的病毒颗粒。细胞核、质体和线粒体在整个感染过程中均无标记。在接种后4小时,首先在细胞质中的病毒囊泡诱导的小包涵体上检测到具有抗E130 K蛋白抗血清的标记。在原生质体中存在多个内含物,它们通常位于维西附近 ...更多信息 病毒聚集体的数量。包涵体大小的迅速增加发生在病毒复制减慢的感染后期,而不是病毒的旺盛生产期。标签总是与这种夹杂物相关联。用抗HPLC 180 K蛋白抗血清的标记物也分散在该包涵体上。当切片用抗HSP 30 K蛋白抗血清处理时,在接种后4-6小时,在细胞质中的几个小区域首先观察到特异性金标记。此后,标记区域的数量沿着时间进程增加。当原生质体在放线菌素D(30 μ g/ml)存在下培养时。细胞质中的大区域被大量标记。BMV:用外壳蛋白抗血清标记大麦细胞时,在整个细胞质中观察到大量的金标记。特别地,在细胞质中病毒颗粒的高浓度区域上发现重标记。在一些细胞核感染的细胞上观察到特异性但不太密集的标记。然而,在叶绿体、线粒体和微体中不存在特异性标记。3a蛋白的金标记物被发现覆盖在细胞质中出现非常高浓度的vurus颗粒的区域。少
项目成果
期刊论文数量(7)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
細川 大二郎: "タバコモザイクウイルス3aタンパク貭のプロトプラストにおける局在" 平成3年度日本植物病理学会大会発表予定.
Daijiro Hosokawa:“烟草花叶病毒 3a 蛋白在原生质体中的定位” 计划在 1991 年日本植物病理学会大会上发表。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Meshi,T.: "Intracellular localization of the 30K protein in tobacco protoplasts infected with tobacco mosaic virus RNA" Virology.
Meshi,T.:“感染烟草花叶病毒 RNA 的烟草原生质体中 30K 蛋白的细胞内定位”病毒学。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
細川 大二郎: "ブロムモアザイクウイルスの3aおよび外被タンパク質のオオムギ感染細胞における局在" 日本植物病理学会報. 56. 418 (1990)
Daijiro Hosokawa:“大麦感染细胞中溴泽病毒 3a 和外壳蛋白的定位”日本植物病理学会通报 56. 418 (1990)。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
細川 大二郎: "タバコモザイクウイルス 3a タンパク質のプロトプラストにおける局在" 平成3年度 日本植物病理学会大会発表予定.
Daijiro Hosokawa:“烟草花叶病毒 3a 蛋白在原生质体中的定位” 计划在 1991 年日本植物病理学会大会上发表。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
細川 大二郎: "ブロムモザイクウイルスの3aおよび外被タンパク貭のオオムギ感染細胞における局在" 日本植物病理学会報. 56. 418 (1990)
Daijiro Hosokawa:“大麦感染细胞中雀麦花叶病毒 3a 和外壳蛋白的定位”日本植物病理学会通报 56. 418 (1990)。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
HOSOKAWA Daijiro其他文献
HOSOKAWA Daijiro的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('HOSOKAWA Daijiro', 18)}}的其他基金
Studies on the multiplication mechanism of plant viruses by electron microscopic in situ hybridization
电镜原位杂交研究植物病毒增殖机制
- 批准号:
07660054 - 财政年份:1995
- 资助金额:
$ 1.34万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Cell biological studies on mechanisms of cell-to-cell movement of plant viruses
植物病毒细胞间运动机制的细胞生物学研究
- 批准号:
05660046 - 财政年份:1993
- 资助金额:
$ 1.34万 - 项目类别:
Grant-in-Aid for General Scientific Research (C)
Studies on transcription and replication of viral genomes in infected plant cells by situ hybridization
原位杂交研究受感染植物细胞中病毒基因组的转录和复制
- 批准号:
61560049 - 财政年份:1986
- 资助金额:
$ 1.34万 - 项目类别:
Grant-in-Aid for General Scientific Research (C)
相似海外基金
STRUCTURE AND FUNCTION OF A PEPTIDE OF BROME MOSAIC VIRUS IN MEMBRANE BINDING
雀麦花叶病毒肽的结构和膜结合功能
- 批准号:
7954612 - 财政年份:2009
- 资助金额:
$ 1.34万 - 项目类别:
STRUCTURE AND FUNCTION OF A PEPTIDE OF BROME MOSAIC VIRUS IN MEMBRANE BINDING
雀麦花叶病毒肽的结构和膜结合功能
- 批准号:
7721641 - 财政年份:2008
- 资助金额:
$ 1.34万 - 项目类别:
RNA Recombination at the Subgenomic Promoter in Brome Mosaic Virus: Relationship with Transcription
雀麦花叶病毒亚基因组启动子的 RNA 重组:与转录的关系
- 批准号:
0317039 - 财政年份:2003
- 资助金额:
$ 1.34万 - 项目类别:
Continuing Grant
Genetic Recombination in Brome Mosaic Virus: The Role of Intergenic Region in Junction Site Selection
雀麦花叶病毒的基因重组:基因间区域在连接位点选择中的作用
- 批准号:
9983033 - 财政年份:2000
- 资助金额:
$ 1.34万 - 项目类别:
Continuing Grant
CRYO HRSEM: BROME MOSAIC VIRUS DERIVED VIRUS LIKE PARTICLES FORMED IN YEAST
CRYO HRSEM:雀麦花叶病毒衍生的病毒样颗粒在酵母中形成
- 批准号:
6248522 - 财政年份:1997
- 资助金额:
$ 1.34万 - 项目类别:
Molecular Analysis of the Brome Mosaic Virus RNA-dependent RNA Polymerase
雀麦花叶病毒 RNA 依赖性 RNA 聚合酶的分子分析
- 批准号:
9507344 - 财政年份:1996
- 资助金额:
$ 1.34万 - 项目类别:
Continuing Grant
Mechanism of RNA-RNA Recombination in Brome Mosaic Virus: The Role of Viral Replicase
雀麦花叶病毒 RNA-RNA 重组机制:病毒复制酶的作用
- 批准号:
9305389 - 财政年份:1993
- 资助金额:
$ 1.34万 - 项目类别:
Continuing Grant
Analysis on mechanisms of brome mosaic virus RNA replication
雀麦花叶病毒RNA复制机制分析
- 批准号:
04404009 - 财政年份:1992
- 资助金额:
$ 1.34万 - 项目类别:
Grant-in-Aid for General Scientific Research (A)