STRUCTURE AND FUNCTION OF A PEPTIDE OF BROME MOSAIC VIRUS IN MEMBRANE BINDING

雀麦花叶病毒肽的结构和膜结合功能

• 批准号: 7954612
• 负责人: PAUL G. AHLQUIST
• 金额: $ 0.16万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-03-01 至 2010-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7954612
• 关键词: Binding; Brome mosaic virus; Complex; Computer Retrieval of Information on Scientific Projects Database; Endoplasmic Reticulum; Funding; Grant; Institution; Membrane; Membrane Proteins; Peptides; Peripheral; Polymerase; RNA replication; Recruitment Activity; Research; Research Personnel; Resources; Role; Source; Structure; United States National Institutes of Health; Viral Proteins; peptide A; three dimensional structure; viral RNA

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Brome Mosaic Virus (BMV) protein 1a is a multifunctional RNA replication factor with key roles in assembly and function of viral RNA replication complexes. 1a localizes to perinuclear endoplasmic reticulum (ER) membranes as a peripheral membrane protein, induces ER membrane invaginations in which RNA replication occurs, and recruits BMV 2a polymerase and RNA replication templates to form RNA replication complexes. We identified a peptide in 1a that is critically involved in 1a's functional association with ER membranes. Mutational studies showed that specific residues of the peptide were important for 1a association with membranes. Accordingly, to build on the above results, we will use NMR to determine the three dimensional structure of this peptide and the mechanism of its binding to membranes.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 Brome Mosaic病毒（BMV）蛋白1a是一种多功能的RNA复制因子，在病毒RNA复制复合物的组装和功能中具有关键作用。 1A定位于核周内质网（ER）膜作为周围膜蛋白，引起ER膜起伏，其中发生了RNA复制，并募集BMV 2A聚合酶和RNA复制模板以形成RNA复制复制复合物。我们确定了1A中的肽，该肽与1A的功能相关性与ER膜的功能关联。突变研究表明，肽的特定残基对于1A与膜的关联很重要。因此，为了建立上述结果，我们将使用NMR来确定该肽的三维结构及其与膜结合的机理。