Chemical Studies on Mycoalexin Produced by Fungi

真菌产生的霉菌抗毒素的化学研究

• 批准号: 01560147
• 负责人: UENO Tamio
• 金额: $ 1.34万
• 依托单位: Kyoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1989
• 资助国家: 日本
• 起止时间: 1989 至 1990
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-01560147/
• 关键词: Mycoalexin; Rust Fungus; Anthracnose Fungus; Spore Germination; self-Inhibitor; Antibiotic; Phytotoxin; Aspergillus niger; Bipolaris bicolor; cochlioquiinone; monocerin; Alternaria属; Colletotrichum属; 胞子自己発芽抑制物質; ストレス化合物

This project is originated from our basic chemical studies on fungal spore germination. Spores of a few fungi have been demonstrated to contain endogenous self-inhibitors, some of which were characterized in this project. Other important results were obtained by detailed observations on the behaviour of fungi which were cultivated for a long period under stressed conditions, such as deficiency of nutrients, higher temperature, addition of heavy metals and contamination of other mycroorganisms. In such conditions, some species of fungi produce genuine metabolites, tentatively termed mycoalexins, which seem to be an essential factor related to control their own life cycles. Summary of the results runs as follows :1. Structure of spore germination self-inhibitors from rust fungi and anthracnose fungi were characterized. The structure-activity relationship of the self-inhibitors were investigated by synthetic work.2. Structure of Mycoalexin from a Strain of Aspergillus Niger was Proposed.3. New type of antibiotic and phytotoxdic metabolites were isolated from two strains of Bipolaris bicolor. Their structures were also characterized.4. A new bioassay method has been developed for the detection of mycoalexin.

本课题来源于我们对真菌孢子萌发的基础化学研究。一些真菌的孢子已被证明含有内源性自我抑制剂，其中一些在本项目中进行了表征。其他重要的结果是通过详细观察真菌的行为，这些真菌在胁迫条件下培养了很长一段时间，例如缺乏营养，温度较高，添加重金属和其他微生物的污染。在这种条件下，一些真菌物种会产生真正的代谢产物，暂时称为mycoalexins，这似乎是与控制其自身生命周期相关的重要因素。运行结果总结如下：1.对锈菌和炭疽菌孢子萌发自抑制剂的结构进行了表征。通过合成工作，研究了自抑制剂的构效关系.推测了一株尼日尔曲霉产Mycoalexin的结构.从两株二色平脐蠕孢中分离得到新型抗生素和植物毒素代谢产物。并对其结构进行了表征.建立了一种新的检测霉毒素的生物测定方法。

项目成果

Tamio Ueno, Tetsu Tsurushima, Hiroshi Fukami, Hiroshi Irie: "Chemistry of Self-inhibitors of Fungal Spore Germination" Studies in Natural Products. vol. 10. (1991)

Tamio Ueno、Tetsu Tsurushima、Hiroshi Fukami、Hiroshi Irie：“真菌孢子萌发自抑制剂的化学”天然产物研究。

Tamio Ueno,Tetsu Tsurushima,Hiroshi Fukami,Hiroshi Fukami: "Chemistry of selfーinhibifors of Fungal Spore germinafion" Studies in Natural Proclucts Chemistry. 10. (1991)

Tamio Ueno、Tetsu Tsurushima、Hiroshi Fukami、Hiroshi Fukami：“真菌孢子萌发的自抑制化学”自然产物化学研究 10。（1991）。

Hisanori Tamaki, H. Kumagai, Y. Shimada, T. Ksshima, H. Obata, C. Kim, T. Ueno, T. Tochikura: "Detoxification Metabolism of o-Dinitro-benzene by Yeast Issatchenkia orientalis." Agric. Biol. Chem.vol. 55. 951-956 (1991)

Hisanori Tamaki、H. Kumagai、Y. Shimada、T. Ksshima、H. Obata、C. Kim、T. Ueno、T. Tochikura：“东方伊萨酵母对邻二硝基苯的解毒代谢。”

Shinji Nagai, Hisashi Miyagawa, Tetsu Tsurushima, Tamio Ueno, Hiroshi Fukami: "Phytotoxic Metabolites from Bipolaris bicolor E1-4" Agric. Biol. Chem.vol. 55. (1991)

Shinji Nagai、Hisashi Miyakawa、Tetsu Tsurushima、Tamio Ueno、Hiroshi Fukami：“来自双色双极菌 E1-4 的植物毒性代谢物”Agric。

Tamio Ueno: "Sewndary metabolites related to bost selectim by Plant pathogenic fungi" Pure&Appl.chem.62. 1347-1352 (1990)

Tamio Ueno：“植物病原真菌与 bost selectim 相关的 Sewndary 代谢物” Pure