Development of plant disease diagnositic techniques based on chemical analysis of plant-microbe interactions

基于植物-微生物相互作用化学分析的植物病害诊断技术的发展

• 批准号: 10460048
• 负责人: UENO Tamio
• 金额: $ 8.51万
• 依托单位: KYOTO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B).
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-10460048/
• 关键词: Plant secondary metabolism; Phytotoxin; Potato; Sweet potato; Morning glory; Alternaria; Bipolaris; 二次代謝; エリシター; p-coumaroyloctopamine; rishitin; ナシ黒斑病; AK-toxin; じゃがいも; サツマイモつる割れ病; tyramine; scopolin; scopletin; 宿主特異的毒素

Various types of compounds, such as phytotoxins, defense substances, stress compounds, and pigments, are produced in the diseased plant tissues. It is likely that the composition of these compounds depends on the pathogen and gives a characteristic profile : This research aims to analyze the profiles of low-molecular components in the plant tissues and to develop new plant disease diagnosis techniques.The specific marker components, which are produced in the plant under various stresses, were searched for, using potato, sweet potato and morning glory (laboratory model plant of sweet potato) as the subject plants. Siveral secondary metabolites were identified as the characteristic components in the wounded and pathogen-infected tissues. Further data collection is required to validate the significance of these metabolites as the stress-specific markers.Authentic samples of some phytotoxins are also prepared synthetically or biologically, in order to establish their micro-analytical methods, which are also helpful to identify the pathogens. These included AK-toxins of Alternaria alternata Japanese pear pathotype, AM-toxins of A.alternata apple pathotype, toxins of Bipolaris leaf blight pathogens, and toxins of Fusarium oxysporum f.sp.batatas.

在病害植物组织中产生各种类型的化合物，如植物毒素、防御物质、胁迫化合物和色素。本研究以马铃薯、甘薯和牵牛花(甘薯的实验室模式植物)为研究对象，寻找植物在不同胁迫条件下产生的特异性标志物成分，旨在分析植物组织中低分子成分的分布规律，开发新的植物病害诊断技术。在创伤和病原体感染的组织中，几种次生代谢产物被鉴定为特征成分。还需要进一步的数据收集来验证这些代谢物作为应激特异性标记物的重要性。一些植物毒素的正品也是人工或生物制备的，以建立其微量分析方法，这也有助于病原体的鉴定。其中包括日本梨赤星病菌的AK毒素、苹果赤星病菌的AM毒素、赤霉病病原菌的毒素和烟草枯萎病菌的毒素。

项目成果

Chi-Hwan Lim, Hisashi Miyagawa, Miki Akamatsu, Yoshiaki Nakagawa and Tamio Ueno: "Structures and Biological Activities of Phytotoxins Produced by the Plant Pathogenic Fungus Bipolaris cynodontis cyn A"J.Pestic.Sci.. Vol 23. 281-288 (1998)

Chi-Hwan Lim、Hisashi Miyakawa、Miki Akamatsu、Yoshiaki Nakakawa 和 Tamio Ueno：“植物病原真菌 Bipolaris cynodontis cyn A 产生的植物毒素的结构和生物活性”J.Pestic.Sci.. Vol 23. 281-288 (1998)

Bun-ichi Shimizu Asuka Fujimori, Hisashi Miyagawa, Tamio Ueno, Ken Watanabe and Kei Ogawa: "Resistance against Fusarium Wilt Induced by Nonpathogenic Fusarium in Ipomea tricolor"J.Pesticide Sci.. Vol.25. 365-372 (2000)

Bun-ichi Shimizu Asuka Fujimori、Hisashi Miyakawa、Tamio Ueno、Ken Watanabe 和 Kei Okawa：“三色蕹菜中非致病性镰刀菌诱导的对镰刀菌枯萎病的抗性”J.Pesticide Sci.. Vol.25。

Masahiro Miyashita, Tomoko Nakamori, Takahiro Murai, Hisashi Miyagawa, Miki Akamatsu and Tamio Ueno: "Facile Syntheses of AM-toxins and Analogs as Cyclic Depsipeptides by the Solid-phase Method"Biosci.Biotechnol.Biochem.. Vol.62. 1799-1801 (1998)

Masahiro Miyashita、Tomoko Nakamori、Takahiro Murai、Hisashi Miyakawa、Miki Akamatsu 和 Tamio Ueno：“通过固相方法轻松合成 AM 毒素和类似物作为环状缩肽”Biosci.Biotechnol.Biochem.. 第 62 卷。

Masakazu Okada, Hisashi Miyagawa and Tamio Ueno: "Synthesis of the Methyl Ester of Tritium-labeled AK-toxin I, a Host-specific Toxin Produced by Alternaria alternata Japanese Pear Pathotype"Biosci.Biotechnol.Biochem.. Vol.63. 1253-1260 (1999)

Masakazu Okada、Hisashi Miyakawa 和 Tamio Ueno：“氚标记的 AK 毒素 I 的甲酯的合成，这是一种由链格孢日本梨病型产生的宿主特异性毒素”Biosci.Biotechnol.Biochem.. Vol.63。

Miyashita,M. 他: "Facile Syntheses of AM-toxins and Analogs as Cyclic Depsipeptides by the Solid-phase Method"Bioscience, Biotechnology, and Biochemistry. 62巻9号. 1799-1801 (1998)

Miyashita，M.等人：“通过固相方法轻松合成AM-毒素和类似物作为环状缩肽”，《生物科学、生物技术和生物化学》，第62卷，第9.1799-1801期(1998)。