Development of plant disease diagnositic techniques based on chemical analysis of plant-microbe interactions

在患病的植物组织中产生各种类型的化合物，如植物毒素、防御物质、胁迫化合物和色素。这些化合物的组成可能取决于病原体，并给出了一个特征轮廓：本研究旨在分析植物组织中的低分子成分的轮廓，并开发新的植物疾病诊断技术。以马铃薯、甘薯和牵牛花（甘薯的实验室模式植物）为研究对象，寻找不同胁迫下植物体内产生的特异性标记成分。几种次生代谢物被鉴定为损伤和病原体感染组织中的特征成分。需要进一步的数据收集来验证这些代谢物作为应激特异性标志物的重要性。一些植物毒素的真实样品也被合成或生物制备，以建立其微量分析方法，这也有助于病原体的鉴定。这些毒素分别为日本梨型赤霉的ak -毒素、苹果型赤霉的am -毒素、双极叶枯病菌的毒素和尖孢镰刀菌的毒素。

Various types of compounds, such as phytotoxins, defense substances, stress compounds, and pigments, are produced in the diseased plant tissues. It is likely that the composition of these compounds depends on the pathogen and gives a characteristic profile : This research aims to analyze the profiles of low-molecular components in the plant tissues and to develop new plant disease diagnosis techniques.The specific marker components, which are produced in the plant under various stresses, were searched for, using potato, sweet potato and morning glory (laboratory model plant of sweet potato) as the subject plants. Siveral secondary metabolites were identified as the characteristic components in the wounded and pathogen-infected tissues. Further data collection is required to validate the significance of these metabolites as the stress-specific markers.Authentic samples of some phytotoxins are also prepared synthetically or biologically, in order to establish their micro-analytical methods, which are also helpful to identify the pathogens. These included AK-toxins of Alternaria alternata Japanese pear pathotype, AM-toxins of A.alternata apple pathotype, toxins of Bipolaris leaf blight pathogens, and toxins of Fusarium oxysporum f.sp.batatas.