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Studied on The Immunopathogenesis of Summer-Type Hypersensitivity Pneumonitis

夏型过敏性肺炎的免疫发病机制研究

基本信息

批准号：
01570434
负责人：
ANDO Masayuki
金额：
$ 1.34万
依托单位：
Kumamoto University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1989
资助国家：
日本
起止时间：
1989 至 1990
项目状态：
已结题

项目摘要

To investigate the immunopathogenesis of Japanese summer-type hypersensitivity pneumonitis (SHP), we evaluated the immunopathogenic properties of Trichosporon cutaneum, a major causative agent of the disease. When the culture filtrate antigen of T. cutaneum was chromatographed on DEAE-cellulose, two peaks of polysaccharide, fraction A and B, were obtained. Fraction B was highly reactive to the specific IgG and IgA antibodies in the serum and bronchiol alveolar fluid samples obtained from sensitized rabbits, where fraction A was mainly reactive to the IgG antibodies. When the rabbits sensitized by the intratracheal injection with the particulate anten of T. cutaneum were challenged intratracheally by these antigenic fractions, fraction B induced granulomatous alveolitis, but fraction A induced alveolitis rather than granuloma. Lymphocytes of the mediastinal lymph node responded prominently to fraction B, but less to fraction A, as assessed by proliferative response. Each of the fraction … More s B obtained from two strains of different serotypes was reactive in enzyme-linked immunosorbent assay to the serum samples from the rabbits sensitized with homologous antigen, but far less to that from the rabbits sensitized with heterologous antigen, that is, the antigenic specificity of fraction B was related to the serotype of T. cutaneum. Fraction by gel filtration on Sepharose CL-4B revealed that the molecular weight of the antigenic components in fraction B was larger than 1,000,000 and that the components consisted mainly of polysaccharide. In immunodiffusion, the high molecular weight fractions of serotypes I and II showed precipitin lines reacting with each of the homologous antisera, but no line with the heterologous antisera. This antigenic activity was sodium-perodate-sensitive and pronase insensitive, suggesting a polysaccharide nature. Inhalation challenge test with the serotype-related polysaccharide antigen resulted in positive in SHP patients, but not in exposed asymptomatic family members. Less

为了探讨日本夏季型超敏性肺炎（SHP）的免疫发病机制，我们评估了该疾病的主要病原体皮三磷酸丝虫病的免疫致病特性。在deae -纤维素层析上对皮霉培养滤液抗原进行层析，得到多糖A、B两个峰。B部分对致敏家兔血清和细支气管肺泡液中特异性IgG和IgA抗体有高度反应，其中A部分主要对IgG抗体有反应。经气管内注射皮绦虫颗粒抗原致敏的家兔经气管内攻击后，B部分诱发肉芽肿性肺泡炎，而A部分诱发肺泡炎而非肉芽肿。纵隔淋巴结淋巴细胞对B部分反应显著，但对A部分反应较弱，以增生性反应评估。在酶联免疫吸附试验中，从两株不同血清型菌株中分离得到的s - B对同种抗原致敏兔的血清样品均有较强的反应性，而对异源抗原致敏兔的血清样品则有较弱的反应性，即B组分的抗原特异性与血清型有关。在Sepharose CL-4B上进行凝胶过滤，发现B部分抗原组分分子量大于1,000,000，主要成分为多糖。在免疫扩散中，血清型ⅰ和血清型ⅱ的高分子量部分与每一种同源抗血清均有沉淀线反应，但与异源抗血清无沉淀线反应。该抗原活性对过酸钠敏感，对pronase不敏感，提示多糖性质。吸入激射试验血清型相关多糖抗原在SHP患者中呈阳性，但在暴露的无症状家庭成员中无阳性。少