Development of serological diagnostic kit for summer-type hypersensitivity pneumonitis.

夏季型过敏性肺炎血清学诊断试剂盒的研制

• 批准号: 07557048
• 负责人: ANDO Masayuki
• 金额: $ 5.18万
• 依托单位: KUMAMOTO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07557048/
• 关键词: Summer-type hypersensitivity pneumonitis; Trichosporon; Polysaccharide antigen; Monoclonal antibody; ELISA method; Serological diagnosis; 単クローン抗体

Summer-type hypersensitivity pneumonitis (S-HP) caused by Trichosporon is the most prevalent type of hypersensitivity pneumonitis in Japan, and recently it is also discovered in Korea. In order to facilitate and simplify the diagnosis of S-HP,we attempt to establish sandwich-ELISA system which enables evaluation of specific antibody activity in serum. We obtained the following results.1. Purification of monoclonal antibody (Mab) specific for causative antigenisity was done from ascites of mice, and this Mab can work well as capture antibody.2. Purification of Trichosporon species specific antigen by monoclonal antibody coupled affinity column was done. By using this affinity column, we can obtain an enough amount of antigen to make sandwich-ELISA system.3. We compared with sandwich-ELISA system and ELISA system in which Trichosporon species specific antigen, glucuronoxylomannan, was chemically fixed to ELISA plate. After measured samples, the former system yielded higher sensitivity and time saving.

由毛孢子虫引起的夏季变态反应性肺炎(S-HP)是日本最流行的变态反应性肺炎类型，最近在韩国也发现了它。为了方便和简化S幽门螺杆菌的诊断，我们尝试建立了检测血清中特异性抗体活性的夹心ELISA法。我们得到了以下结果：1.目的：1.从小鼠腹水中分离纯化抗原性特异的单抗，该单抗可以很好地捕获抗体。用单抗偶联亲和层析柱纯化毛孢子菌种特异性抗原。利用该亲和层析柱可以获得足够的抗原量，以制备夹心ELISA系统。比较了夹心法和将毛孢子菌种特异性抗原葡醛酸木甘露聚糖化学固定于酶标板上的酶标法。经样品测试，原系统具有较高的灵敏度和较短的时间。

项目成果

Kazuko Yoshida, et al: "Hypersensitivity pneumonitis induced by a smut fungus-Ustilago esculenta" Thorax. 51. 650-651 (1996)

Kazuko Yoshida 等人：“由黑粉病真菌 - 黑粉菌 (Ustilago esculenta) 诱发的过敏性肺炎”Thorax。

Masayuki Ando et al.: "Summer-type hypersensitivity pneumonitis." Internal Medicine. 34. 707-712 (1995)

Masayuki Ando 等人：“夏季型过敏性肺炎。”

Takanori Mizobe et al: "Purification and characterization of serotype-specific polysaccharide antigen of Trichosporon cutaneum serotype II : A causative antigenic substance of Japanese summer-typehypersensitivity pneumonitis." Clin.Exp.Allergy. 25. 265-27

Takanori Mizobe 等人：“皮肤毛孢子菌血清型 II 血清型特异性多糖抗原的纯化和表征：日本夏季型过敏性肺炎的致病抗原物质。”