Molecular biological study of pathogenesis of the Summer-Type Hypersensitivity Pneumonitis

以前，我们报道过毛孢虫属是夏季型超敏性肺炎（SHP）的主要病原体，SHP是日本最常见的超敏性肺炎（HP）。在这项研究中，我们重新评估了三种类型的抗原在Trichosporon属中作为SHP的病原体。根据与每种培养物的DNA/DNA亲缘关系及其他遗传、生理和形态特征，将相应的标准菌株TIMM 1573（血清I型）、TIMM 1318（血清II型）和M9456（血清III型）分别归属于mucoides、asahii和motevideense三种t .。为了证实这些血清型的意义，从患者环境中分离的98株毛孢菌和24株CBS菌株（包括属内描述的种的型培养物）进行了血清型分型。我们还对220例SHP患者进行了针对Trichosporon spp的血清抗体分析，以估计SHP的抗原谱。更多的结果表明，最常见的三磷病病原菌朝日弓形虫和粘液样弓形虫是SHP的主要病原体。我们还应用聚合酶链反应（PCR）和Southern杂交技术检测小鼠模型或SHP患者支气管肺泡灌洗液（BALF）和患者家中空气中的抗原特异性dna。吸入激射后6 h和96 h，模型BALF阳性率分别为100%和60%。18例患者入院后1周内采集BALF样本，94%为阳性。23名其他疾病患者和7名健康志愿者分别占22%和29%。空气样本由安徒生型小体积空气采样器采集。取样板上的颗粒通过超声波清洗机收集，并通过PCR扩增。2/2的空气样本呈阳性。通过PCR本身或附加的限制性内切酶sau3ai，分别将ashii和mucoides的信号与其他真菌或人细胞区分开来。利用PCR检测宿主和环境中病原体特异性dna，将为微生物暴露引起的职业病发病机制的研究提供一种可扩展的方法。少

Previously, we reported that the genus Trichosporon was a major causative agent of summer-type hypersensitivity pneumonitis(SHP), the most prevalent type of hypersensitivity pneumonitis (HP) in Japan. In this study, we re-valued three antigenic types within the genus Trichosporon as the causative agents of SHP.Corresponding standard strains, TIMM 1573 (serotype I), TIMM 1318 (serotype II), and M9456 (serotype III) were assigned to species T.mucoides, T.asahii and T.motevideense, respectively, based on 95% or more DNA/DNA relatedness with each type of culture and other genetical, physiological and morphological characteristics. To confirm the significance of these serotypes, 98 other strains of Trichosporon isolated from patients' environments and 24 CBS strains, including type cultures of species described within the genus, were serotyped. Serum antibody analysis of 220 SHP patients against Trichosporon spp. were also examined to estimate the antigenic profile of SHP.The present result … More s indicate that T.asahii and T.mucoides, which are the most common causes of trichosporonosis, appear to be the major causative agents of SHP.We also applied polymerase chain reaction (PCR) and Southern hybridization to detect the antigen specific DNAs in the bronchoalveolar lavage fluid (BALF) of a murine model or patients with SHP, and in the air of patients' homes. BALF samples of the model were positive in 100 and 60% at 6 and 96 h after the inhalation challenge, respectively. The BALF samples of 18 patients obtained within 1 week after admission were positive in 94%. Those of 23 patients with other diseases and seven Healthy, volunteers were 22 and 29%, respectively. Air samples were collected by an Andersen type low volume air sampler. Particles on the sampling plates were harvested by a ultrasonic cleaner and amplified by PCR.The 2/2 air samples were positive. The signal of each of T.asahii and T.mucoides were discriminated from the other fungi or human cells by PCR itself or the additional restriction enzyme, Sau3 AI.The detection of pathogen specific DNAs in the host and in the environment by PCR would be an expandable method for the investigation of the pathogenesis of occupational diseases caused by microbial exposures. Less