Development of an ELISA system for diagnosis of summer-type Hypersensitivity pneumonitis and the evaluation of its clinical usefulness

夏季型过敏性肺炎ELISA检测系统的建立及其临床应用评价

• 批准号: 09557055
• 负责人: ANDO Masayuki
• 金额: $ 5.18万
• 依托单位: KUMAMOTO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09557055/
• 关键词: summer-type hypersensitivity pneumonitis; serological diagnosis; ELISA method; Trichosporon asahii; polysaccharide antigen; monoclonal antibody

Summer-type hypersensitivity pneumonitis (SHP) is the most prevalent type of hypersensitivity pneumonitis in Japan. Its main causative antigenic organisms are Trichosporon species (T. asahii and T. mucoides). It is important in diagnosing SHP to prove specific antibody against causative antigen in patient's serum, for the existence of specific antibody indicates the patient had been exposed to, and sensitized to the antigen. Therefore, we attempted to establish a sandwich enzyme-linked immunosorbent assay (ELISA) system which enable evaluation of specific antibody activity in patients' serum samples.In the present study, we devised an ELISA system using monoclonal antibody specific to T. asahii, to measure antibodies against T. asahii. We evaluated the usefulness of the system by using sera from patients with SHP, patients with other pulmonary diseases, or healthy volunteers. We found specificity and sensitivity of the system were 92.8% and 92.3%, respectively. This system was proved to be useful in diagnosing SHP.

夏季型过敏性肺炎（SHP）是日本最常见的过敏性肺炎类型。其主要致病抗原微生物是丝孢酵母属（Trichosporon species，T. asahii和T.粘液样）。SHP患者血清中是否存在特异性抗体是诊断SHP的重要依据，因为特异性抗体的存在表明患者曾接触过该抗原，并对该抗原致敏。因此，我们试图建立一种双抗体夹心酶联免疫吸附试验（ELISA），以评价患者血清中特异性抗体的活性。asahii，检测T.朝平。我们通过使用SHP患者、其他肺部疾病患者或健康志愿者的血清来评估该系统的有用性。本系统的特异性和敏感性分别为92.8%和92.3%。该系统对小水电的诊断有一定的实用价值。

项目成果

溝部孝則、安藤正幸、他: "夏型過敏性肺炎のEnzyme-linked Immunosorbent Assayによる血清診断の有効性の検討"アレルギー. (投稿中).

Takanori Mizobe、Masayuki Ando 等人：“使用酶联免疫吸附测定对夏季型过敏性肺炎进行血清诊断的有效性检查”过敏（进行中）。

T.Mizobe, S.Adachi, A.Hamaoka, M.Ando: "Usefulness of a sandwich ELISA system for the serodiagnosis of summer-type hypersensitivity pneumonitis"Submitted to Allergy.

T.Mizobe、S.Adachi、A.Hamaoka、M.Ando：“夹心 ELISA 系统在夏季型过敏性肺炎血清诊断中的用途”提交给 Allergy。

M.Ando: "Report of the standardization Committee of the Japanese Society of Medical Mycology 1995-1997. Serodiagnostic method of summer-type hypersensitivity pneumonitis"Japanese Journal of Medicine. 40. 239-257 (1999)

M.Ando：“日本医学真菌学会标准化委员会报告1995-1997。夏季型过敏性肺炎的血清诊断方法”日本医学杂志。