A Study of the effect of the reaction between endotoxin and Kupffer cells on the bile congestion.

A.体外实验研究。大鼠肝实质细胞(PC)与非PC细胞(NPC)的相互作用。为了阐明PC在内毒素治疗后早期滞留的机制，本研究采用PC和NPC进行体外实验。结果表明：(1)鼻咽癌与内毒素(ET)或鼻咽癌与内毒素(ET)孵育后上清液共同培养PC时，PC中溶酶体酶或ATPase活性显著升高；(2)经LP 100/24小时内毒素处理的大鼠血清培养PC中上述cmzyme活性也显著升高。(3)在与ET和NPC共同培养过程中产生的PC损伤因子(S)为类蛋白物质(S)，热处理使其作用消失。在这些数据的基础上，提出内毒素血症早期PC损伤因子(S)对PC改变有相当大的影响。II.在C3H/HEN和C3H/HeJ小鼠中PC与NPC的相互作用：(1)在C3H/FEN小鼠和其中一只小鼠的鼻咽癌混合培养中，加入ET可减少PC中~3H-亮氨酸的掺入。(2)PC中~H-亮氨酸掺入也减少，结果与(1)相同。实验研究将内毒素与大鼠鼻咽癌孵育后，经门静脉注入孵育液，造成胆汁淤血和肝功能衰竭模型。因此，建议。胆汁淤血和肝功能衰竭的诱发因素(S)可能是通过ET和鼻咽癌的孵育而释放出来的。

A. Study of experiment in vitroI. Interaction between hepatic parenchymal cell(PC)and non- PC(NPC)in rat.In order to clarify the mechanism of PC impainment in the early stage after endotoxin treatment this study was done under the experiment in vitro by using PC and NPC. The results were as follows ;(1)The lysosomal enzyme or ATPase activities in PC were significantly elevated when PC was cultured with NPC and endotoxin(ET)or with supernatant after the incubation of NPC and ET.(2)Above cmzyme activities in PC were also increased when the culture with serum from rat treated with LP 100 /24hr endotoxin.(3)PC impairment factor(s)produced in the media during the culture with ET and NPC was protein-like substance(s), bcause of the loss of the effects by the heat-treatment. On the basis of these data, it has been suggested that PC impairment factor(s)produced by the incubation with ET and NPC had the considerable effect for PC changes in the early stage of endotoxemia.II. Interaction between PC and NPC in C3H/HeN and C3H/HeJ mouse.(1)The incorporation of 3H-Ieucine in PC was decreased by adding ET in the mixed-culture of NPC of C3H/ffeN mouse and PC of one of those mouse.(2)The incorporation of 3H-leucine in PC was also decreased as the same result in(1), when one a both PC was cultured in the supernatant after the incubation of NPC of C3H/HeN mouse with ET.B. Study of experiment in yivoThe model of bile congestion and hepatic failure was conducted by the infusion of the incubation media after the incubation of ET and rat NPC into portal vein. Therefore, it is suggested. that the inductive factor(s)of bile congestion and/of hepatic failure might have been released by the incubation of ET and NPC.