Clinical and Basic Researches of Hepatic Stem Cells onLiver Regeneration as Defense Mechanism.

[研究1]我们建立了非酒精性脂肪性肝炎(NASH)相关性肝硬变2/3部分肝切除(PH)后急性肝功能衰竭的大鼠模型。在短期和长期的研究中，研究了羟色胺提高模型大鼠存活率的机制。高血压组的总生存期明显长于非高血压组(7天生存率分别为46.7%和7.7%)。术前高压性肝移植可能通过阻止宿主肝细胞加速生长和细胞凋亡而提高NASH相关性肝硬变大鼠的存活率。[研究2]我们开发了软件来计算99mTC-半乳糖体人血清白蛋白(GSA)闪烁显像中肝脏和心脏曲线(ABC)之间的感兴趣像素。计算受试者工作特征曲线下面积(AUC)以评估Child-Pugh B级(ChildB级)、病理性慢性肝炎(CH)和肝硬变(LC)。我们开发了一种新的肝功能指标ABC，用于按顺序计算放射性。

We have achieved the results by the clinical and basic studies.【Study 1】We developed a rat model of acute liver failure after 2/3 partial hepatectomy (PH) for non-alcoholic steatohepatitis (NASH)-related cirrhosis. The mechanism by which HT improved the survival of the model rats was examined in short- and long-term investigations. Overall survival was significantly longer in the HT group than the non-HT group (7-day survival rates: 46.7% and 7.7%, respectively). Preoperative HT might improve the survival of rats with NASH-related cirrhosis after PH by preventing the host hepatocytes from accelerating their growth and falling into apoptosis. 【Study 2】We developed software to calculate the pixels of interest in the area between the hepatic and heart curves (ABC) of 99mTc-galactocyl human serum albumin (GSA) scintigraphy. The area under the receiver operating characteristic curve (AUC) was calculated for evaluation of Child-Pugh score grade B (Child B), pathological chronic hepatitis (CH), and liver cirrhosis (LC). We developed a novel liver functional indicator, the ABC, to count radioactivity in sequence.