Kinetics of Kupffer Cells and Cytokines in rejection of Liver Transplantation.

为了阐明Kupffer细胞和细胞因子在肝移植排斥反应中的动力学，采用体外实验系统或体内模型作为大鼠肝移植实验。研究了脂多糖刺激Kupffer细胞释放细胞因子及肝细胞线粒体atp酶活性的变化。实验动物为Wistar大鼠和LEW大鼠。在体外实验中，分离肝实质细胞（PC）和库普弗细胞（KC），在同一菌株或不同菌株下共培养。在体内实验中。研究KC中Kupffer细胞的分布及IL-1的释放。免疫抑制剂对实验结果的影响如下：(1)不同菌株下PC与KC共培养试验。LPS刺激KC细胞因子释放加速，PC线粒体atp酶活性降低。前一种结果被认为是启动效应。(2)环孢素A和甾体激素（强的松龙和地塞米松）抑制了不同菌株下pc和KC共培养时细胞因子释放的加速条件，表明通过IL-1、TNF和超氧化物。(3)环孢素A和甾体激素在体内的作用与(2)的体外实验结果具有相同的意义。(4)综上所述，不同菌株对大鼠肝移植中KC的作用以及KC细胞因子释放加速条件的抑制作用。

In order to clarify the kinetics of Kupffer cells and cytokines in rejection of liver transplantation, the in vitro experimental system or the in vivo model was utilized as the rat liver transplantation. The release of cytokines from Kupffer cells stimulated by lipopolysaccharide and the activities of hepatocytic mitochondrial ATPase were studied.Animals used in this experiment were Wistar and LEW rats. in the in vitro experiment, hepatic parenchymal cells(PC) and Kupffer cells(KC) were isolated and co-cultured under the same-strains or the different-strains each other. In the in vivo experiment. the distribution of Kupffer cells and the release of IL-1 from KC were studied. Moreover, the effects of the immunosuppressiva on these experimental results were as follows ;(1) In the experiment of the co-culture of PC and KC under the different-strains. the accelerations of cytokines-release from KC stimulated by LPS and the decrease of the activities of PC mitochondrial ATPase were observed. The former results were recongnized as the effects of primings.(2) Cyclosporine A and Steroids (predonisolone and dexamethasone) suppressed the accelerative condition of cytokines-release from KC by the co-culture ofPC and KC under the different-strains, indicating by IL-1, TNF and a superoxide.(3) The effects of cyclosporine A and steroids induced the same significance in vivo as the results in vitro summarized in (2).(4) In summary, the concerning of KC and the suppressive effects of accelerated condition of the cytokines release from KC were suggested in rat liver transplantation under the different-strains.