Basic research objected to establish the highly qualified assessment of hepatic reserve function by utilizing new molecular marker localized in liver

基础研究旨在利用肝脏定位的新分子标记物建立高质量的肝储备功能评估

• 批准号: 19390353
• 负责人: HIRATA Koichi
• 金额: $ 11.98万
• 依托单位: Sapporo Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2007
• 资助国家: 日本
• 起止时间: 2007 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19390353/
• 关键词: 肝予備能評価; 新規分子; 肝不全; 胆汁うっ滞; 接着装置; 肝幹細胞; 細胞移植; プロテアーゼインヒビター; アシアロシンチグラフィー; 肝; 組織幹細胞; 分子マーカー; 肝予備能; トランスポーター; タイト結合蛋白

The researches were composed of three kinds of contents as follows : (1) the studies about low localized molucules utilized for the assessments of hepatic function, (2) the regulation on isolation, identification and proliferation of hepatic stem cells recognized to have key roles and (3) the possibility of gene-transfected hepatocyte or heparic stem cell on the treatment for hepatic dysfunction. Our results showed (1) the logical significance of asialoglycoprotein receptor count method and the distribution of adhesion molecules (especially claudins) on hepatic cell function, (2) successful modification of hepatic stem cells in vitro, and (3) the clinical capability by the cell transplantation utilized gene-transfected hepatocytes or fresh hepatic stem cells.

这些研究主要包括以下三个方面的内容：（1）用于肝功能评价的低定位分子的研究;（2）被认为具有关键作用的肝干细胞的分离、鉴定和增殖的调控;（3）基因转染肝细胞或肝素干细胞治疗肝功能障碍的可能性。结果表明：（1）去唾液酸糖蛋白受体计数方法和粘附分子（尤其是claudins）的分布对肝细胞功能的逻辑意义;（2）肝干细胞的体外修饰成功;（3）利用基因转染的肝细胞或新鲜肝干细胞进行细胞移植的临床能力。

项目成果

Proliferation of Hepatocyte Progenitor Cells Isolated from Adult Human Livers in Serum-Free Medium

• DOI: 10.3727/096368908787236666
• 发表时间: 2008-10
• 期刊: Cell Transplantation
• 影响因子: 3.3
• 作者: K. Sasaki;J. Kon;T. Mizuguchi;Qijie Chen;Hidekazu Ooe;H. Oshima;K. Hirata;T. Mitaka

Transforming growth factor-beta induces epithelial to mesenchymal transition by down-regulation of claudin-l expression and the fence function in adult rat hepat ocytes.

转化生长因子-β 通过下调成年大鼠肝细胞中密蛋白-1 的表达和栅栏功能来诱导上皮细胞向间质细胞的转变。

• 发表时间: 2008
• 期刊: Liver Int 28
• 作者: Kojima T.;et al.
• 通讯作者: et al.

Knockdown of tight junction protein Claudin-2 prevent bile canalicular formation in WIF-B9 cells.

紧密连接蛋白 Claudin-2 的敲低可防止 WIF-B9 细胞中胆管的形成。

• 发表时间: 2008
• 期刊: Histochem Cell Biol 131
• 作者: Son S;et al.
• 通讯作者: et al.

肝硬変合併肝細胞癌に対するTissueLink Dissecting Sealer使用の実際.

TissueLink 解剖密封剂在肝硬化相关肝细胞癌中的实际应用。

• 发表时间: 2007
• 作者: Hokari M;et al.;水口 徹
• 通讯作者: 水口 徹

Hypermethylation-modulated downregulation of claudin-7 expression promotes the progression of colorectal carcinoma

• DOI: 10.1159/000124978
• 发表时间: 2008-01-01
• 期刊: PATHOBIOLOGY
• 影响因子: 5
• 作者: Nakayama, Fumihito;Semba, Shuho;Yokozaki, Hiroshi
• 通讯作者: Yokozaki, Hiroshi