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Studies on New World leishmaniasis and its transmisson

新大陆利什曼病及其传播研究

基本信息

批准号：
02041065
负责人：
HASHIGUCHI Yoshihisa
金额：
$ 17.92万
依托单位：
Kochi Medical School
依托单位国家：
日本
项目类别：
Grant-in-Aid for international Scientific Research
财政年份：
1990
资助国家：
日本
起止时间：
1990 至 1992
项目状态：
已结题

项目摘要

In this fiscal year (1992), the data obtained were analyzed from the view of parasitological, immunological, molecular biological, clinical and pathological points as follows :1. Findings on Andean leishmaniasis and its ecology.(1) Andean leishmaniasis cases were reported from two regions of Ecuador, Huigra (1,300 m a.s.l.) and Alausi (2,300-2,500 m), Department of Chimborazo. In the areas schoolchildren, dogs as reservoir host and vector sandflies were examined.(2) Leishmania parasistes were isolated from Lutomyia ayacuchensis caught in both sites, Alausi and Huigra, and also from two children (1 and 2 year-old females) living in Huigra.2. Molecular biological findiings.(1) Karyotypes of L. mexicana, L. panamensis and L. major-like parasites from Ecuador were analyzed by a pulsed field gel electrophoreses (PFGE). The karyotype variations observd among isolates from distinct regions appear to reflect the species diversity of Leishmania in Ecuador.(2) Polymerase chain reaction (PCR) tec … More hniques have been applied for detection of Leishmania DNA.3. Vector entomological findings.(1) Biting activity and Leishmania infection of sandfly, Lutzomyia spp. collected by four different methods were examined, especially in relation to parous rates.(2) The sandfly fauna of each of nine sites endemic for Leishmania was sampled using a variety of collection methosds.4. Clinico-epidemiological findings on the lowland leishmaniasis(1) Clinical forms of the disease in the Pacific lowland were described in detail.(2) Bacterial and fungal floras in suspected Leishmania ulcers of patients from leishmaniasis endemic areas were studied, in relation to the secondary infection.5. Light and electron microscopical findings.(1) Experimentally infected hamsters with Ecuadorian Leishmania isolates were thoroughly examined from clinico-pathological points of view.(2) Ultrastructural observations on the cutaneous lesions of three patients with leishmaniasis were also examined.6. findings on the treatment of cutaneous leishmaniasis.Leishmanicidal activity of paromomycin, meglumine antimonate and mercury chrome was evaluated in vitro and in vivo for the purpose topical applications to American cutaneous leishmaniasis. Less

从寄生虫学、免疫学、分子生物学、临床和病理学角度对本年度（1992年）的资料进行了分析。安第斯利什曼病及其生态学的发现。(1)厄瓜多尔的两个地区报告了安第斯利什曼病病例，和Alausi（2，300 - 2，500米），钦博拉索省。在该地区的学童，狗作为水库主机和媒介白蛉进行了检查。(2)从Alausi和Huigra两个地点捕获的Lutomyia ayacuchensis中分离出寄生利什曼原虫，还从生活在Huigra的两名儿童（1岁和2岁的女性）中分离出寄生利什曼原虫。分子生物学发现。(1)L.墨西哥湖（L. panamensis和L.厄瓜多尔的主要类寄生虫进行了分析，通过脉冲场凝胶电泳（PFGE）。不同地区分离株的核型变异反映了厄瓜多尔利什曼原虫的物种多样性。(2)聚合酶链反应（PCR）技术 ...更多信息方法已应用于利什曼原虫DNA的检测。媒介昆虫学发现。(1)白蛉的叮咬活动和利什曼原虫感染。对四种不同方法收集的数据进行了检查，特别是与经产率的关系。(2)利什曼原虫特有的9个地点的白蛉区系的采样使用各种收集方法。低地利什曼病的临床流行病学研究（1）详细描述了太平洋低地利什曼病的临床类型。(2)对来自利什曼病流行区的疑似利什曼病溃疡患者的细菌和真菌区系进行了研究，探讨了其与继发感染的关系。光镜和电镜结果。(1)从临床病理学角度对实验感染厄瓜多尔利什曼原虫分离株的仓鼠进行了彻底检查。(2)还对三名利什曼病患者的皮肤病变进行了超微结构观察。6.在体外和体内评价巴龙霉素、葡甲胺锑酸盐和汞Chrome的杀利什曼活性，目的是局部应用于美国皮肤利什曼病。少