Studies on New World leishmaniasis and its transmission

新大陆利什曼病及其传播研究

• 批准号: 05041115
• 负责人: HASHIGUCHI Yoshihisa
• 金额: $ 12.8万
• 依托单位: Kochi Medical School
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for international Scientific Research
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-05041115/
• 关键词: Ecuador; Cutaneous leishmaniasis; Patients; Mephaquin; Plasmotrim; Experimental animals; Oral treatment; Skin biopsy; Antimalarial drugs; 抗マラリア剤; メフロキン; アルテサネート; 電顕病理; アネルギー; エクアドル; サシチョウバエ; 二次感染; Leishmania; 皮膚病変; 分子生物学

In this fiscal year, the following items were mainly carried out : 1) the treatment of cutaneous leishmaniasis patients with antimalarial drugs ; 2) identification of parasites isolated in different endemic areas of Ecuador ; and 3) some laboratory experiments using materials collected in the field.1) When 115 isolates from Ecuador were examined by ELISA using 9 monoclonal antibodies, 66 were L.panamensis ; 18, L.mexicana ; 8, L.major-like ; 4, L.braziliensis ; and 2. L.equatorensis. however, 17 isolates were not identified by the present method.2) When Ecuadorian patients with cutaneous leishmaniasis were treated with Mephaquin and Plasmotrim, antimalarial drugs, the majority of them were curative between 2 and 4 weeks after the commencement of the oral treatment showing 100% cure rate. The results obtained in this study are excellent when compared with the parenteral treatment with antimonials. Therefore, the effects of these antimalarial drugs should be tested for other clinical forms of leishmaniasis such as DCL,VL,MCL and PKDL.3) In laboratory studies using the antimalarial drugs mentioned above, no clear-cut curative effect was observed in mice or other experimental animals. Such a discrepancy between animals and humans might be caused by the difference of the action mode of the drug used.4) Electron microscopic examinations of skin biopsies from treated patients with the antimalarial drugs revealed that there was an interesting morphological change in Leishmania organisms and the infected cells, macrophages.5) In order to know the role of P-glycoprotein from Ecuadorian Leishmania amazonensis, the relating gene was isolated and all the base pair arrangement was sequenced. The results obtained suggested that the gene might be a new P-glycoprotein of the genus Leishmania.

本会计年度主要开展以下项目：1)抗疟药物治疗皮肤利什曼病患者；2)厄瓜多尔不同流行区分离的寄生虫鉴定；3)利用野外采集的材料进行室内实验。1)对115株厄瓜多尔分离株进行9种单克隆抗体ELISA检测，其中66株为巴拿马乳杆菌；18、墨西哥；8、major-like；4、巴西木；和2。L.equatorensis。但有17株菌株未被本方法鉴定。2)厄瓜多尔皮肤利什曼病患者口服抗疟药物Mephaquin和Plasmotrim治疗时，大多数患者在口服治疗后2 ~ 4周内治愈，治愈率100%。本研究的结果与抗生素外注射治疗相比是很好的。因此，这些抗疟药物对其他临床形式的利什曼病（如DCL、VL、MCL和pkdl）的疗效应进行试验。3)在使用上述抗疟药物的实验室研究中，未在小鼠或其他实验动物中观察到明确的疗效。动物和人类之间的这种差异可能是由于所使用药物的作用方式不同造成的。4)使用抗疟药物治疗的患者皮肤活检电镜检查显示利什曼原虫生物和感染细胞巨噬细胞有一个有趣的形态学变化。5)为了解厄瓜多尔亚马河利什曼原虫p -糖蛋白的作用，分离了相关基因并对其碱基对排列进行了测序。结果表明，该基因可能是利什曼原虫属的一种新的p糖蛋白。

项目成果

Hashiguchi,Y.(ed.): "Estudios sobre la Leishmaniasis en al Nuevo Mundo y su Transmision con Especial Referencia al Ecuador.No.1" Kyowa Printing Co.Ltd.Kochi,Japan, 93 (1995)

Hashiguchi,Y.（编辑）：“Estudios sobre la Leishmaniasis en al Nuevo Mundo y su Transmission con Especial Referencia al Ecuador.No.1”Kyowa Printing Co. Ltd.Kochi,Japan, 93 (1995)

Hashiguchi,Y.(ed.): "Studies on New World leishmaniasis and its transmission,with particular reference to Ecuador." Kyowa Printing Co.Ltd.Kochi,Japan, 190 (1994)

Hashiguchi, Y.（编辑）：“新大陆利什曼病及其传播的研究，特别是厄瓜多尔。”

橋口義久: "皮膚リーシュマニア症" 皮膚科の臨床. 4. 1-10 (1996)

Yoshihisa Hashiguchi：“皮肤利什曼病”临床皮肤病学。4. 1-10 (1996)

Hashiguchi,Y.(ed.): "Estudios sobre la Leishmaniasis en el Nuevo Mundo y su Transmision con Especial Referencia al Ecuador.No.2" Kyowa Printing Co.Ltd.Kochi,Japan, 148 (1996)

Hashiguchi,Y.（编辑）：“Estudios sobre la Leishmaniasis en el Nuevo Mundo y su Transmission con Especial Referencia al Ecuador.No.2”Kyowa Printing Co. Ltd.Kochi,Japan, 148 (1996)

Hoshokawa A.: "Seroepidemiological surveys for leprosy in Ecuador" Japanese Journal of Tropical Medicine and Hygiene. 22. 179-184 (1994)

Hoshokawa A.：“厄瓜多尔麻风病血清流行病学调查”日本热带医学和卫生杂志。