Studies on New World leishmaniasis and its transmission

新大陆利什曼病及其传播研究

• 批准号: 62043055
• 负责人: HASHIGUCHI Yoshihisa
• 金额: $ 1.22万
• 依托单位: Kochi Medical School
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Overseas Scientific Research
• 财政年份: 1986
• 资助国家: 日本
• 起止时间: 1986 至 1987
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-62043055/
• 关键词: Leishmaniasis; sandflies; reservoirs; Leishmania isolates; Transmission; Epidemiological surveys; Skin test; ELISA

In order to elucidate epidemiological features of leishmaniasis in Centeal and South Americas, field studies on several aspects of the disease were performed mainly in ecuador. The results obtained are summarized as follows:1. According to the articles published in Ecuador to date, there may be three or foue clinical forms of the disease, cuta- neous cases (ca. 93% of total), mucocutaneous (6 or 7%), and visceral and diffuse cutaneous ones. The majority of cases reported was from the Pacific coast, followed by the Amazon. A few cases were also observed in the Andean highland or the mid-Andes.2. We have isolated eight Leishmania stocks, five from humans and three from wild mammals. Identifications based on results of serodeme typing using monoclonal antibodies revealed that three of the five from humans areL. braziliensis panamensis and all three from wild mammals are L. mexicana amazoninsis. Some stocks from humans and mammals require further investigation until theyare fully character … More ized.3. With regard to the vector and reservoir, one species of Lutzomyia, gomezi, and one mammalian species, Tamandua tetradac tyla, were newly implicated, in addition to species recorded already in Ecuador.5. The skin test and ELISA used in this study were highly sensitive and specific for cutaneous and mucocutaneous forms. These diagnostic tools could be very useful in screening of thedisease in epidemiological surveys.6. Andean Leishmaniasis (uta) in Ecuador was first described from the mid-andes (2,300-2,500 m above sea level). The suspected vector is Lutzomyia petuenses. Bacterial flora was isolated from highland and lowland leishmanial ulcers and examined thoroughly, in relation to evolution of leishmanial ulcers.Thus, most of the findings presented here can be considered as preliminary results of the investigation. Based on these basic data obtained, we hope to further elucidate the epidemiological features of leishmaniasis in the new World, with particular reference to Ecuador, in future field research. Less

为了阐明中美洲和南美洲利什曼病的流行病学特征，主要在厄瓜多尔进行了关于该病几个方面的实地研究。主要研究结果如下：1.根据迄今为止在厄瓜多尔发表的文章，可能有三种或四种临床形式的疾病，皮肤病例（约。93%），粘膜皮肤（6或7%），内脏和弥漫性皮肤。报告的大多数病例来自太平洋沿岸，其次是亚马逊。在安第斯高地或安第斯山脉中部也观察到少数病例。我们已经分离出8个利什曼原虫种群，5个来自人类，3个来自野生哺乳动物。基于单克隆抗体血清型分型结果的鉴定显示，来自人类的5个中有3个是L。braziliensis panamensis和野生哺乳动物的3种均为L.墨西哥亚马逊树一些来自人类和哺乳动物的种群需要进一步的调查，直到它们完全特征化。 ...更多信息 ized.3.关于传病媒介和宿主，除了厄瓜多尔已有记录的物种外，还新发现了一种Lutzomyia gomezi和一种哺乳动物物种Tamandua tetratetratetyla。本研究中使用的皮肤试验和ELISA对皮肤和粘膜皮肤形式具有高度敏感性和特异性。这些诊断工具对流行病学调查中疾病的筛查非常有用.厄瓜多尔的安第斯利什曼病（UTA）首先在安第斯山脉中部（海拔2，300 - 2，500米）被描述。疑似病媒是Lutzomyia petuenses。从高地和低地利什曼溃疡中分离出细菌植物群，并进行了彻底的检查，与利什曼溃疡的演变有关，因此，这里提出的大部分结果可以被认为是调查的初步结果。基于这些基本数据，我们希望在今后的实地研究中进一步阐明新大陆特别是厄瓜多尔利什曼病的流行病学特征。少