Studies on New World leishmaniasis and its transmission

新大陆利什曼病及其传播研究

• 批准号: 63041096
• 负责人: HASHIGUCHI Yoshihisa
• 金额: $ 7.04万
• 依托单位: Kochi Medical School
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Overseas Scientific Survey.
• 财政年份: 1988
• 资助国家: 日本
• 起止时间: 1988 至 1989
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-63041096/
• 关键词: Leishmaniasis; Sandflies; Rreservoirs; Epidemiology; Skin test; 皮内反応; ELISA; 抗原分析

In the present study, American cutaneous and mucocutaneous leishmaniasis was examined. The results obtained are summarized as follows.1. Vector and parasite relationships: in eight Departments of Ecuador where juman leishmaniases are endemic, the phleboto mine sand fly was sampled. A total of 40 species was collected, of which at least 11 represented new records for the country. This record increased the number of sand fly species of Ecuador to 56. In the country, three sand fly species of the genus Lutzomyia, trapidoi, hartmanni and gomezi, hitherto, had been recorded as Leishmania-vectors. In the present study, Lu. ayacuchensis from Andean plateau was found to be positive for the parasite. Monthly examination of the natural infection with Leishmania and the biting activity of the sand fly, Lu. ayacuchensis was performed in Andean leishmaniasis-endemic area, Paute. The results revealed that there is a marked monthly variation in both natural infections and biting activity, of the flie … More s in the area suggesting a high transmission intensity during the rainy season.2. Reservoir host research: In distinct human leishmaniasis-endemic areas, 194 wild and domestic animals were examined, by performing liver punctures, of which 14 or 7.2% were positive for protozoans. A strain from Andean domestic dogs was identified as Le pifanoi but the mojority still remained unidentifiable.3. Comparison of lowland and highland leishmaniasis: Cutaneous manifestations of highland leishmaniasis patients are mild compared to those of lowland patients even in the initial phase of the disease.4. Features of causative agent, Leishmania spp.: 18 Leishmania strains from the Pacific coast and Amazonian lowland patients and 11 from Andean highland were isolated. These isolates were precisely characterized employing serodeme, zymodeme and schizodeme analysis. In the Andes area Le. pifanoi was found, while in the Pacific coast Le panamensis and Le. amazonensis were prevalent and Le. braziliensis, in the Amazon. Less

在本研究中，美国皮肤和粘膜利什曼病进行了检查。所得结果总结如下：1。病媒和寄生虫关系：在厄瓜多尔人类利什曼病流行的8个省，对白蛉矿沙蝇进行了取样。共收集到40种，其中至少11种是该国的新纪录。这一记录使厄瓜多尔的沙蝇种类增加到56种。在国内，迄今为止已记录到的利什曼病媒介有三种沙蝇，分别为Lutzomyia属、trapidoi属、hartmanni属和gomzi属。在目前的研究中，陆。来自安第斯高原的ayacuchensis对寄生虫呈阳性反应。自然感染利什曼原虫的月检及鲁氏沙蝇叮咬活动情况。在安第斯利什曼病流行区Paute进行了ayacuchensis。结果表明，该地区蝇类自然感染和叮咬活动均有明显的月变化，雨季传播强度较高。水库宿主研究：在不同的人类利什曼病流行地区，通过肝穿刺检查了194只野生和家养动物，其中14只（7.2%）原生动物呈阳性。来自安第斯家狗的一种菌株被鉴定为Le pifanoi，但大多数仍然无法鉴定。3 .低地利什曼病与高地利什曼病的比较：高原利什曼病患者的皮肤表现即使在疾病的初始阶段也比低地利什曼病患者轻。病原体利什曼原虫特征：从太平洋沿岸和亚马逊低地患者分离出18株利什曼原虫，从安第斯高原分离出11株利什曼原虫。这些分离物采用血清、酶型和分裂体分析进行了精确的鉴定。在安第斯山脉地区。pifanoi被发现，而在太平洋海岸的Le panamensis和Le。亚马逊人普遍存在。巴西人，在亚马逊。少

项目成果

橋口義久: "中南米のリ-シュマニア症、とくにエクアドルを中心に" 熱帯. 22. 68-82 (1989)

Yoshihisa Hashiguchi：“中美洲和南美洲的利什曼病，特别是厄瓜多尔”热带。 22. 68-82 (1989)

三森龍之: "Identification using isoenzyme electrophoresis and monoclonal antibodies,of Leishmania isolated from humans and wild animals of Ecuador." American Journal of Tropical Medicine and Hygiene. 40. 154-158 (1989)

Tatsuyuki Mimori：“使用同工酶电泳和单克隆抗体鉴定从厄瓜多尔人类和野生动物中分离的利什曼原虫。”美国热带医学和卫生杂志 40. 154-158 (1989)。

Takaoka, H.: "Observations on the validity of the ovarian accessory glands of seven Ecuadorian sand fly species (Diptera: Psychodidae) in determining their parity." Japanese Journal of Tropical Medicine and Hygiene. 17. 149-155 (1989)

Takaoka, H.：“观察七种厄瓜多尔白蛉（双翅目：Psychodidae）的卵巢附属腺在确定其胎次方面的有效性。”

橋口義久: "A review of leishmaniasis in Ecuador." Bulletin of Pan American Health Organization. 24. (1990)

Yoshihisa Hashiguchi：“厄瓜多尔利什曼病回顾。”泛美卫生组织公报 24。（1990 年）

Furuya, M.: "Epidemiological survey of leishmaniasis using skin test and ELISA in Ecuador." Japanese Journal of Tropical Medicine and Hygiene. 17. 331-338 (1989)

Furuya, M.：“在厄瓜多尔使用皮试和 ELISA 进行利什曼病流行病学调查。”