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Cooperative Research in the Field of Plant Bioscience Between Japan and Foreign Countries, Especially European Countries and USA

日本与国外特别是欧美国家在植物生物科学领域的合作研究

基本信息

批准号：
02044022
负责人：
HARADA Hiroshi
金额：
$ 1.92万
依托单位：
University of Tsukuba
依托单位国家：
日本
项目类别：
Grant-in-Aid for international Scientific Research
财政年份：
1990
资助国家：
日本
起止时间：
1990 至无数据
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-02044022/
关键词：
Transformation Gene Expression Secondary Metabolism Glycoproteins Higher Plants International Cooperative Research

项目摘要

Cooperative research between Japan and foreign countries, especially U.S.A. and European countries, was carried out in plant bioscience field.1. On transformation : Several transformation methods for higher plants have already been developed and some successful results have been reported in model plants. However, only limited number of reports are available on transformation of agriculturally important plants. Therefore, cooperative research was carried out on the transformation of potato and sweet potato. Our results showed that Ri plasmid was an adequate vector for introducing foreign genes such as kanamycine resistant and GUS genes. Studies on transformation of Streptocarpus, an important material for investigating flower bud differentiation, has been continued.2. On glycoproteins : A carrot secretory glycoprotein GP57 isolated by Tsukuba group was compared with a similar glycoprotein isolated in Wageningen Agricultural University. The expression pattern of the both glycoproteins is comparable, but antiserum for GP57 did not react to the another. The gene expression of GP57 was examined in FMI of Switzerland. The result showed that GP57 gene expression was wound-inducible and thought to have a physiological function in defense mechanism.3. On control mechanism of gene expression : Expression of agropine synthase gene which was identified on T-DNA of Ti plasmid was controlled by the promoter region of the gene and showed a specificity related to cell division. Homology search on nucleotide sequence among various T-DNA genes was carried out in Leiden University and showed no homology among them.4. On secondary metabolites : Production of some secondary metabolites is related to tissue and organ differentiation. Tropane alkaloid production was directly related to root differentiation. Detailed examination on synthetic pathway of secondary metabolites was carried out in cooperation and some new results were obtained.

日本与国外，特别是美国和欧洲国家在植物生物科学领域开展了合作研究。关于转化：已经开发了几种用于高等植物的转化方法，并在模式植物中报道了一些成功的结果。然而，关于农业上重要植物的转化的报道数量有限。为此，开展了马铃薯与甘薯转化的合作研究。结果表明，Ri载体是一种合适的外源基因导入载体，如卡那霉素抗性基因和GUS基因。作为研究花芽分化的重要材料，链果树的转化研究仍在继续。糖蛋白：与瓦赫宁根农业大学分离的类似糖蛋白进行比较。这两种糖蛋白的表达模式是相似的，但GP57的抗血清对另一种糖蛋白没有反应。在瑞士FMI实验室检测了GP57的基因表达。结果表明，GP57基因的表达是创伤诱导的，被认为在防御机制中具有生理功能。基因表达调控机制：在钛载体T-DNA上鉴定到的阿罗品合成酶基因的表达受该基因启动子区域的调控，并表现出与细胞分裂相关的特异性。在莱顿大学对不同T-DNA基因的核苷酸序列进行同源性搜索，发现它们之间没有同源性。次生代谢物：一些次生代谢物的产生与组织和器官的分化有关。托烷生物碱的产生与根的分化有直接关系。合作对次生代谢物的合成途径进行了详细的研究，得到了一些新的结果。