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Japan-France Cooperative Work in Plant Bioscience

日法植物生物科学合作

基本信息

批准号：
03044027
负责人：
HARADA Hiroshi
金额：
$ 7.36万
依托单位：
University of Tsukuba
依托单位国家：
日本
项目类别：
Grant-in-Aid for international Scientific Research
财政年份：
1991
资助国家：
日本
起止时间：
1991 至 1993
项目状态：
已结题

项目摘要

Cooperative research between Japan and France was carried out in plant bioscience field during 3 years.1. On molecular mechanisms of somatic and zygotic embryogenesis : In Japan, embryogenic cell proteins (ECPs), which were found in embryogenic cells but not in non-embryogenic cells, were detected in carrot. cDNAs encoding some ECPs (ECP31, ECP40, and ECP63) were also isolated from carrot. In France, a partial cDNA clone homologous to ECP31 was isolated from a cDNA bank of immature seeds of Arabidopsis thaliana. To clarify physiological functions of ECPs in embryogenesis, exchanges of genes and information have been continued.2. On signal transaction of phytohormones : In Japan, proteins binding to some phytohormones were isolated from hypocotyls of some plants. A Japanese researcher visited a French institute in 1992 and 1993 and cooperative research on isolation of auxin-binding proteins was done. By the work, an auxin-binding protein was partially purified from grape suspension cells and the purification has been continued.3. On regulation mechanisms of plant gene expression : By the cooperative research between Japan and France, promoter regions of some T-DNA genes of Ti plasmids were isolated and ligated with GUS reporter gene. The chimerical genes were delivered into tobacco. In transgenic tobacco containing the GUS reporter gene with a promoter region of tmr gene (Ptmr-GUS), the GUS expression was not influenced by phytohorome treatments. Analyses of the expression of Ptmr-GUS in carrot somatic embryos have been carrying out.4. On workshops between Japan and France : In 1991, a workshop between Japan and France in plant bioscience field had been held in France. Many researchers in both countries were attended in the workshop and discussed on their research and cooperative works. In 1993, similar workshop had been held in Japan.Researchers in both countries reported the results of cooperative works and discussed on the future collaboration.

日法在植物生物科学领域的合作研究历时3年。 1．关于体细胞和合子胚胎发生的分子机制：在日本，在胡萝卜中检测到了胚胎发生细胞蛋白（ECP），这种蛋白存在于胚胎发生细胞中，但在非胚胎发生细胞中没有。编码一些 ECP（ECP31、ECP40 和 ECP63）的 cDNA 也从胡萝卜中分离出来。在法国，从拟南芥未成熟种子的 cDNA 库中分离出与 ECP31 同源的部分 cDNA 克隆。为了阐明ECPs在胚胎发生中的生理功能，继续进行基因和信息的交流。 2．关于植物激素的信号处理：在日本，从一些植物的下胚轴中分离出与某些植物激素结合的蛋白质。日本研究人员于1992年和1993年访问法国一家研究所，并就生长素结合蛋白的分离进行了合作研究。通过该工作，从葡萄悬浮细胞中部分纯化出了生长素结合蛋白，并仍在继续纯化中。 3．关于植物基因表达的调控机制：通过日本和法国的合作研究，分离出Ti质粒的部分T-DNA基因的启动子区域，并与GUS报告基因连接。将嵌合基因传递到烟草中。在含有带有 tmr 基因启动子区域的 GUS 报告基因 (Ptmr-GUS) 的转基因烟草中，GUS 表达不受植物素处理的影响。胡萝卜体细胞胚中Ptmr-GUS的表达分析正在进行中。 4．关于日法研讨会：1991年，在法国举办了日法植物生物科学领域研讨会。两国众多研究人员参加了研讨会，并就他们的研究和合作工作进行了讨论。 1993年，在日本也举办了类似的研讨会，两国研究人员汇报了合作工作的成果，并对今后的合作进行了讨论。