Japan-France Cooperative Work in Plant Bioscience

日本和法国在植物生物科学领域进行了为期3年的合作研究.体细胞胚和合子胚发生的分子机制：在日本，在胡萝卜中检测到胚性细胞蛋白（ECPs），该蛋白在胚性细胞中发现，而在非胚性细胞中未发现。从胡萝卜中还分离出编码一些ECP（ECP 31、ECP 40和ECP 63）的cDNA。在法国，从拟南芥未成熟种子的cDNA库中分离出与ECP 31同源的部分cDNA克隆。为了阐明ECPs在胚胎发生中的生理功能，基因和信息的交换一直在继续。植物激素的信号传递：日本从一些植物的下胚轴中分离出与某些激素结合的蛋白质。1992年和1993年，一名日本研究人员访问了法国的一个研究所，进行了分离生长素结合蛋白的合作研究。通过以上工作，从葡萄悬浮细胞中部分纯化了生长素结合蛋白，并继续纯化.植物基因表达调控机制研究：通过日本和法国的合作研究，分离了Ti质粒的部分T-DNA基因的启动子区，并将其与GUS报告基因连接。嵌合基因被导入烟草。在转基因烟草中，GUS报告基因与tmr基因的启动子区（Ptmr-GUS），GUS的表达不受植物激素组处理。对Ptmr-GUS基因在胡萝卜体细胞胚中的表达进行了分析.日本和法国之间的研讨会：1991年，日本和法国在植物生物科学领域举行了研讨会。两国的许多研究人员参加了研讨会，并讨论了他们的研究和合作工作。1993年在日本举办了类似的研讨会，两国研究人员报告了合作工作的结果，并讨论了今后的合作。

Cooperative research between Japan and France was carried out in plant bioscience field during 3 years.1. On molecular mechanisms of somatic and zygotic embryogenesis : In Japan, embryogenic cell proteins (ECPs), which were found in embryogenic cells but not in non-embryogenic cells, were detected in carrot. cDNAs encoding some ECPs (ECP31, ECP40, and ECP63) were also isolated from carrot. In France, a partial cDNA clone homologous to ECP31 was isolated from a cDNA bank of immature seeds of Arabidopsis thaliana. To clarify physiological functions of ECPs in embryogenesis, exchanges of genes and information have been continued.2. On signal transaction of phytohormones : In Japan, proteins binding to some phytohormones were isolated from hypocotyls of some plants. A Japanese researcher visited a French institute in 1992 and 1993 and cooperative research on isolation of auxin-binding proteins was done. By the work, an auxin-binding protein was partially purified from grape suspension cells and the purification has been continued.3. On regulation mechanisms of plant gene expression : By the cooperative research between Japan and France, promoter regions of some T-DNA genes of Ti plasmids were isolated and ligated with GUS reporter gene. The chimerical genes were delivered into tobacco. In transgenic tobacco containing the GUS reporter gene with a promoter region of tmr gene (Ptmr-GUS), the GUS expression was not influenced by phytohorome treatments. Analyses of the expression of Ptmr-GUS in carrot somatic embryos have been carrying out.4. On workshops between Japan and France : In 1991, a workshop between Japan and France in plant bioscience field had been held in France. Many researchers in both countries were attended in the workshop and discussed on their research and cooperative works. In 1993, similar workshop had been held in Japan.Researchers in both countries reported the results of cooperative works and discussed on the future collaboration.