Comparative studies on the immunogenic proteins of animal herpesviruses

本研究旨在利用分子生物学技术研究几种动物疱疹病毒糖蛋白的免疫原性。用生物学、免疫学和分子生物学方法检测了包括减毒活疫苗F2株在内的9株猫疱疹病毒1型(FHV-1)的异质性。在免疫印迹分析中，有2株病毒结构蛋白缺失，其相对分子质量为36 kDa，其中1株为疫苗F2株。此外，在限制性内切酶分析中，MluI酶的切割图谱显示野生株和疫苗株之间存在基因组异质性。FHV-1 gp143/108和犬疱疹病毒gp145/112不仅对同源病毒产生高中和抗体，而且对异源病毒也产生高中和抗体。伪狂犬病病毒主要糖蛋白gIII基因在大肠杆菌和空泡病毒载体中表达。确定了识别中和表位的6个区域。

This research project was designed to elucidate the immunogenic characteristics of virus glycoproteins among several animal herpesviruses using molecular biological techniques.Main results obtained in 1992 were as follows.1. Heterogeneity of 9 feline herpesvirus type 1(FHV-1) strains including attenuate vaccine F2 strain was examined by biological, immunological, and molecular biological methods. In immunoblot analysis, a virus-structural immunogenic protein with M.W. of 36 kDa was lacking in 2 strains, one of which was the vaccine F2 strains. Furthermore, when restriction endonuclease analysis, the cleavage patterns with the enzyme MluI showed a genomic heterogeneity between wild and vaccine strains.2. FHV-1 gp143/108 and canine herpesvirus (CHV) gp145/112, elicited high neutralizing antibodies not only to the homologous virus but to the heterologous virus as well.3. Major glycoprotein gIII gene of pseudorabies virus was expressed in Escherichia coli and Vaculovirus vector. Six regions recognizing neutralizing epitopes were determined.