Comparative studies on the immunogenic proteins of animal herpesviruses

动物疱疹病毒免疫原性蛋白的比较研究

• 批准号: 02304029
• 负责人: TAKAHASHI Eiji
• 金额: $ 11.14万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Co-operative Research (A)
• 财政年份: 1990
• 资助国家: 日本
• 起止时间: 1990 至 1992
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-02304029/
• 关键词: Herpesvirus; Virus glycoprotein; Expression of gene; Feline herpesvirus type 1; Canine herpes virus; Pseudorabies virus; ウシヘルペスウイルス1型; オ-エスキ-病ウイルス; 単クロ-ン抗体; gB相同遺伝子; 免疫原性; ウマヘルペスウイルス1型; 糖蛋白; 抗原相同性

This research project was designed to elucidate the immunogenic characteristics of virus glycoproteins among several animal herpesviruses using molecular biological techniques.Main results obtained in 1992 were as follows.1. Heterogeneity of 9 feline herpesvirus type 1(FHV-1) strains including attenuate vaccine F2 strain was examined by biological, immunological, and molecular biological methods. In immunoblot analysis, a virus-structural immunogenic protein with M.W. of 36 kDa was lacking in 2 strains, one of which was the vaccine F2 strains. Furthermore, when restriction endonuclease analysis, the cleavage patterns with the enzyme MluI showed a genomic heterogeneity between wild and vaccine strains.2. FHV-1 gp143/108 and canine herpesvirus (CHV) gp145/112, elicited high neutralizing antibodies not only to the homologous virus but to the heterologous virus as well.3. Major glycoprotein gIII gene of pseudorabies virus was expressed in Escherichia coli and Vaculovirus vector. Six regions recognizing neutralizing epitopes were determined.

本研究利用分子生物学技术对几种动物疱疹病毒糖蛋白的免疫原性进行了研究，主要结果如下：1.应用生物学、免疫学和分子生物学方法对猫疱疹病毒1型（FHV-1）弱毒疫苗F2株等9株FHV-1进行了异源性检测。在免疫印迹分析中，M.W. 2株36 kDa的蛋白质缺失，其中1株为疫苗F2株。此外，当限制性内切酶分析时，酶MluI的切割模式显示野生株和疫苗株之间的基因组异质性. FHV-1 gp 143/108和犬疱疹病毒（CHV）gp 145/112均能诱导产生抗同源病毒和抗异源病毒的高水平中和抗体.伪狂犬病病毒主要糖蛋白gIII基因在大肠杆菌和杆状病毒载体中表达。确定了识别中和表位的六个区域。

项目成果

Okazaki,K. et al.: "Hemadsorptive activity of transfected COS-7 cells expressing BHV-1 glycoprotein gIII" Virology. (1993)

冈崎，K.

Kawaguchi, Y. et al.: "Feline herpesvirus type 1 activates transcription directed by the long terminal repeat of feline immunodeficiency Virus gag in Escherichia coli." Virology. 184. 449-454 (1991)

Kawaguchi, Y. 等人：“1 型猫疱疹病毒在大肠杆菌中激活由猫免疫缺陷病毒 gag 的长末端重复序列指导的转录。”

Okazaki,K.et al.: "BHV‐1 adsorption is mediated by the interaction of glycoprotein gIII with hepalin like moiety on the cell surface" Virology. 182. 666-670 (1991)

Okazaki, K. 等人：“BHV-1 吸附是通过糖蛋白 gIII 与细胞表面肝素样部分的相互作用介导的”病毒学 182. 666-670 (1991)。

Xuan,X.et.al.: "Restriction endonuclease analysis of canine herpesviruses isolated in Japan." Jpn.J,Vet.Sci.52. 1181-1188 (1990)

Xu,X.et.al.：“日本分离的犬疱疹病毒的限制性核酸内切酶分析。”

Limcumpao,J.A.et al.: "Intracellular localization and transport of canine herpesvirus glycoproteins" J.Vet.Med.Aci.53. 517-519 (1991)

Limcumpao，J.A. 等人：“犬疱疹病毒糖蛋白的细胞内定位和转运”J.Vet.Med.Aci.53。