Comparative studies on the immunogenic proteins of animal herpesviruses

本研究项目旨在利用分子生物学技术阐明几种动物疱疹病毒的病毒糖蛋白免疫原性特征。1992年取得的主要成果如下：1。采用生物学、免疫学和分子生物学方法对9株猫疱疹病毒1型（FHV-1）毒株（包括减毒疫苗F2毒株）的异质性进行了研究。免疫印迹分析结果显示，2株毒株缺少一种分子量为36 kDa的病毒结构免疫原性蛋白，其中1株为疫苗F2毒株。此外，当进行限制性内切酶分析时，MluI酶的裂解模式显示出野生株和疫苗株之间的基因组异质性。FHV-1 gp143/108和犬疱疹病毒（CHV） gp145/112不仅对同源病毒也对异源病毒产生高中和抗体。假狂犬病毒主要糖蛋白gIII基因在大肠杆菌和空泡病毒载体上表达。确定了6个识别中和表位的区域。

This research project was designed to elucidate the immunogenic characteristics of virus glycoproteins among several animal herpesviruses using molecular biological techniques.Main results obtained in 1992 were as follows.1. Heterogeneity of 9 feline herpesvirus type 1(FHV-1) strains including attenuate vaccine F2 strain was examined by biological, immunological, and molecular biological methods. In immunoblot analysis, a virus-structural immunogenic protein with M.W. of 36 kDa was lacking in 2 strains, one of which was the vaccine F2 strains. Furthermore, when restriction endonuclease analysis, the cleavage patterns with the enzyme MluI showed a genomic heterogeneity between wild and vaccine strains.2. FHV-1 gp143/108 and canine herpesvirus (CHV) gp145/112, elicited high neutralizing antibodies not only to the homologous virus but to the heterologous virus as well.3. Major glycoprotein gIII gene of pseudorabies virus was expressed in Escherichia coli and Vaculovirus vector. Six regions recognizing neutralizing epitopes were determined.