High resolution 3D visualization of oxygen transport in a single cardiomyocyte.

单个心肌细胞中氧运输的高分辨率 3D 可视化。

• 批准号: 09670037
• 负责人: TAKAHASHI Eiji
• 金额: $ 1.98万
• 依托单位: Yamagata University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09670037/
• 关键词: isolated cardiomyocyte; NADH fluorescence; hypoxia; deconvolution; oxidative metabolism; mitochondria; ischemia; image processing; NADH; 分光法; ミオグロビン; 細胞内酸素分圧; 虚血; 心筋

1. As an extension of the high spatial resolution visualization of intracellular oxygen transport in a single cardiomyocyte, intracellular distribution of NADH autofluorescence, an indicator of cellular oxidative metabolism, was quantitated and visualized with a subcellular spatial resolution.2. In the oxygen conditions similar to those observed in vivo, increase in mitochondrial oxygen consumption up to 7 folds resulted in significant augmentation of NADH fluorescence near the core of a cell.3. Changes in NADH fluorescence near the sarcolemma were further resolved with an aid of the deconvolution technique.4. These results indicate that oxidative metabolism of single cardiomyocytes significantly depends on intracellular diffusional oxygen transport.

1. 作为单个心肌细胞内氧运输的高空间分辨率可视化的延伸，细胞氧化代谢指标NADH自身荧光的细胞内分布以亚细胞空间分辨率进行了定量和可视化。在与体内相似的氧条件下，线粒体耗氧量增加高达7倍，导致细胞核心附近NADH荧光显著增强。在反褶积技术的帮助下，进一步解决了肌膜附近NADH荧光的变化。这些结果表明单个心肌细胞的氧化代谢明显依赖于细胞内弥散氧运输。

项目成果

E.Takahashi: "Direct observation of radial intracellular Po_2 gradients in a single cardiomyocyte of the rats." Am.J.Physiol.275. H225-H233 (1998)

E.Takahashi：“直接观察大鼠单个心肌细胞中径向细胞内 Po_2 梯度。”

E.Takahashi: "Oxyger transport to ischemic cardiac myocytes." Adv. Exp. Med. Biol.411. 145-148 (1997)

E.Takahashi：“氧气输送到缺血性心肌细胞。”

高橋英嗣: "続心臓代謝実験法(細胞内局所酸素測定法)" 六法出版社, 573 (1998)

高桥英次：“连续心脏代谢实验方法（细胞内局部氧测量方法）”六穗出版，573（1998）

高橋英嗣: "興和生命科学振興財団研究報告" 単一心筋細胞内酸素分布の高解像度分光計測法(印刷中),

高桥英俊：《兴和生命科学基金会研究报告》单个心肌细胞内氧分布的高分辨率光谱测量（正在印刷中），

Takahashi E: "Measurement of intracellular oxygen in the heart.(in Japanese)" In : Methods in Myocardial Metabolism, Ed by Nagano M and Imai S,Roppo Publishing, Tokyo. 308-314 (1998)

Takahashi E：“心脏细胞内氧的测量。（日语）”，见：心肌代谢方法，长野 M 和今井 S 编，东京六方出版社。