High resolution 3D visualization of oxygen transport in a single cardiomyocyte.

1.作为单个心肌细胞内氧转运的高空间分辨率可视化的延伸，利用亚细胞空间分辨率定量和可视化细胞内NADH自发荧光（细胞氧化代谢的指标）的分布.在类似于体内观察到的氧条件下，线粒体耗氧量增加高达7倍，导致细胞核心附近的NADH荧光显着增强.在去卷积技术的帮助下，进一步分辨了肌膜附近的NADH荧光的变化.这些结果表明，单个心肌细胞的氧化代谢显着依赖于细胞内扩散氧运输。

1. As an extension of the high spatial resolution visualization of intracellular oxygen transport in a single cardiomyocyte, intracellular distribution of NADH autofluorescence, an indicator of cellular oxidative metabolism, was quantitated and visualized with a subcellular spatial resolution.2. In the oxygen conditions similar to those observed in vivo, increase in mitochondrial oxygen consumption up to 7 folds resulted in significant augmentation of NADH fluorescence near the core of a cell.3. Changes in NADH fluorescence near the sarcolemma were further resolved with an aid of the deconvolution technique.4. These results indicate that oxidative metabolism of single cardiomyocytes significantly depends on intracellular diffusional oxygen transport.