High resolution 3D visualization of oxygen transport in a single cardiomyocyte.

1.作为单个心肌细胞内氧转运的高空间分辨率可视化的扩展，用亚细胞空间分辨率定量和可视化了反映细胞氧化代谢的NADH自发荧光在细胞内的分布。在与体内观察到的氧气条件相似的条件下，线粒体耗氧量增加7倍，导致细胞核心附近NADH荧光显著增强。在去卷积技术的帮助下，肌膜附近NADH荧光的变化得到了进一步的解决。这些结果表明，单个心肌细胞的氧化代谢在很大程度上依赖于细胞内的扩散氧运输。

1. As an extension of the high spatial resolution visualization of intracellular oxygen transport in a single cardiomyocyte, intracellular distribution of NADH autofluorescence, an indicator of cellular oxidative metabolism, was quantitated and visualized with a subcellular spatial resolution.2. In the oxygen conditions similar to those observed in vivo, increase in mitochondrial oxygen consumption up to 7 folds resulted in significant augmentation of NADH fluorescence near the core of a cell.3. Changes in NADH fluorescence near the sarcolemma were further resolved with an aid of the deconvolution technique.4. These results indicate that oxidative metabolism of single cardiomyocytes significantly depends on intracellular diffusional oxygen transport.