Studies on the elimination of Campylobacter jejuni from the intestine of chicken.

本项目的目的是通过控制鸡肠道植物群来消灭空肠弯曲菌。试验结果如下：1.将益生菌枯草芽孢杆菌以0.2%或0.5%的比例添加到鸡场普通日粮中，饲喂1周。2.在空肠弯曲菌的实验感染中，接种枯草B.subtilis和B.cereus均不能完全消除空肠弯曲菌; 3.经口接种鸡盲肠内容物，建立了无菌小鼠肠道植物群。鸡肠道优势植物群如双歧杆菌、消化球菌科、拟杆菌科和梭菌属也是无菌小鼠肠道优势植物群。4.鸡植物群小鼠经口接种空肠弯曲菌后，空肠弯曲菌被清除。而用氯仿处理的鸡盲肠内容物、10倍稀释的<-6>盲肠内容物和厌氧接种鸡盲肠内容物的EG琼脂平板上的细菌菌落悬液接种无菌小鼠，空肠弯曲菌均未被消除。5.从鸡菌群小鼠粪便中分离的肠球菌属、肠杆菌科、梭菌属、拟杆菌科和真杆菌属8株菌经口接种无菌小鼠。在这些无菌小鼠中，空肠弯曲菌未被消除。

The purpose of this project is to eliminate C.jejuni by controlling the intestinal flora from the intestine of chicken. Our results were as follows :1.Bacillus subtilis which is one of probiotics was mixed in normal diet at 0.2 or 0.5% for 1 week in a farm of chicken. Consequently, C.jejuni was eliminated from the intestine of chicken.2.In the experimental infection of C.jejuni, inoculation with B.subtilis and B.cereus were unable to eliminate C.jejuni from the intestine of chicken.3.We were able to establish the intestinal flora of chicken in germfree mice by oral inoculation with their cecal contents. The dominant flora in the intestine of chicken, e.g.Bifidobacterium, Peptococaceae, Bacteroidaceae and Clostridium were also dominant flora in the intestine of germfree mice.4.C.jejuni was eliminated after oral inoculation in chicken flora mice. However, in germfree mice inoculated with chloroform-treated chicken cecal contents, 10^<-6> dilution of cecal contents and the suspension of bacterial colonies on EG agar plats inoculated with cecal contents of chicken anaerobically, C.jejuni was not eliminated.5.Eight strains of Enterococcus, Enterobacteriaceae, Clostridium, Bacteroidaceae, and Eubacterium isolated from feces of chicken-flora mice were inoculated orally into germfree mice. In these gnotobiotic mice, C.jejuni was not eliminated.