Supramolecular Formation in Lipids

脂质中的超分子形成

基本信息

批准号：
02402010
负责人：
HATTA Ichiro
金额：
$ 18.3万
依托单位：
School of Engineering, Nagoya University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (A)
财政年份：
1990
资助国家：
日本
起止时间：
1990 至 1993
项目状态：
已结题

项目摘要

This study aims to get evidence for the basic structure of biomembranes, especially to focus upon the supramolecular structure composed of phospholipids. Ripple structure possesses interesting features from mesoscopic structure appearing in phospholipids. In dipalmitoylphosphatidylcholine, it has been found that the secondary ripple structure appears in a metastable state. Furthermore, the condition of the appearance of the metastable state has been clarified. In a phospholipis/cholesterol system, the temperature dependence of the ripple repeat distance has been explained in terms of that for the normal ripple structure. In a similar system, in which a phospholipid molecule has unsaturated hydrocarbon chains, the overall behavior of the phase diagram is almost common among the phospholipids. In a phospholipid/alcohol system, structural study on the interdigitated phase has been performed systematically by changing the length of hydrocarbon chain in a variety of phospholipids and various alcohols. From the electron density profile in the direction normal to the surface of multilamellar, a model of the interdigitated structure has been proposed. In lauroylphosphatidylcholine, a new liquid crystalline phase has been found between ripple and usual liquid crystalline phases : the new liquid crystalline structure has less disordered hydrocarbon chains than in the usual structure. As a model of biomembranes of an extrinsic protein and lipids, a system of poly(L-lysine) and acidic phospholipis has been studied. The main interaction between them has been found to be electrostatic one.

这项研究旨在获得生物膜基本结构的证据，尤其是专注于由磷脂组成的超分子结构。波纹结构具有出现在磷脂中的介观结构的有趣特征。在二硫二酰磷脂酰胆碱中，已经发现次级连锁结构出现在亚稳态。此外，已经澄清了亚稳态的出现状况。在磷脂/胆固醇系统中，对于正常的连锁结构，已经解释了连锁重复距离的温度依赖性。在类似的系统中，其中磷脂分子具有不饱和烃链，相图的总体行为几乎在磷脂中很常见。在磷脂/酒精系统中，通过改变各种磷脂和各种醇的碳氢化合物链的长度来系统地进行互插相的结构研究。从正常的方向到多层面表面的电子密度曲线，已经提出了互插结构的模型。在月球磷脂酰胆碱中，在纹波和通常的液晶阶段之间发现了一种新的液晶相：与通常的结构相比，新的液晶结构的碳氢化合物链含量较小。作为外在蛋白质和脂质的生物膜模型，已经研究了聚（L-赖氨酸）和酸性磷脂的系统。发现它们之间的主要相互作用是静电。