STUDIES ON THE DEVELOPMENT OF HEMORRHAGIC FEVER WITH RENAL SYNDROME VACCINE USING RECOMBINANT BACULOVIRUS EXPRESSED PROTEIN.

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1) All the neutralizing monoclonal antibodies to Hantaan virus envelope proteins examined so far caused antibody-dependent enhancement of infection to macrophage cell lines with subneutralizing amount of the antibodies. Thus, it seemed to be difficult to separate neutralization specific antigens for preparation of vaccine.2) Recombinant Hantaan virus envelope proteins were successfully expressed by the baculovirus expression system to prepare the viral antigens without any biological hazard from the Hantaan virus infection. The antigenic properties of the expressed and authentic viral antigens were indistinguishable from each other in the reaction patternswith a panel of monoclonal antibodies. However, the recombinant envelope protein induced apparently lowerneutralizing antibodies in mice than that induced with authentic viral antigen.3) Under the limitations described above, further effort which utilize adjuvants in the combination with the recombinant antigens will be required to get high neutralizing antibody.4) To examine the possible roles of the antibody-dependent enhancement of infection in the pathogenesis of hantavirus infection, development of animal models that mimics the symptoms of HFRS will be required in the future study.