STUDIES ON THE DEVELOPMENT OF HEMORRHAGIC FEVER WITH RENAL SYNDROME VACCINE USING RECOMBINANT BACULOVIRUS EXPRESSED PROTEIN.

使用重组杆状病毒表达蛋白开发出血热肾综合征疫苗的研究。

• 批准号: 02806056
• 负责人: ARIKAWA Jiro
• 金额: $ 1.22万
• 依托单位: Hokkaido University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1990
• 资助国家: 日本
• 起止时间: 1990 至 1992
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-02806056/
• 关键词: Hemorrhagic fever with renal syndrome; Hantavirus; Baculovirus; Vaccine; Macrophage; Monoclonal antibody; Animal experiment; Virus infection; マクロファ-ジ; 単クロ-ン性抗体

1) All the neutralizing monoclonal antibodies to Hantaan virus envelope proteins examined so far caused antibody-dependent enhancement of infection to macrophage cell lines with subneutralizing amount of the antibodies. Thus, it seemed to be difficult to separate neutralization specific antigens for preparation of vaccine.2) Recombinant Hantaan virus envelope proteins were successfully expressed by the baculovirus expression system to prepare the viral antigens without any biological hazard from the Hantaan virus infection. The antigenic properties of the expressed and authentic viral antigens were indistinguishable from each other in the reaction patternswith a panel of monoclonal antibodies. However, the recombinant envelope protein induced apparently lowerneutralizing antibodies in mice than that induced with authentic viral antigen.3) Under the limitations described above, further effort which utilize adjuvants in the combination with the recombinant antigens will be required to get high neutralizing antibody.4) To examine the possible roles of the antibody-dependent enhancement of infection in the pathogenesis of hantavirus infection, development of animal models that mimics the symptoms of HFRS will be required in the future study.

1）迄今已检查的所有中和对汉塔病毒包膜蛋白的中和单克隆抗体，导致抗体依赖性增强对巨噬细胞系的感染增强，并具有亚中和抗体的抗体。因此，似乎很难分离中和特异性抗原以制备疫苗。2）重组汉塔病毒包膜蛋白被杆状病毒表达系统成功表达，以制备病毒抗原，而没有汉坦病毒感染的任何生物学危害。在反应图案中，单克隆抗体面板的反应模式下，表达和正宗病毒抗原的抗原特性是无法区分的。然而，重组包膜蛋白在小鼠中显然诱导的抗体显然比用真实病毒抗原引起的抗体低。3）在上述局限性下，进一步的努力将辅助物与重组抗原结合起来，以使抗体的高度中和抗体具有高度的状态。4）汉坦病毒感染，在未来的研究中需要模拟HFR症状的动物模型的发展。

项目成果

MORI,F.: "Pathologi cal studies on central nervous tissues of rats infected with Rattus serotype hantauiws(SR-11strain)" Journal of Velerinary Medicine. B38. 665-672 (1991)

MORI,F.：“感染黄鼠血清型 hantauiws（SR-11 株）的大鼠中枢神经组织的病理学研究”《兽医医学杂志》。

KARIWA,H: "Dovelopment and aeplication of proteinG antibode assay for the detection of antsbody to hantavirus" Journal of Velerinary Medicine. 37. 345-354 (1992)

KARIWA,H：“用于检测蚂蚁体汉坦病毒的 ProteinG 抗体测定的开发和应用”《兽医医学杂志》。

Kariwa, H., Arikawa, J., Takashima, I. and Hashimoto, N.: "Development and application of protein G antibody assay for detection of antibody to hantavirus." Journal of Virological Methods. 37. 345-354 (1992)

Kariwa, H.、Arikawa, J.、Takashima, I. 和 Hashimoto, N.：“用于检测汉坦病毒抗体的 G 蛋白抗体测定的开发和应用。”

KAWAMURA,K: "Susceptiblity of laboratory and wild rodento to Rattus on Apodemus-type hantaviwses." Acta virologica. 35. 54-63 (1991)

KAWAMURA,K：“实验室和野生啮齿类动物对黑线姬鼠型汉塔鼠上的黄鼠的敏感性。”

Yao,J.-S.: "Antibody dependent enhancement of hantavirus infection in macrophage cell lines." Archives of Virology. 122. 107-118 (1991)

Yao，J.-S.：“巨噬细胞系中汉坦病毒感染的抗体依赖性增强。”