Genetical and biochemical characterizations of hemorrhagic fever with renal syndrome virus infection in mice.

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1. SCID mice inoculated i.p. with Hantaan virus and Seoul virus developed systemic infection and died after 32 to 38 days post infection.2. Viral antigen was detected from various organs including kidney. Although no hemorrhagic manifestations was seen in kidney, the SCID mice showed increased level of BUN after infection.3. RT-PCR followed by Nested-PCR method was established for detecting Hantaan virus genome in various organs in infected mice.4. SCID mice (21 days post infection) were passively administered with spleen cells which were prepared from normal BALB/c mice. Seven days after the cell transfer, SCID mice began to produce serum antibody to Hantaan virus. In accordance with the appearance of antibody, BUN levels transiently increased. These results suggest the immune response mediated pathology of Hantavirus infection.These results suggest the possible application of SCID mice as the model for hemorrhagic fever with renal syndrome in human. However, the infected SCID mice did not show any hemorrhagic manifestation nor pathologic changes. Further characterizations with the transfer of immune spleen cells and measurement of cytokines in serum will be required.