Molecular biologic characterization of hatavirus pathogenicity

1. 调查了中国的流行病学状况和疫苗研制现状。交换了中国分离株的核苷酸序列信息和血清诊断方法。汉坦病毒遗传特征的技术资料是从斯洛伐克科学家那里获得的。从美国科学家那里获得了汉坦病毒受体和基因重组病毒表征的技术信息。人工汉坦病毒的构建技术由威斯康星大学兽医学院的科学家获得。关于肾病流行病毒致病性的信息来自赫尔辛基大学的antti Vaheri博士。东南亚流行病学和流行病学状况资料来自泰国科学家。证实GalNac特异性凝集素、DBA和SBA对感染有增强作用。阐明了细胞因子在病毒细胞膜融合过程中的作用。对小鼠的致病性与外周细胞的生长速率有关。这种差异被认为是由包膜蛋白的点突变引起的。克隆了病毒S和病毒M基因组并在哺乳动物细胞中表达。构建了几种不同类型的小基因组，对其转录和翻译作用进行了研究。下一步将需要研究微小基因组的表达与S和M基因组表达的蛋白质之间的关系。

1. Epidemiologic conditions and status of vaccine development in China were investigated. Information regarding to nucleotide sequence of Chinese isolates and diagnostic procedures for serodiagnosis were exchanged.2. Technical information for genetic characterization of hantavirus was obtained from Slovak scientist.3. Technical information for characterization of hantavirus receptor and genetic reassortant virus was obtained from US scientist.4. Techniques for construction of artificial hantavirus was obtained from scientist of Wisconsin University School of Veterinary Medicine.5. Information regarding to Nephropathia epidemica virus pathogenicity was obtained from Dr.Antti Vaheri of Helsinki University.6. Information of epidemiologic and epizootiologic conditions in South East Asia was obtained from scientist in Thai.7. Infection enhancement by the GalNac specific lectin, DBA and SBA was confirmed. Involvement of cellular factor on the virus cell membrane fusion was elucidated.8. Pathogenicity to mice was related to growth rated at peripheral cite. The difference was considered to caused by the point mutation at enveloped protein.9. Viral S and M genome was cloned and expressed in the mammalian cells. Several kinds of mini-genomes were constructed for the examination of role for transcription and translation. For the next step, relationship between the minigenome expression and the proteins expressed by S and M genomes will be required.