Molecular biologic characterization of hatavirus pathogenicity

哈塔病毒致病性的分子生物学特征

• 批准号: 11694228
• 负责人: ARIKAWA Jiro
• 金额: $ 3.97万
• 依托单位: Hokkaido University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B).
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-11694228/
• 关键词: Hemorrhagic fever with renal syndrome; HFRS; hantavirus; zoonosis; virus infection; pathogenicity; genetic reassortant; infection enhancement

1. Epidemiologic conditions and status of vaccine development in China were investigated. Information regarding to nucleotide sequence of Chinese isolates and diagnostic procedures for serodiagnosis were exchanged.2. Technical information for genetic characterization of hantavirus was obtained from Slovak scientist.3. Technical information for characterization of hantavirus receptor and genetic reassortant virus was obtained from US scientist.4. Techniques for construction of artificial hantavirus was obtained from scientist of Wisconsin University School of Veterinary Medicine.5. Information regarding to Nephropathia epidemica virus pathogenicity was obtained from Dr.Antti Vaheri of Helsinki University.6. Information of epidemiologic and epizootiologic conditions in South East Asia was obtained from scientist in Thai.7. Infection enhancement by the GalNac specific lectin, DBA and SBA was confirmed. Involvement of cellular factor on the virus cell membrane fusion was elucidated.8. Pathogenicity to mice was related to growth rated at peripheral cite. The difference was considered to caused by the point mutation at enveloped protein.9. Viral S and M genome was cloned and expressed in the mammalian cells. Several kinds of mini-genomes were constructed for the examination of role for transcription and translation. For the next step, relationship between the minigenome expression and the proteins expressed by S and M genomes will be required.

1.调查了我国禽流感的流行情况和疫苗研制现状。就中国分离株的核苷酸序列和血清学诊断方法进行了交流.汉坦病毒基因特征的技术信息来自斯洛伐克科学家。汉坦病毒受体和遗传抑制病毒的鉴定技术信息来自美国科学家。人工汉坦病毒的构建技术来自威斯康星州大学兽医学院的科学家。从赫尔辛基大学的Antti Vaheri博士处获得了关于肾病病毒致病性的信息。东南亚的流行病学和流行病情况的信息是从泰国的科学家那里获得的。证实了GalNac特异性凝集素、DBA和SBA的感染增强作用。阐明了细胞因子对病毒细胞膜融合的影响.对小鼠的致病性与外周部位的生长有关。这种差异被认为是由包膜蛋白的点突变引起的。病毒S和M基因组被克隆并在哺乳动物细胞中表达。构建了几种微型基因组，用于研究转录和翻译的作用。对于下一步，将需要微型基因组表达与S和M基因组表达的蛋白质之间的关系。

项目成果

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