Molecular biologic characterization of hatavirus pathogenicity

1.对中国地区流行性感冒流行情况和疫苗研制情况进行了调查。交换了中国分离株的核苷酸序列和血清学诊断程序的信息。从斯洛伐克科学家处获得汉坦病毒基因特征的技术信息。汉坦病毒受体和基因重排病毒的鉴定技术信息来源于美国科学家。构建人工汉坦病毒的技术来源于威斯康星大学兽医学院的科学家。有关流行肾病病毒致病性的信息来自赫尔辛基大学的Antti Vaheri博士。东南亚的流行病学和流行病情况的信息是从泰国科学家那里获得的。证实了GalNAc特异性凝集素、DBA和SBA对感染的促进作用。阐明了细胞因子对病毒细胞膜融合的影响。对小鼠的致病性与外周生长有关。这种差异被认为是由被膜蛋白的点突变引起的。克隆了S病毒和M病毒基因组，并在哺乳动物细胞中表达。构建了几种微型基因组，用于研究转录和翻译的作用。下一步，需要了解微型基因组的表达与S和M基因组表达的蛋白质之间的关系。

1. Epidemiologic conditions and status of vaccine development in China were investigated. Information regarding to nucleotide sequence of Chinese isolates and diagnostic procedures for serodiagnosis were exchanged.2. Technical information for genetic characterization of hantavirus was obtained from Slovak scientist.3. Technical information for characterization of hantavirus receptor and genetic reassortant virus was obtained from US scientist.4. Techniques for construction of artificial hantavirus was obtained from scientist of Wisconsin University School of Veterinary Medicine.5. Information regarding to Nephropathia epidemica virus pathogenicity was obtained from Dr.Antti Vaheri of Helsinki University.6. Information of epidemiologic and epizootiologic conditions in South East Asia was obtained from scientist in Thai.7. Infection enhancement by the GalNac specific lectin, DBA and SBA was confirmed. Involvement of cellular factor on the virus cell membrane fusion was elucidated.8. Pathogenicity to mice was related to growth rated at peripheral cite. The difference was considered to caused by the point mutation at enveloped protein.9. Viral S and M genome was cloned and expressed in the mammalian cells. Several kinds of mini-genomes were constructed for the examination of role for transcription and translation. For the next step, relationship between the minigenome expression and the proteins expressed by S and M genomes will be required.