STUDY ON ACTION MECHANISM OF BRASSINOSTEROIDS

油菜素类固醇作用机制的研究

• 批准号: 03305009
• 负责人: YOKOTA Takao
• 金额: $ 10.3万
• 依托单位: TEIKYO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Co-operative Research (A)
• 财政年份: 1991
• 资助国家: 日本
• 起止时间: 1991 至 1993
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-03305009/
• 关键词: BRASSINOSTEROID; BIOSYNTHESIS; GROWTH; MICROTUBLE; BLUE LIGHT; ANTIBRASSINOSTEROID; CONJUGATE; MEMBRANE STABILITY; brassinosteroid; 伸長生長; 植物ホルモン; 耐低温ストレス作用; 木部分化; 摘果効果; アンタゴニスト

1)A biosynthetic pathway of brassinolide (teasterone -> typhasterol -> cstasterone -> brassinolide) is found in both cell cultures and intact plant tissue. 3-Dehydrotesterone was found as an intermediate between teasterone and typhasterol2)In pollen of Lolium perenne, brassinosteroid is distributed in amyloplast as examined by immunocytochemistry.3)A myristate ester of teasterone was identified from lily pollen.4)Biological activity of brassinosteroids in rice leaf was increased by irradiation of blue light which causes unrolling of the rice leaf. However, this is not due to increased level of endogenous brassinosteroids.An antibrassinolide termed KM-01 was isolated from a fungus Drechslera avenae and the chemical structure was determined.Slow-releasing synthetic brassinolides were synthesized and a candidate was seltected for practical agricultural use.7)Conditioning effects of brassinolide on seed germination of Striga and Orobanche were analyzed.8)In contrast to auxin, growth effect of brassinosteroids is related with microtuble behavior and with inner layr of stem tissue.9)Cold tolerance effects of brassinosteroids are based on their stabilizing effects of plant membrane.

1）在细胞培养物和完整植物组织中都发现了油菜素内酯的生物合成途径（茶甾酮 -> 斑黄甾醇 -> 甲睾酮 -> 油菜素内酯）。 3-脱氢睾酮被发现是茶甾酮和伤寒甾醇之间的中间体2)通过免疫细胞化学检测，在黑麦草花粉中，油菜素类固醇分布在造粉体中。3)从百合花粉中鉴定出茶甾酮的肉豆蔻酸酯。4)蓝光照射导致展开，水稻叶片中油菜素类固醇的生物活性增加 的稻叶。然而，这并不是由于内源油菜素类固醇水平增加所致。从真菌Drechslera avenae中分离得到一种抗油菜素内酯KM-01，并确定了其化学结构。合成了缓释合成油菜素内酯，并筛选出一个候选物用于实际农业用途。7)油菜素内酯对独脚金和列当种子萌发的调理作用 8)与生长素不同，油菜素内酯的生长作用与微管行为和茎组织内层有关。9)油菜素内酯的耐冷作用是基于其对植物膜的稳定作用。

项目成果

H.Abe: "Effect of Introducing a lacfone group into typhasterol and teasterone to promote rice eamira inclination" Biosci.Biotech.Biochem.56. 1312-1313 (1992)

H.Abe：“将内酮基团引入到斑甾醇和茶甾酮中以促进水稻 eamira 倾向的效果”Biosci.Biotech.Biochem.56。

H Abe: "Effect of blue light on endogenous brassinosteroid activity and leaf unrolling in rice seedlings" Plant Physiol. (submitted).

H Abe：“蓝光对水稻幼苗内源油菜素类固醇活性和叶片展开的影响”《植物生理学》。

S、Asakawa: "Teasterone 3-myristate:A New type of brassinosteroid deriuatioe in Lilium Longiflorum nuthers" Biosci Biotech Biochem. 58. 219-220 (1994)

S，浅川：“Teasterone 3-肉豆蔻酸酯：百合中的新型油菜素类固醇衍生物”Biosci Biotech Biochem 58. 219-220 (1994)

T.Iwasaki: "Brassinosteroids act as regulators of tracheary element differentiation in isolated Zinnia mesophyll cells" Plant & Cell Physiology. 32. 1007-1014 (1991)

T.Iwasaki：“油菜素类固醇在分离的百日草叶肉细胞中充当气管元件分化的调节剂”植物

Y Takeuchi: "Effects of brassinolide on conditioning and germination of witchweed (Striga asiatica) seeds." Brassinosteroids. Chemistry, Bioactivity & Applications (ed. HG Cutler, T Yokota, G Adam). 298-305 (1991)

Y Takeuchi：“油菜素内酯对巫草（Striga asiatica）种子的调理和发芽的影响。”