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Effect of environmental factors on brassinosteroid biosynyhesis

环境因素对油菜素类固醇生物合成的影响

基本信息

批准号：
13460050
负责人：
YOKOTA Takao
金额：
$ 10.11万
依托单位：
Teikyo University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2001
资助国家：
日本
起止时间：
2001 至 2003
项目状态：
已结题

项目摘要

We investigated the effects of light and temperature on brassinosteroid (BR) biosynthesis as well as fluctuation of BR biosynthesis gene expression during seed growth.1. Effect of light on the biosynthesis of BR in rice,Blue light promoted the synthesis of castasterone and typhasterol in rice, At the same time blue light activated the expression of the OsD WARP gene involved in C6 oxidation responsible for the synthesis of castasterone and typhasterol. Red light and far-red light did not show such a pvomotive activity.2. Effect of temperature on the biosynthesis of BR in rice.The endogenous ERs in rice seedlings grown at 21, 28, 35 and 42℃ were quantified. Compared with 28℃, 35℃ promoted C2α-hydroxylation, while 42℃ suppressed C22-hydroxylation. No such effects were observe in the case of 21℃.3. Fluctuation of pea BR biosynthesis genes during pea seed growth.Pea genes homologous to Arcibidopsis ER biosynthesis genes were cloned and their fluctuation during pea seed growth was examined. We found that the P sDWARF gene is expressed in rapidly growing seeds and is responsible for the synthesis of active ERs such as brassinolide and castasterone. The PsCPD and PsDWF4 genes were found to be responsible for the synthesis of BR intermediates in the early to middle growth stages, In contrast, the level of PsCPD1 transcript was low at the early to middle growth stages but high at the maturing and mature stages, suggesting that 6-deoxocathasteonre accumulated in mature seeds is hydroxylated at C23 by PsCPD1 during early germination and the product is utilized for the synthesis of active BRs, The expression of the PsDET2 gene was found to be constant during seed growth.

研究了油菜籽生长过程中光、温对油菜素内酯（BR）生物合成的影响以及BR生物合成基因表达的变化.光对水稻BR生物合成的影响蓝光促进水稻Castasterone和typhasterol的合成，同时激活了水稻中负责Castasterone和typhasterol合成的C6氧化的OsD WARP基因的表达。红光和远红光则没有这种促进作用.温度对水稻BR生物合成的影响：测定了21、28、35和42℃下水稻幼苗内源ER的含量。与28℃相比，35℃促进C2α-羟基化，而42℃抑制C22-羟基化。在21℃的情况下没有观察到这种影响.豌豆BR生物合成基因在豌豆种子生长过程中的变化本研究克隆了与Arcibidopsis ER生物合成基因同源的豌豆基因，并分析了它们在豌豆种子生长过程中的变化。我们发现，P sDWARF基因在快速生长的种子中表达，并负责合成活性ER，如油菜素和castasterone。PsCPD和PsDWF 4基因在水稻生长的早期和中期负责BR中间产物的合成，而PsCPD 1基因在水稻生长的早期和中期转录水平较低，而在成熟期和成熟期转录水平较高，这表明，6-在成熟种子中积累的deoxocathasteonre在萌发早期被PsCPD 1在C23羟基化，产物用于合成PsDET 2基因的表达在种子生长过程中是恒定的。