Observation of lung injury and repair during growth and development in rats

大鼠生长发育过程中肺损伤及修复的观察

• 批准号: 03670410
• 负责人: KIDA Kozui
• 金额: $ 1.15万
• 依托单位: Tokyo Metropilitan Institute of Gerontology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1991
• 资助国家: 日本
• 起止时间: 1991 至 1992
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-03670410/
• 关键词: Hyperoxia; lung injury; tissue repair; bleomycin injecti-ons; DNA量; 高濃度酵素; DNA含有量; DNA修復,傷害; 肺線維化; DNA polymerse

1. Changes in the DNA content and the activity of DNA polymerase and its alpha and beta forms were studied on the lungs, which were exposured in hyperoxia, of one day prenatal to 42 day postnatal rats. It is concluded that : (1) cellular proliferation in the lung is most active during the first two weeks after birth as supported by increase in both DNA polymerase alpha activity and DNA content, and (2) the capacity for DNA repair, DNA polymerase beta activity throughout the pre and postnatal period remains constant.2. Changes in the DNA synthesis and cellular constituents of mouse lung following repeated bleomycin injections were studied. (1) repeated injections of BLM cause DNA injury in lung cells ; (2) there is a subsequent increase in the DNA repair function as supported by the finding of an increase in DNA polymerase-beta activity ; and (3) these lead further to cell prolifration as supported by the increase in both DNA polymerase-alpha activity and DNA content.

1.本文观察了出生前1天至出生后42天大鼠肺组织DNA含量和DNA聚合酶及其α、β型活性的变化。结论是：（1）肺中的细胞增殖在出生后的前两周最为活跃，这是由DNA聚合酶α活性和DNA含量的增加所支持的，以及（2）DNA修复能力、DNA聚合酶β活性在整个出生前和出生后时期保持恒定。本文研究了博莱霉素重复注射后小鼠肺DNA合成和细胞成分的变化。(1)BLM的重复注射导致肺细胞中的DNA损伤;（2）DNA修复功能随后增加，这得到DNA聚合酶-β活性增加的发现的支持;和（3）这些进一步导致细胞增殖，这得到DNA聚合酶-α活性和DNA含量增加的支持。

项目成果

Katsura H., Taguchi T. and Kida,K: "Alterations in DNA synthesis and cellular constituents in mouse lung following bleomycin injections" Am. J. Respir. Cell Mol. Biol. 6. 190-196 (1992)

Katsura H.、Taguchi T. 和 Kida,K：“注射博莱霉素后小鼠肺部 DNA 合成和细胞成分的变化”Am。

Katsura H,Taguchi T Kida K,: "Alterations in DNA ayutherin and allulon cowtituents in moua lury follouay bleauyain ujectois" An J Resps cell Mol. 6. 190-196 (1992)

Katsura H，Taguchi T Kida K，：“moua lury follouay beauyain ujectois 中 DNA ayutherin 和 allulon cowtituents 的改变”J Resps 细胞 Mol。

Jino S,Kida,K,Taguchi T.: "DNA polymerake β and gaswa in the repor of damajed DNA dway byporoxia lery ujiy in seh" B.B.R.C.

Jino S、Kida、K、Taguchi T.：“DNA 聚合酶 β 和 Gaswa 在 seh 中由 poroxia lery ujiy 损伤的 DNA dway 的报告中”B.B.R.C.

KATSURA H,TAGUCHI T,KIDA K,: "Alferations in DNA syntheis and celbler constituend in mouse lovy follouing bleonyain in jections." Am J Resps Cell Mol. 6. 190-196 (1992)

KATSURA H、TAGUCHI T、KIDA K，：“注射中布洛尼安后小鼠体内 DNA 合成和细胞成分的变化。”

JINNO S,KIDA K,TAGUCHUI T: "DNA polgmerare beta and gamwa in the repair of damafid DNA during bafreoxia buy inguy in red" B.B.R.C.

JINNO S、KIDA K、TAGUCHUI T：“DNA polgmerare beta 和 gamwa 在 bafreoxia 期间修复 damafid DNA 中购买红色的 inguy”B.B.R.C.