A STUDY OF ALTERATIONS OF PLEURAL STRUCTURE BY GROWTH,DEVELOPMENT AND AGING.

生长、发育和衰老引起的胸膜结构改变的研究。

• 批准号: 07670689
• 负责人: KIDA Kozui
• 金额: $ 1.47万
• 依托单位: TOKYO METROPOLITAN INSTITUTION OF GERONTOLOGY.
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07670689/
• 关键词: PLEURA; GROWTH,DEVELOMENT,AND AGING; ELECTRON MICROSCOPIC OBSERVATION; CARAGENNAN PLEURITIS; CYTOKINE.; 加齢変化; 臓側胸膜; マウス; 胸膜中皮細胞

The pleura is not only a mechanical envelope for the lung but also represents a crossroad for the exchange of cells and fluids. We studied ultra-structural alterations during normal aging in the visceral pleura of male mice aged 1 day, 1 wk, 2 wks, 4 wks, 3 mos, 6 mos, 12 mos, 18 mos, 24 mos, and 30 mos, by both scanning and transmission electron microscopy. Surface microvilli (SMV) of mesothelial cells appeared sparse at day 1 (density=D : 18.2/10mum^2), then gradually increased in density and reached a plateau at approximately 18 mos (D : 331.9) when the mesothelial cells appeared bumpy. The length of the SMV changed in parallel with density (day 1=0.23 mm, 1 mos=2.27). After 24 mos, the SMV was partially loose (D=241.3), with an irregular and thin appearance (length : 2.35mum). The morphological properties of pleural connective tissues, including collagen, elastin, and fibroblasts, changed in parallel with the changes in mesothelial cells. Although SMV do not have any known functions in the pleura, they changed with age in a manner that likely corresponded to the changes in pleural connective tissue as well as structural changes in lung parenchyma during the aging process.

胸膜不仅是肺的机械包膜，也是细胞和液体交换的十字路口。我们通过扫描电镜和透射电镜研究了1天、1周、2周、4周、3个月、6个月、12个月、18个月、24个月和30个月雄性小鼠内脏胸膜在正常衰老过程中的超微结构变化。间皮细胞表面微绒毛（SMV）在第1天出现稀疏（密度=D: 18.2/10mum^2），随后密度逐渐增加，在约18 mo时达到平稳（D: 331.9），此时间皮细胞出现凹凸不平。SMV长度随密度平行变化（第1天=0.23 mm, 1 mos=2.27）。24个月后，SMV部分松散（D=241.3），外观不规则且薄（长度：2.35 mm）。胸膜结缔组织的形态学特征，包括胶原蛋白、弹性蛋白和成纤维细胞，与间皮细胞的变化平行变化。虽然SMV在胸膜中没有任何已知的功能，但它们随着年龄的增长而变化，可能与胸膜结缔组织的变化以及在衰老过程中肺实质的结构变化相对应。

项目成果

K.Nomura,K.Kida,et al.: "An ultrastructural study of visceral pleura in mice : special reference to aging changes" Am J Respir Crit Care Med. (in press). (1997)

K.Nomura、K.Kida 等人：“小鼠内脏胸膜的超微结构研究：特别参考衰老变化”Am J Respir Crit Care Med。

野村浩一郎、木田厚瑞: "マウス胸膜の加齢変化の形態学的検討(第1報)" 日本胸部疾患学会誌. 34. 165 (1996)

Koichiro Nomura、Atsuzui Kida：“小鼠胸膜老化变化的形态学研究（首次报告）”日本胸科疾病学会杂志 34. 165（1996）。

伊藤永喜、木田厚瑞ら: "Fischer-344ラットを用いた胸膜炎モデルの検討" 日胸疾会誌. 34. 330-330 (1995)

Eiki Ito、Atsuzui Kida 等：“使用 Fischer-344 大鼠的胸膜炎模型研究”日本胸科学会杂志 34. 330-330 (1995)。

Koichiro Nomura, Kozui Kida., et al.: "An ultrastructural study of visceral pleura in mice : special reference to aging changes." Am J Respir Crit Care Med. (in press).1997

Koichiro Nomura、Kozui Kida. 等人：“小鼠内脏胸膜的超微结构研究：特别参考衰老变化。”

木田厚瑞: "胸腔鏡をめぐる基礎的研究の発展に期待する-Bulla,Blebと肺気腫-" 治療学. 29. 1264-1265 (1995)

Atsuzui Kida：“胸腔镜基础研究发展的期望 - 大疱、疱疹和肺气肿 -” 治疗学 29. 1264-1265 (1995)。