MORPHOLOGICAL CHANGES OF ALVEOLAR CAPILLARY CONFIGURATIONS IN GROWING LUNG.

生长中的肺中肺泡毛细血管结构的形态变化。

• 批准号: 05670543
• 负责人: KIDA Kozui
• 金额: $ 0.45万
• 依托单位: TOKYO METROPOLITAN INSTITUTE OF GERONTOLOGY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05670543/
• 关键词: beta-AMINOPROPIONITRILE; GROWING RAT; ALVEOLAR FORMATION; CAPILLARY CONFIGURATION; EXTRACELLULAR MATRIX; LUNG GROWTH AND DEVELOPMENT; beta-aminopropionctrile; 幼老ラット

beta-AMINOPROPIONITRILE (betaAPN), A LATHYROGEN THAT INHIBITS LYSYL OXIDASE ACTIVITY,WAS REPEATEDLY ADMINISTERED TO MALE RAT PUPS FROM 2 DAYS OF AGE UNTIL SACRIFICE AT FOUR WEEKS OF AGE.BY SCANNING ELECTRON MICROSCOPY,THE LUNGS OF THE TREATED RATS SHOWED A CORROSION CAST OF ALVEOLAR CAPILLARIES THAT APPEARED ABNORMAL IN THICKNESS AND CONFIGURATION ; TRANSMISSION ELECTRON MICROSCOPY SHOWED EXTENSIVE MORPHOLOGICAL CHANGES IN EXTRACELLULAR MATRIC (ECM), INCLUDING COLLAGEN, ELASTIN,GLYCOSAMINOGLYCAN AND INTERSTITIAL CELLS.QUANTITATIVE ANALYSIS REVEALED AN INCREASE IN GLYCOSAMINOGLYCAN OF UP TO 24.4%, A DECREASE IN COLLAGEN (44.5%), AND THE DISAPPEARANCE OF LIPID-LADEN INTERSTITIAL CELLS : HOWEVER,THE CHANGE IN ELASTIN WAS LIMITED TO MORPHOLOGICAL APPEARANCE.FROM THESE OBSERVATIONS,WE CONCLUDE THAT ALTERATIONS IN CAPILLARY CONFIGURATION ARE CONNECTED TO EXTENSIVE CHANGES IN THE ECM,AND THESE CHANGES IN ECM COMPONENTS MIGHT ALSO BE INVOLVED IN THE EMPHYSEMA-LIKE LUNGS INDUCED BY betaAPN.

β -氨基丙腈（β - apn）是一种抑制赖氨酸氧化酶活性的甲状腺原，从2日龄开始反复给予雄性大鼠幼崽直到4周龄时牺牲。扫描电镜观察，大鼠肺泡毛细血管呈腐蚀铸型，厚度和形态异常；透射电镜显示细胞外基质（ecm）发生了广泛的形态学变化，包括胶原蛋白、弹性蛋白、糖胺聚糖和间质细胞。定量分析显示，糖胺聚糖增加高达24.4%，胶原蛋白减少（44.5%），脂质间质细胞消失；然而，弹性蛋白的变化仅限于形态外观。从这些观察中，我们得出结论，毛细血管结构的改变与外膜的广泛改变有关，外膜成分的这些改变也可能与β - apn诱导的肺气肿样肺有关。

项目成果

木田厚端: "肺気腫-序論" The Lung-Perpectives. 1. 1 (1993)

Atsuhata Kida：“肺气肿简介”肺透视。1. 1 (1993)。

緒方 めぐみ、水内 知子、木田 厚瑞、金井 千恵子、野田 幸一: "β-aminopropionitrile.投与ラットにおける肺内微小血管構築の検討" 呼吸. 13. 640 (1994)

Megumi Ogata、Tomoko Mizuuchi、Atsuzui Kida、Chieko Kanai、Koichi Noda：“使用 β-氨基丙腈的大鼠肺内微血管结构的检查”呼吸。 13. 640 (1994)

JINNO S,KIDA K: "EPIDEMIOLOGY OF PULMONARY EMPHYSEMA" THE LUNG-PERSPECTIVES. 1. 33-49 (1993)

JINNO S、KIDA K：“肺气肿的流行病学”肺部视角。

Ogata M,Kida K,Noda K: "A study of lung microvasculer structure in growing rats treated with beta-aminopropionitrile." Proceeding of 4th Annual Lung Development Research Conference. 18 (1993)

Ogata M、Kida K、Noda K：“用 β-氨基丙腈治疗的生长大鼠肺微血管结构的研究。”

木田厚瑞: "肺気腫-序論" The Lung-perspectives-. 1. (1993)

Atsuzui Kida：“肺气肿-简介”肺-观点- 1。（1993）