MORPHOLOGICAL CHANGES OF ALVEOLAR CAPILLARY CONFIGURATIONS IN GROWING LUNG.

从2日龄起反复给雄性大鼠灌胃抑制赖氨酸氧化酶活性的β-氨基丙腈(BetaAPN)，至4周龄处死。扫描电子显微镜显示，处理组大鼠肺泡毛细血管出现腐蚀铸型，厚度和形态异常；透射电子显微镜显示细胞外基质(ECM)有广泛的形态变化，包括胶原、弹性蛋白、糖胺聚糖和间质细胞。定量分析显示糖胺聚糖增加24.4%，胶原减少44.5%，富含脂质的间质细胞消失：然而，弹性蛋白的变化仅限于形态变化。根据这些观察，我们得出结论：毛细血管形态的变化与ECM的广泛变化有关，这些ECM成分的变化也可能与BetaAPN诱导的肺气肿样肺有关。

beta-AMINOPROPIONITRILE (betaAPN), A LATHYROGEN THAT INHIBITS LYSYL OXIDASE ACTIVITY,WAS REPEATEDLY ADMINISTERED TO MALE RAT PUPS FROM 2 DAYS OF AGE UNTIL SACRIFICE AT FOUR WEEKS OF AGE.BY SCANNING ELECTRON MICROSCOPY,THE LUNGS OF THE TREATED RATS SHOWED A CORROSION CAST OF ALVEOLAR CAPILLARIES THAT APPEARED ABNORMAL IN THICKNESS AND CONFIGURATION ; TRANSMISSION ELECTRON MICROSCOPY SHOWED EXTENSIVE MORPHOLOGICAL CHANGES IN EXTRACELLULAR MATRIC (ECM), INCLUDING COLLAGEN, ELASTIN,GLYCOSAMINOGLYCAN AND INTERSTITIAL CELLS.QUANTITATIVE ANALYSIS REVEALED AN INCREASE IN GLYCOSAMINOGLYCAN OF UP TO 24.4%, A DECREASE IN COLLAGEN (44.5%), AND THE DISAPPEARANCE OF LIPID-LADEN INTERSTITIAL CELLS : HOWEVER,THE CHANGE IN ELASTIN WAS LIMITED TO MORPHOLOGICAL APPEARANCE.FROM THESE OBSERVATIONS,WE CONCLUDE THAT ALTERATIONS IN CAPILLARY CONFIGURATION ARE CONNECTED TO EXTENSIVE CHANGES IN THE ECM,AND THESE CHANGES IN ECM COMPONENTS MIGHT ALSO BE INVOLVED IN THE EMPHYSEMA-LIKE LUNGS INDUCED BY betaAPN.