Study of Compensatory Lung Growth Following Pneumonectomy on Rats.

大鼠肺切除术后代偿性肺生长的研究。

• 批准号: 01570437
• 负责人: KIDA Kozui
• 金额: $ 1.34万
• 依托单位: Tokyo Metropolitan Center of Gerontology.
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1989
• 资助国家: 日本
• 起止时间: 1989 至 1990
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-01570437/
• 关键词: Compensatory Lung Growth; Rat; Morphometry; DNA Metabolism; Collagen; Elaston; collagen; elasten; 肺葉切除後代償機転; 形態計測; 生化学的分析

Present study was aimed to elucidate following :1) Both of biochemical and morphological studies of early changes of compensatory lung growth following pneumonectomy on rats.2) Effects of age which may contribute to change of compensatory lung growth.3) Local difference of response in the compensatory lung growth.Two different experiments were performed :Experiment 1 : Fisher-344, male rats of 4 week-old, were divided two groups : Pneumonectomy group (PN group) : left pneumonectomy was performed. Sham operated group (SH group) : left chest was opened similar to PN group, but pneumonectomy was not performed. Morphological study including light morphometry and scanning electron microscopic observation and biochemical studies were performed.Experiment 2 : Sprague-Dawley, male rats of 2 week-old, were divided similar to Experiment 1 and the same morphological and biological studied were performed.In the results of Experiment 1 the wet weight of the contralateral lungs of the pneuminectomiz … More ed rats reached the same level as both lungs of the untreated and sham-operated rats 14 days after operation. DNA polymerase activity, thymidine kinase activity and ^3H-thymidine incoroporation into DNA were measured DNA polymerase activity in the extracts of the contralateral lungs from pneumonectomized rats showed 2 peaks and was elevated on days, 1, 2, and 7. Changes in thymidine kinase activity showed similar changes but was elevate on days 1, 3, and 7. Phosphocellulose column chromatography showed a large peak of DNA polymerase alpha activity. The activity in the lung extracts of pneumonectomized rats was higher than in lung extracts of sham-operated rats on days 3 and 7. A second enzyme peak was due to DNA polymerase beta, and was found in the lungs of both groups. ^3H-thymidine incorporation was increased on day 3 and produced a subsequent increase in DNA content on day 7 in the contralateral lungs of the pneumonectomized rats. DNA synthesis increased in each lobe especially in the cardiac lobe. No changes in the activities of DNA polymerase, thymidine kinase, or ^1H-thymidine incorporation were observed in the untreated control rats.From these observations we conclude that : 1) changes in DNA synthesis and the activities of related enzymes in the contralateral lung occur early in the post-pneumonectomy period, on the first day after the operation, and 2) increased DNA synthesis in all lobes of the remaining lung suggests that humoral factors affect compensatory lung growth but marked increase of ^3H-thymidine incorporation in the cardiac lobe indicates that local mechanical stimukation is as important as humoral factors in the early events.From the observations of pneumonectomy on immature rats (Experiment 2), process of the compensation which included the lung volume, weight and lung morphometry was found similar to the case of adult rats. In addition, it was found that cardiac lobe in the contra-lateral lung showed the most active cellular proliferation compared with those other upper, lower, and middle lobes. Thus, it suggest in the compensatory lung growth, inter-lobar difference may exist. Less

本研究旨在探讨大鼠全肺切除后早期肺代偿性生长变化的生化和形态学研究;年龄对肺代偿性生长变化的影响;肺代偿性生长的局部反应差异。假手术组（SH组）：同PN组一样开胸，但不做全肺切除。实验2：取2周龄雄性SD大鼠，按实验1方法分组，进行形态学和生物学研究。实验1结果：取肺切除大鼠对侧肺湿重为1.25 ± 0.25 μ g/kg，对照组为1.25 ± 0.25 μ g/kg，实验2结果：取肺切除大鼠对侧肺湿重为1.25 ± 0.25 μ g/kg，对照组为1.25 ± 0.25 μ g/kg，实验2结果：取肺切除大鼠对侧肺湿重为1.25 ± 0.25 μ g/kg，对照组为1.25 ± 0.25 μ g/kg，实验3结果：取肺切除大鼠对侧肺湿重为1.25 ± 0.25 μ g/kg，实验4结果：取肺切除大鼠对侧肺湿重为1.25 ± 0.25 μ g/kg，对照组为1.25 ± 0.25 μ g/kg，实验4结果：取肺切除大鼠对侧肺湿重为1.25 ...更多信息 艾德组大鼠术后14天左右肺组织的病理学改变与假手术组和未处理组相同。检测了DNA聚合酶活性、胸苷激酶活性和^3H-胸苷掺入DNA的情况。肺切除大鼠对侧肺提取物中的DNA聚合酶活性显示出两个峰值，并在第1、2和7天升高。胸苷激酶活性的变化显示出类似的变化，但在第1、3和7天升高。磷酸纤维素柱层析显示DNA聚合酶α活性的大峰。在第3天和第7天，肺切除大鼠肺提取物中的活性高于假手术大鼠肺提取物中的活性。第二个酶峰是由于DNA聚合酶β，并发现在两组的肺。在肺切除大鼠的对侧肺中，^3H-胸苷掺入在第3天增加，随后在第7天导致DNA含量增加。各叶DNA合成增加，尤其是心叶。在未处理的对照大鼠中，未观察到DNA聚合酶、胸苷激酶或^1H-胸苷掺入的活性发生变化。1）对侧肺DNA合成和相关酶活性的变化发生在肺切除术后早期，即术后第一天，2）其余肺叶的DNA合成增加表明体液因素影响代偿性肺生长，但^3H-胸苷掺入心叶表明，在早期事件中，局部机械刺激与体液因素同样重要。对未成年大鼠肺切除的观察（实验2），发现代偿过程包括肺体积、重量和肺形态计量学，与成年大鼠的情况相似。另外，对侧肺的心叶细胞增殖最活跃，而上、下、中叶细胞增殖最活跃。提示在代偿性肺生长中可能存在叶间差异。少

项目成果

Mizuuchi T,Mizuuchi A,Taguchi T,Seyama Y,Kida K: "Early changes of postpneumonectany lung growth in twoーweek old rats:morphological and biochenial studies" American Review of Respiratory Disease.

Mizuuchi T、Mizuuchi A、Taguchi T、Seyama Y、Kida K：“两周大大鼠肺炎后肺部生长的早期变化：形态学和生物化学研究”美国呼吸疾病评论。

水内厚,水内知子,木田厚瑞.: "肺葉切除ラット・モデルにおける肺胞再生機序に関する研究 第1報 残存肺の形態学的検討" 日本胸部疾患学会誌. 27. 143 (1989)

Atsushi Mizuuchi、Tomoko Mizuuchi、Atsuzui Kida.：“肺叶切除大鼠模型中肺泡再生机制的研究。首次报告：剩余肺的形态学检查”日本胸科疾病学会杂志 27. 143 (1989)。

水内 知子,木田 厚瑞,田口 隆彦,瀬山 義幸: "幼若ラットにおける肺葉切除後の再生について" 日本胸部疾患学会誌. 29. 11-15 (1991)

Tomoko Mizuuchi、Atsuzui Kida、Takahiko Taguchi、Yoshiyuki Seyama：“幼鼠肺叶切除术后的再生”日本胸科疾病学会杂志 29. 11-15 (1991)。

水内知子,水内厚,田口隆彦,木田厚瑞: "生後2週齢ラットにおけるー側肺切除後の代償性変化の研究 第2報 残存肺の生化学的研究" 日本胸部疾患学会誌. 28. 161 (1990)

Tomoko Mizuuchi、Atsushi Mizuuchi、Takahiko Taguchi、Atsuzui Kida：“2周龄大鼠阴性肺切除术后代偿性变化的研究，第二次报告：残留肺的生化研究”日本胸科疾病学会杂志28。 161 (1990)

水内 厚: "肺葉切除後ラットにみられる残存肺の形態学的変化" 日本老年病学会誌.

Atsushi Mizuuchi：“肺叶切除术后观察到的大鼠残余肺的形态变化”日本老年医学会杂志。