Experimental study of lymph node metastasis on oral cancer

口腔癌淋巴结转移的实验研究

• 批准号: 03670939
• 负责人: SHINGAKI Susumu
• 金额: $ 1.28万
• 依托单位: Niigata University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1991
• 资助国家: 日本
• 起止时间: 1991 至 1992
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-03670939/
• 关键词: Oral cancer; Lymph node metastasis; Hamster; Cell culture; Organ specificity; 扁平土皮癌; 増殖因子; ハムスタ-; 扁平上皮癌; 継代培養; 株化

A cell line with a high metastatic potency to regional lymph nodes was established from a squamous cell carcinoma(O-1N)metastatic to a submandibular lymph node of a hamster. The O-1N was established by serial trarnsplantation of a metastatic tumour to the submandibular lymph node from a chemically induced squamous cell carcinoma of the tongue into the pouch in hamster. Cultured cells which constantly grew in a monolayred sheet have been maintained through 72 passage over 35 months. Inoculation of cells of passage 7 and 18 into the buccal pouch of 10 and five hamsters, respectively, resulted in the production of buccal tumours with lymph node metastases in all animals. The findings were essentially consistent with those exhibited by O-1-N in an in vivo model.To test the effect of organ-tissue-derived factors on the survival and growth of O-1N of lymph node colonization property we used the hamster tissues : lung and lymph nodes. Organ tissue pieces were prepared, and the conditioned media from suspensions cultures of these tissues in low serum concentrations were incubated with O-1N.Replating O-1N cells in medium with low serum content allow only minimal growth. Addition of the organ-conditioned medium to O-1N cells had minimal effects on O-1N cell growth.

从仓鼠颌下腺淋巴结转移的鳞状细胞癌（O-1N）建立了具有高转移性的区域淋巴结细胞系。O-1N是通过将化学诱导的舌鳞状细胞癌转移瘤连续移植到仓鼠的下颌骨淋巴结建立的。在单层膜中不断生长的培养细胞已维持了35个月72代。将7代和18代细胞分别接种到10只和5只仓鼠的颊袋中，所有动物都产生了伴有淋巴结转移的颊肿瘤。这些发现与O-1-N在体内模型中的表现基本一致。为了检测器官组织源性因子对O-1N存活和生长的影响，我们采用仓鼠肺和淋巴结组织进行定殖。制备器官组织块，用低血清浓度的组织悬液培养的条件培养基与O-1N孵育。在低血清含量的培养基中复制O-1N细胞只允许少量生长。在O-1N细胞中添加器官条件培养基对O-1N细胞生长的影响很小。