Experimental study of lymph node metastasis on oral cancer

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A cell line with a high metastatic potency to regional lymph nodes was established from a squamous cell carcinoma(O-1N)metastatic to a submandibular lymph node of a hamster. The O-1N was established by serial trarnsplantation of a metastatic tumour to the submandibular lymph node from a chemically induced squamous cell carcinoma of the tongue into the pouch in hamster. Cultured cells which constantly grew in a monolayred sheet have been maintained through 72 passage over 35 months. Inoculation of cells of passage 7 and 18 into the buccal pouch of 10 and five hamsters, respectively, resulted in the production of buccal tumours with lymph node metastases in all animals. The findings were essentially consistent with those exhibited by O-1-N in an in vivo model.To test the effect of organ-tissue-derived factors on the survival and growth of O-1N of lymph node colonization property we used the hamster tissues : lung and lymph nodes. Organ tissue pieces were prepared, and the conditioned media from suspensions cultures of these tissues in low serum concentrations were incubated with O-1N.Replating O-1N cells in medium with low serum content allow only minimal growth. Addition of the organ-conditioned medium to O-1N cells had minimal effects on O-1N cell growth.