Study of Free Radicals in Tissue and Transdermal Drug Delivery by using in vivo ESR with Spectral-Spatial ESR-CT Imaging

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The several techniques for ESR imaging have been developed. The conventional methods for ESR-CT are based on the assumption that only one spectralcomponetn is existing in all space observed. No image can be constructed if more than two signal componets are coexisting in different part. In order to accomplish the image construction from plural signal components, we tried to connect spectral-spatial imaging technique with CT imaging.1. We report the ESR-CT imaging by the phantom involving two radical species giving different signals. Spectral-spatial images obtained on four derections in X-Z plane. The spatial information was separated from the spectral-spatial images by each radical component. Two separation methods were compared. In one method, separation could not be complete. The border was broadened in other method. Two sets of CT images of two signal components were reconstructured. CT images have good agreement with arrangement of phantom.2. The phantom involving liposomes which encapsulated nitroxide (150 mM) and free nitroxide (3.3mM) was made. We obtained the ESR-CT imaging by L-band ESR.CT images have good agreement with the distribution of loposomes and free nitroxide.These results demonstrate the possibility of ESR-CT for the sample including plural signal components.