Roles of in vivo REDOX systems by using in vivo ESR and transgenic mice

使用体内 ESR 和转基因小鼠体内氧化还原系统的作用

• 批准号: 07457530
• 负责人: UTSUMI Hideo
• 金额: $ 4.86万
• 依托单位: KYUSHU UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07457530/
• 关键词: Transgenic animals; Electron spin resonance; antioxidative enzymes; Superoxide dismutase; 遺伝子組換え動物; フリーラジカル; 電子スピン共鳴法; 抗酸化剤; 生体レドックス; 活性酸素; ESR; スーパーオキシド; 遺伝子組換え

Reactive oxygen species and free radicals have important roles in physiological phenomena and diseases. There are discrepancy in the roles of antioxidative enzymes or antioxidants between in vitro and in vivo experiments. It is, therefore, important to investigate in vivo free radical reactions, to clarify roles of in vivo redox enzymes.In this study, role of Cu/Zn SOD was investigated under several kinds of oxidative stress using electron spin resonance (ESR) technique and Cu/Zn-SOD transgenic mice. In vivo ESR technique is the unique detection method to measure in vivo free radical reactions non-invasively.In physiological condition, it was indicated using newly synthesized redox probe that redox balances in SOD-Transgenics was changed. Overexpression of Cu/Zn-SOD on free radical reactions caused by 2,4,6-trichlorophenol (TCP) was also investigated using Cu/Zn-SOD transgenic mice and in vivo ESR spectroscopy. TCP enhanced free radical reactions in upper abdomen and increased serum GOT in a dose dependent manner. These enhancements were suppressed both by antioxidant and by overexpression of Cu/Zn-SOD,suggesting the relation of free radicals with liver injury caused by TCP and the suppression effect of endogenous Cu/Zn-SOD.Results of this study indicates that combination of transgenic animals and in vivo ESR technique will be useful to clarify the role of redox enzymes in diseases.

活性氧和自由基在生理现象和疾病中具有重要作用。抗氧化酶或抗氧化剂在体内和体外实验中的作用存在差异。本研究以Cu/Zn-SOD转基因小鼠为研究对象，采用电子自旋共振（ESR）技术，研究了Cu/Zn-SOD在几种氧化应激下的作用。体内ESR技术是一种独特的无创检测体内自由基反应的方法，在生理条件下，利用新合成的氧化还原探针表明SOD-转基因生物体内的氧化还原平衡发生了改变。利用转Cu/Zn-SOD基因小鼠和体内ESR波谱研究了Cu/Zn-SOD过表达对2，4，6-三氯苯酚（TCP）引起的自由基反应的影响。TCP可剂量依赖性地增强上腹部的自由基反应和升高血清GOT。抗氧化剂和过量表达Cu/Zn-SOD均能抑制这些增强作用，提示自由基与TCP所致肝损伤的关系以及内源性Cu/Zn-SOD的抑制作用。本研究结果表明，转基因动物和体内ESR技术的结合将有助于阐明氧化还原酶在疾病中的作用。

项目成果

H.Utsumi et al.: "n Vivo ESR Measurement of Free Radical Reactions in Living Mice" Biodence Mechanisms against Environmental Stress. (inpress).

H.Utsumi 等人：“活体小鼠自由基反应的体内 ESR 测量”针对环境压力的 Biodence 机制。

Tomokazu Yoshida, Hiroshi Otake, Yukihiro Aramaki, Toshifumi Hata, Seishi Tsuchiya, Akira Hamada, and Hideo Utsumi: "Free radicals from 1-palmitoyl-2-arachidonoyl phosphatidylcholine liposomes in Fe2+/ascorbic acid solution" Biol.Pharm.Bull.19(16). 779-78

Tomokazu Yoshida、Hiroshi Otake、Yukihiro Aramaki、Toshifumi Hata、Seishi Tsuchiya、Akira Hamada 和 Hideo Utsumi：“Fe2/抗坏血酸溶液中 1-棕榈酰-2-花生四烯酰磷脂酰胆碱脂质体中的自由基”Biol.Pharm.Bull.19(

韓 眞伊,竹下啓蔵,内海英雄: "生息マウス肺内のラジカル反応の無侵襲測定" 磁気共鳴と医学. 8. 47-50 (1997)

Mayi Han、Keizo Takeshita、Hideo Utsumi：“活体小鼠肺部自由基反应的非侵入性测量”《磁共振与医学》8. 47-50 (1997)。

K-I.Matsumoto,A.Hamada,and H.Utsumi: "Simultaneous ESR Imaging of spin Probes Encapsulated into and Released from Liposomes" Mag.Res.Med.6. 71-73 (1995)

K-I.Matsumoto、A.Hamada 和 H.Utsumi：“封装到脂质体中并从脂质体中释放的自旋探针的同步 ESR 成像”Mag.Res.Med.6。

韓 眞伊, 竹下 啓蔵, 内海 英雄: "生息マウス肺内のラジカル反応の無侵襲測定" 磁気共鳴と医学. 8. 47-50 (1997)

Mayi Han、Keizo Takeshita、Hideo Utsumi：“活体小鼠肺部自由基反应的非侵入性测量”《磁共振与医学》8. 47-50 (1997)。