Development of in vivo ESR for detecting free radicals in human living body

开发体内ESR检测人体自由基

• 批准号: 06557123
• 负责人: UTSUMI Hideo
• 金额: $ 9.66万
• 依托单位: Kyushu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-06557123/
• 关键词: Electron Spin Resanance; Free Radical; Active Oxygen; in vivo; ESR-CT; ラット; 関節炎; 糖尿病; ニトロキシドラジカル; 診断機器; SOD; 遺伝子組換えマウス; ESR; 活性酵素; 画像解析; イメージング; 生体レドックス; 一酸化窒素; 電子スビン共鳴(ESR); 一酸化窒素(NO)

ESR is a unique method to detect free radical reactions. The application of ESR was limited to small animals such as mice, however. In this study, 300 MHz ESR apparatus was developed, which is designed to measure in vivo free radical reactions in arm of human. Magnet, resonator and microwave unit were redesigned to measure large animals. This mewly developed ESR apparatus was manufactured and the following research was carried out with this new ESR apparatus.1.In rat adjuvant arthritis model, free radical reactions increased in adjuvant treated group than control group. The increment was correlated with the degree of oedema and was inhibited by treatments of Cu, Zn-SOD.Imaging of ESR-CT demonstrated that free radical reactions increased inflamed part of rat adjuvant arthritis.2.In rat diabetes model induced by streptzotocin, free radical reactions in upper abdomen increased compared with controlgroup, and this increment was inhibited by Cu, Zn-SOD treatment.3.This medium-sized ESR apparatus enabled to detect free radicals attached on arm of human, indicating that free radical reactions in human can be monitored with newly developed 300 MHz ESR apparatus.

ESR是检测自由基反应的独特方法。然而，ESR的应用仅限于小鼠等小动物。本研究研制了一种300 MHz ESR装置，用于测量人体手臂的自由基反应。重新设计了磁铁、谐振器和微波单元来测量大型动物。本研究采用自行研制的ESR仪进行了以下研究：1.佐剂性关节炎大鼠模型，佐剂治疗组自由基反应较对照组明显增强。2.在链脲佐菌素诱导的糖尿病大鼠模型中，与对照组相比，上腹部自由基反应增加，Cu，Zn-SOD可抑制这种增加，Zn-SOD治疗3.该装置能检测到人体手臂上的自由基，表明新研制的300 MHz ESR装置能监测人体的自由基反应。

项目成果

Yuri Miura,et al.: "In Vivo ESR Studies of Antioxidant Activity on Free Radical reaction in Living Mice under Oxidative Stress" Free Rad.Res.22(3). 209-214 (1995)

Yuri Miura 等人：“氧化应激下活小鼠自由基反应抗氧化活性的体内 ESR 研究”Free Rad.Res.22(3)。

Hideo Utsumi,et al.: "In vivo ESR measurements of free radical reactions in living animals" Curr.Topics Biophys. 20. 41-45 (1996)

Hideo Utsumi 等人：“活体动物自由基反应的体内 ESR 测量”Curr.Topics Biophys。

K-I.Matsumoto,A.Hamada,and H.Utsumi: "Simultaneous ESR Imaging of spin Probes Encapsulated into and Released from Liposomes" Mag.Res.Med.6. 71-73 (1995)

K-I.Matsumoto、A.Hamada 和 H.Utsumi：“封装到脂质体中并从脂质体中释放的自旋探针的同步 ESR 成像”Mag.Res.Med.6。

竹下啓蔵他: "マウス肺内ニトロキシルラジカル還元におけるSH化合物の役割 -L-バンドESRを用いたwhole body測定による検討" 磁気共鳴と医学. 7. 165-168 (1996)

Keizo Takeshita 等人：“SH 化合物在减少小鼠肺部硝酰基自由基方面的作用 - 使用 L 波段 ESR 进行全身测量的调查”《磁共振与医学》7. 165-168 (1996)。

Hideo Utsumi,et al.: "Bioradcals detected by ESR spectroscopy" H.Ohya-Nishikawa & L.Packer, 14 (1995)

Hideo Utsumi 等人：“通过 ESR 光谱法检测到的生物自由基” H.Ohya-Nishikawa