Development of multifactorial analysis system of adaptation behavior to various environments in freely-moving animals

自由活动动物适应各种环境行为的多因素分析系统的开发

• 批准号: 03557009
• 负责人: HORI Tetsuo
• 金额: $ 6.98万
• 依托单位: KYUSHU UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Developmental Scientific Research (B)
• 财政年份: 1991
• 资助国家: 日本
• 起止时间: 1991 至 1993
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-03557009/
• 关键词: Brain microdialysis; Noradrenaline; Serotonin; Immobilization Stress; Antidepressant; Splenic microdialysis; Interleukin-1 beta; Telemetry; 前頭前野; インターロイキン-1beta; 脳マイクロダイアリシス法; 脾臓マイクロダイアリシス法; テレメーター; ストレス; 不安誘起物質; 環境適応行動; テレメ-タ-; 脳内活性物質

We have developed long-term multifactorial analysis system of adaptation behaviors in freely moving animals. The details are as follows.1. Brain microdialysis system : This system consist of (a) high performance liquid chromatography, (b) electrochemical detector, (c) dialysis apparatus which collects extracellular fluid in the brain through dialysis membraine (made by hollow fibers, mol. wt. cutoff, 50kDa) and inject it into "(a)" using autoinjector.2. Measurement of brain noradrenaline (NA) and serotonin (5-HT) released from nerve terminals in freely moving rats : We analyzed changes in NA in the rat prefrontal cortex every 20 min using this system. Chromatograph peak, which had the same retension time as that of NA standard solution, increased by perfusion of methamphetamine, desipramine, and high K^+ solution, and decreased by that of Ca^<++> free, TTX containing and a-methyl-p-tyrosine containing solution. Furthermore, the peak was disappeared by dissection of the dorsal forebrain … More bundle. These findings indicate that the peak that we have detected corresponds to noradrenaline released from nerve terminals in the prefrontal cortex. Then we have examined the effects of immobilization stress. antidepressant and anxiogenic substances on NA release using this system. We could also detect changes in concentration of 5-HT in the prefrontal cortex.3. Splenic microdialysis : We were also successful in measuring concentration of NA in the spleen of freely moving rats. NA has been shown to be released from splenic sympathetic nerve terminals. We have found that splenic NA increases 6-7 times the baseline level by immobilization stress, and the increase is abolished by splenic denervation. Furthermore, splenic NA increased by systemic injection of interleukin-1 beta through an activation of central CRF system.4. Telemetry system : We have developed telemetry system by which body temperature of two different sites, locomotor activity, amount of food intake and drinking are measured in freely moving rats. Furthermore, we have examined the effects of interleukin-1 receptor antagonist on these parameters. Less

我们已经建立了自由活动动物适应行为的长期多因素分析系统。具体如下：1.脑微透析系统：该系统由高效液相色谱仪、电化学检测器、透析装置和生物传感器组成。重量截断值，50kDa），并使用自动注射器将其注射到"（a）"中。自由活动大鼠神经末梢释放的脑去甲肾上腺素（NA）和5-羟色胺（5-HT）的测量：我们使用该系统每20分钟分析大鼠前额叶皮层NA的变化。与NA标准溶液具有相同保留时间的色谱峰，在无Ca ^++、TTX和a-甲基-p-酪氨酸溶液中，随着甲基苯丙胺、地昔帕明和高K ^+溶液的灌注而增加，随着无Ca ^++、TTX和a-甲基-p-酪氨酸溶液的灌注而减少。此外，解剖背侧前脑后，该峰消失 ...更多信息 捆。这些发现表明，我们检测到的峰值对应于前额叶皮层神经末梢释放的去甲肾上腺素。然后，我们研究了固定压力的影响。抗抑郁药和焦虑物质对NA释放的影响。我们还可以检测到前额叶皮质5-HT浓度的变化。脾脏微透析：我们还成功地测量了自由活动大鼠脾脏中NA的浓度。NA已显示从脾交感神经末梢释放。我们已经发现，脾NA增加6 - 7倍的基线水平的制动应力，和增加被取消脾去神经。此外，通过激活中枢CRF系统，全身注射IL-1 β可增加脾脏NA.遥测系统：我们研制了一套遥测系统，用于测量自由活动大鼠的两个不同部位的体温、运动活动、摄食量和饮水量。此外，我们还研究了白细胞介素-1受体拮抗剂对这些参数的影响。少

项目成果

Niijima,A.: "The effects of interleukin-1β on the activity of adrenal,splenic and renal sympathetic nerves in the rat." Journal of Autonomic Nervous System. 36. 183-192 (1991)

Niijima, A.：“白细胞介素 1β 对大鼠肾上腺、脾和肾交感神经活动的影响。”自主神经系统杂志 36. 183-192 (1991)。

Shimizu,N.: "Hypothalamic microdialysis of mazindol causes anorexia with increase in synaptic serotonin in rats." Physiology and Behavior. 49. 131-134 (1991)

Shimizu,N.：“马吲哚的下丘脑微透析会导致大鼠厌食，并导致突触血清素增加。”

堀 哲郎: "ストレスと脳内活性物質の挙動." FRAGRANCE JOURNAL. 1991. 9-15 (1991)

Tetsuro Hori：“大脑中活性物质的压力和行为。” 香水杂志 1991. 9-15 (1991)

堀 哲郎: "視床下部・免疫系連関からみた発熱の中枢機序と生物学的意義." 内科宝函. 38. 202 (1991)

Tetsuro Hori：“从下丘脑-免疫系统关系的角度来看发烧的中心机制和生物学意义。” 38. 202 (1991)

Hori T.: "Capsaicin and central control of thermoregulation." Thermoregulation : Pathology, Pharmacology and Therapy, Pergamon Press, Inc. (New York). 619-648 (1991)

Hori T.：“辣椒素和体温调节的中枢控制。”