Flux of organic matter from phytoplankton to bacteria

有机物从浮游植物到细菌的通量

基本信息

批准号：
03640552
负责人：
HAMA Takeo
金额：
$ 1.54万
依托单位：
Nagoya University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1991
资助国家：
日本
起止时间：
1991 至 1992
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-03640552/
关键词：
Phytoplankton Bacteria Microbial loop Excretion Fatty acids ^<13>C-GC-MS method 溶存態有機物微生物ル-プ

项目摘要

1.Estimation of ^<13>C isotopic ratio of fatty acid by gas chromatography-mass spectrometry(GC-MS): The accuracy of the estimation of ^<13>C isotopic ratio of fatty acid was ascertained by using ^<13>C enriched palmitic acid. The results indicated the applicability of ^<13>C-GC-MS method to estimate the carbon flow from phytoplankton to bacteria.2.Production of fatty acid at subarctic and subtropical area of Northern Pacific: ^<13>C tracer experiments were carried out at subarctic and subtropical area of Northern Pacific. Fatty acid composition of photosynthetic products of phytoplankton was determined by ^<13>C-GC-MS method. Polyunsaturated fatty acids constituted major part of products at subarctic area, whereas saturated fatty acids were main product at subtropical region. This defference was mainly due to the difference in the water temperature of these region. High contribution of polyunsaturated fatty acids at subarctic region can keep the fluid condition of membrane of phytoplankton.3.Carbon flux to the bacterial specific fatty acids: The ^<13>C atomic ratio of bacterial specific fatty acids were estimated by GC-MS to elucidate carbon flow from phytoplankton to bacteria. Samples obtained from subarctic and subtropical region were analyzed. The ^<13>C atom % of bacterial specific fatty acids were much lower than phytoplankton fatty acids, indication that carbon flow form phytoplankton to bacteria was not high which has been suggested by ^<14>C tracer studies. However, the effects of same factors such as incubation length and ^<13>C atom% of phytoplankton carbon must be considered to estimate carbon flux.

1.通过使用 ^<13> c的同位素比率估计 ^<13> c的脂肪酸同位素比脂肪酸的同位素比：估计 ^<13> c同位素比脂肪酸的准确性是通过使用 ^<13> c富集的棕榈酸确定的。结果表明， ^<13> c-GC-MS方法的适用性估算了北太平洋北极地区亚热带和亚热带地区的脂肪酸的生产： ^<13> c tracer实验在北部太平洋北部的阳离子和亚热带地区进行。浮游植物的光合产物的脂肪酸组成由 ^<13> c-gc-ms方法确定。多不饱和脂肪酸在亚北极区域是产物的主要部分，而饱和脂肪酸是亚热带地区的主要产物。这种脱缘主要是由于这些区域的水温差异。多不饱和脂肪酸在亚北极区域的高贡献可以保持浮游植物的流体状况。3。碳通量对细菌特异性脂肪酸：细菌特异性脂肪酸的 ^<13> C原子比估算了GC-MS的估计，从而估算了来自Phytoplankston to Bactoria的GC-MS。分析了从亚北极和亚热带区域获得的样品。细菌特异性脂肪酸的 ^<13> c原子％远低于浮游植物脂肪酸，这表明碳流量形成浮游植物到细菌不高，这是由 ^<14> c tracer研究所建议的。但是，必须考虑相同因素（例如孵育长度和 ^<13> c原子％）的影响，以估计碳通量。