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Flux of organic matter from phytoplankton to bacteria

有机物从浮游植物到细菌的通量

基本信息

批准号：
03640552
负责人：
HAMA Takeo
金额：
$ 1.54万
依托单位：
Nagoya University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1991
资助国家：
日本
起止时间：
1991 至 1992
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-03640552/
关键词：
Phytoplankton Bacteria Microbial loop Excretion Fatty acids ^<13>C-GC-MS method 溶存態有機物微生物ル-プ

项目摘要

1.Estimation of ^<13>C isotopic ratio of fatty acid by gas chromatography-mass spectrometry(GC-MS): The accuracy of the estimation of ^<13>C isotopic ratio of fatty acid was ascertained by using ^<13>C enriched palmitic acid. The results indicated the applicability of ^<13>C-GC-MS method to estimate the carbon flow from phytoplankton to bacteria.2.Production of fatty acid at subarctic and subtropical area of Northern Pacific: ^<13>C tracer experiments were carried out at subarctic and subtropical area of Northern Pacific. Fatty acid composition of photosynthetic products of phytoplankton was determined by ^<13>C-GC-MS method. Polyunsaturated fatty acids constituted major part of products at subarctic area, whereas saturated fatty acids were main product at subtropical region. This defference was mainly due to the difference in the water temperature of these region. High contribution of polyunsaturated fatty acids at subarctic region can keep the fluid condition of membrane of phytoplankton.3.Carbon flux to the bacterial specific fatty acids: The ^<13>C atomic ratio of bacterial specific fatty acids were estimated by GC-MS to elucidate carbon flow from phytoplankton to bacteria. Samples obtained from subarctic and subtropical region were analyzed. The ^<13>C atom % of bacterial specific fatty acids were much lower than phytoplankton fatty acids, indication that carbon flow form phytoplankton to bacteria was not high which has been suggested by ^<14>C tracer studies. However, the effects of same factors such as incubation length and ^<13>C atom% of phytoplankton carbon must be considered to estimate carbon flux.

1.气相色谱-质谱（GC-MS）法测定脂肪酸中^<13>C同位素比值：用富集^<13>C的棕榈酸确定脂肪酸中^<13>C同位素比值的准确性。结果表明，^<13>C-GC-MS方法可用于估算浮游植物向细菌的碳流量。北太平洋亚北极和亚热带地区脂肪酸的生产：在北太平洋亚北极和亚热带地区进行了^<13>C示踪试验。采用^<13 bb0 C-GC-MS法测定了浮游植物光合产物的脂肪酸组成。亚北极地区以多不饱和脂肪酸为主，亚热带地区以饱和脂肪酸为主。这种差异主要是由于这些地区的水温差异造成的。亚北极地区多不饱和脂肪酸的高贡献可以保持浮游植物膜的流体状态。细菌特异性脂肪酸的碳通量：采用气相色谱-质谱法测定了细菌特异性脂肪酸的碳原子比^<13>，以阐明浮游植物向细菌的碳流动。对亚北极和亚热带地区的样品进行了分析。细菌特异性脂肪酸的^<13>C原子百分比远低于浮游植物脂肪酸，表明浮游植物向细菌的碳流并不高，这与^<14>C示踪研究的结论一致。然而，在估算碳通量时，必须考虑孵育长度和浮游植物碳的^<13>C原子%等相同因素的影响。