Stabilization and Activation of Enzymes by Adsorptive Immobilization with Water-Soluble Polymers and Its Application to Enzyme Membrane Reactor

水溶性聚合物吸附固定化酶的稳定和活化及其在酶膜反应器中的应用

基本信息

批准号：
04555229
负责人：
TERAMOTO Masaaki
金额：
$ 3.26万
依托单位：
Kyoto Institute of Technology
依托单位国家：
日本
项目类别：
Grant-in-Aid for Developmental Scientific Research (B)
财政年份：
1992
资助国家：
日本
起止时间：
1992 至 1993
项目状态：
已结题

项目摘要

1. Stabilization of enzymes and accelerating effect of water-soluble polymers on enzymic reaction(1) By addition of water-soluble cationic polymers such as polyethyleneimine(PEI) and DEAE-dextran, thermal stability of glucose dehydrogenase (GDH) was remarkably increased. It was deduced that adsorption of polymers to GDH prevents unfolding of GDH by multi-point binding.(2) The rate of GDH-catalyzed reaction was enhanced by addition of PEI at low coenzyme(NAD()^+) concentration. The results of kinetic study suggested that the accelerating effect is due to PEI-mediated enrichment of coenzyme NAD(P)^+ near GDH interacting with PEI.(3) In the reduction of phenylpyruvic acid(PhP) by lactate degydrogenase(LDH)/GDH conjugate system, addition of PEI was very effective for increasing reaction rate.(4) The apparent equilibrium constant of alcohol dehydrogenase(ADH)-catalyzed reaction was increased by addition of PEI, resulting in improvement of the conversion of PhP in LDH/ADH-catalyzed reaction.2. Effect of water-soluble polymers on performance of enzyme membrane reactorIn the continuous reduction of PhP by the LDH/GDH conjugate enzyme system using a continuoustype hollow fiber enzyme reactor, addition of PEI was effective for increasing the conversion of PhP.3. Preparation of microporous charged membrane by plasma-graft polymerization for membrane enzyme reactor.Microporpus charged membranes were prepared by grafting acrylic acid to microporous polyethylene membranes, and the effect of preparation condition of the membrane properties was studied. Membranes with high charge density were successfully prepared.

1。通过添加水溶性阳离子聚合物（例如聚乙烯亚胺（PEI））和deae--脱乳，葡萄糖脱氢酶（GDH）的热稳定性，通过添加水溶性阳离子聚合物（PEI）（PEI）等水溶性聚合物（例如），水溶性聚合物对酶反应的稳定和加速作用。推断出聚合物对GDH的吸附可通过多点结合来防止GDH展开。（2）通过在低辅酶（NAD（）（）^+）浓度下添加PEI，通过添加PEI来增强GDH催化反应的速率。动力学研究的结果表明，加速作用是由于PEI介导的辅酶NAD（P）^+近乎GDH与PEI相互作用的辅酶（3）（3）苯基丙酮酸（PHP）通过乳酸脱甲酸酯（PHP）通过乳酸脱甲酸酯酶（LDH）/GDH conjugate System添加了pei的频繁的速度（4）的频率（4）是有效的。通过添加PEI增加了酒精脱氢酶（ADH）催化反应，从而改善了LDH/ADH催化反应中PHP的转化。2。水溶性聚合物对酶膜反应蛋白的性能的影响，使用连续型中空纤维反应器LDH/GDH偶联酶系统连续减少PHP，PEI的添加有效地增加了PHP.3的转化。通过将丙烯酸嫁接到微孔聚乙烯膜和膜特性的制备效果来制备通过等离子接种聚合制备膜酶反应器的微孔电荷膜。高电荷密度的膜成功制备。