EFFECT OF MAGNETIC FIELD ON GENE EXPRESSION AND NUTATION INDUCTIC

磁场对基因表达和章动感应的影响

• 批准号: 06680503
• 负责人: MIYAKOSHI Junji
• 金额: $ 1.41万
• 依托单位: KYOTO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06680503/
• 关键词: MAGNETIC FIELD; TISSUE CULTURE; GENE EXPRESSION; SIGNAL TRANSDUCTION; MUTATION; 極低周波変動磁場; 細胞増殖; 初期応答遺伝子

Exposure of PC12-VG cells to an extremely low frequency magnetic field (ELFMF) enhanced the beta-galactosidase gene expression stimulated by teatment of the cells with forskolin. The enhancing effect of the ELFMF was inhibited by treatment of the cells with a specific inhibitor of PKC,calphostin C,as well as with the Ca^<2+> entry blockers nifedipin and dantrolen. Enhancement appeared within the first hour of a 4h forskolin treatment when the ELFMF was given at different times during culture. We speculate that exposure of PC12-VG cells to an ELFMF during the early response to forskolin treatment affects cell signal transduction, resulting in enhanced gene expression.On the other hand, an exposure to ELFMF of 50 Hz and 400mT induced mutations in the hypoxanthine-guanine phosphoribosyl transferase gene of human melanoma MeWo cells. The mutation frequency was enhanced both by increasing the exposure period and the induced current intensity. Mutations induced by X-rays were enhanced by ELFMF exposure. No significant increase in mutation frequency occurred when DNA replication was inhibited during ELFMF exposure. DNA replication error is suspected to cause the mutations produced by ELFMF exposure.

用极低频磁场（ELFMF）处理PC 12-VG细胞，可增强forskolin处理细胞所刺激的β-半乳糖苷酶基因表达。用PKC特异性抑制剂calphostin C以及Ca^2+进入阻断剂硝苯地平和丹曲伦处理细胞，可抑制ELFMF的增强作用。当ELFMF在培养过程中的不同时间给予时，在4 h毛喉素处理的第一个小时内出现增强。我们推测PC 12-VG细胞在对毛喉素处理的早期反应中暴露于ELFMF影响细胞信号转导，导致基因表达增强，另一方面，暴露于50 Hz和400 mT的ELFMF诱导人黑素瘤MeWo细胞次黄嘌呤-鸟嘌呤磷酸核糖转移酶基因突变。突变频率随暴露时间和诱导电流强度的增加而增加。ELFMF暴露增强了X射线诱导的突变。在ELFMF暴露期间，当DNA复制受到抑制时，突变频率没有显著增加。怀疑DNA复制错误导致ELFMF暴露产生的突变。

项目成果

Junji Miyakoshi, Shuji Ohtsu, Junko Tatsumi-Miyajima and Hiraku Takebe: "A newly designed experimental system for exposure of mammalian cells to extremely low frequency magnetic fields." Journal of Radiation Research. 35. 26-34 (1994)

Junji Miyakoshi、Shuji Ohtsu、Junko Tatsumi-Miyajima 和 Hiraku Takebe：“一种新设计的实验系统，用于将哺乳动物细胞暴露于极低频磁场中。”

Junji Miyakoshi: "A newly designed experimental. system for exposure of mammalian cells to extremly low freguency magnetic fields" Journal of Radiation Research. 35. 26-34 (1994)

Junji Miyakoshi：“一种新设计的实验系统，用于将哺乳动物细胞暴露于极低频磁场”辐射研究杂志。

Shuji Ohtsu: "Enhancement of β-galactosidase gene expression in rat pheoehromocytoma cells by exposure to extremely low frequency magnetic fields." Biochemical and Biophysical Research Communications. 212. 104-109 (1995)

Shuji Ohtsu：“通过暴露于极低频磁场增强大鼠嗜铬细胞瘤细胞中的 β-半乳糖苷酶基因表达。”生物化学和生物物理研究通讯。 212. 104-109 (1995)

Junji Miyakoshi: "Changes in radiation sensitivity of human osteosarcoma cells after p53 introduction." Japanese Journal of Cancer Research. 86. 711-713 (1995)

Junji Miyakoshi：“引入 p53 后人类骨肉瘤细胞的辐射敏感性发生变化。”

Junji Miyakoshi, Shuji Ohtsu, Masahiro Hiraoka, Mitsuyuki Abe and Hiraku Takabe.: "Exposure to 50 Hz magnetic field enhances beta-galactosidase activity in rat PC12-VG cells." Journal of Japanese Society of Applied Electromagnetic and Mechanics. 3. 52-56

Junji Miyakoshi、Shuji Ohtsu、Masahiro Hiraoka、Mitsuyuki Abe 和 Hiraku Takabe：“暴露于 50 Hz 磁场可增强大鼠 PC12-VG 细胞中的 β-半乳糖苷酶活性。”