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Cellular and molecular responses to power-frequency electromagnetic fields.

细胞和分子对工频电磁场的响应。

基本信息

批准号：
12831003
负责人：
MIYAKOSHI Junji
金额：
$ 2.3万
依托单位：
KYOTO UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2001
项目状态：
已结题

项目摘要

We investigated the change in gene expression of the stress protein, i.e., the heat shock protein in this study, under a high intensity of extremely low frequency (ELF) magnet field, and as a positive control, we investigated the effect of heat processing. In addition, we also studied a modifying effect of the magnetic field on gene expression of the heat shock protein under treatment of combination of the magnetic field and heat processing.1. The study on the heat shock protein was performed using HSP70, a typical heat shock protein, which reacts to numerous stresses including heat shock and expresses transiently. Goodman et al. (1997) reported ESP70 induction by ELF magnetic field exposure, however, the data at the level of μT were obtained and negative counterarguments were published. In this study, we investigated whether the exposure to a high magnetic intensity of ELF magnet field produces an effect on induction of the heat shock protein or not using human leukemia-derived cells (HL60) that Goodman et al. used. No induction of HSP70 protein expression was observed 24 hours after exposure to the 50 mT ELF magnetic field.2. As a positive control, we investigated the effect of heat processing. In addition, we also studied a modifying effect of the magnetic field on gene expression of the heat shock protein under treatment of combination of the ELF magnetic field and heat processing. Under a mild heat shock (40 - 42 ℃), induction of HSP70 protein expression was observed in HL60 cells. After combined treatment of this heat processing and exposure to the 50 mT ELF magnetic field, the level of HSP70 protein expression was lower than that under treatment of heat shock alone, which indicated that the ELF magnetic field inhibited the HSP70 protein expression. These results suggested that a high intensity of ELF magnetic field exaggerated the negative feedback action against the HSP70 expression induced by the heat shock and inhibited the HSP70 expression.

我们研究了应激蛋白基因表达的变化，即，本研究中的热休克蛋白，在高强度的极低频（ELF）磁场下，并作为阳性对照，我们研究了热处理的效果。此外，我们还研究了磁场与热处理联合处理对热休克蛋白基因表达的修饰作用.热休克蛋白70是一种典型的热休克蛋白，它对包括热休克在内的多种应激反应并瞬时表达。Goodman等人（1997）报告了ELF磁场暴露的ESP 70感应，然而，获得了μT水平的数据，并发表了否定的反驳意见。在本研究中，我们研究了暴露于ELF磁场的高磁场强度是否对Goodman等人使用的人白血病衍生细胞（HL 60）的热休克蛋白的诱导产生影响。50 mT极低频磁场作用24 h后，未观察到HSP 70蛋白表达的诱导作用.作为阳性对照，我们研究了热处理的影响。此外，我们还研究了极低频磁场与热处理相结合对热休克蛋白基因表达的修饰作用。在轻度热休克（40 - 42 ℃）条件下，HL 60细胞可诱导HSP 70蛋白表达。热处理与50 mT极低频磁场联合处理后，HSP 70蛋白表达水平低于单独热休克处理，表明极低频磁场抑制了HSP 70蛋白的表达。这些结果表明，高强度的ELF磁场增强了热休克诱导的HSP 70表达的负反馈作用，抑制了HSP 70的表达。