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Manufacture of an apparatus for exposure to magnetic fields and biological effect of the magntic fields

暴露于磁场和磁场的生物效应的装置的制造

基本信息

批准号：
07559007
负责人：
MIYAKOSHI Junji
金额：
$ 9.41万
依托单位：
KYOTO UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (A)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1997
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07559007/
关键词：
Magnetic fields Cultured cells Cell growth Gene expression Signal transduction Mutation induction

项目摘要

We had manufactured an appatus for exposure of cultured cells to low density of low frequency magnetic fields. We disigned and manufactured equipments for long-term and low-density (0 to 9 mT) exposures of cultured cells to extremely low frequency magnetic fields (ELFMF), and examined the effects of ELFMF on cell growth, c-myc mRNA expression and the mutation frequency in Chinese hamster ovary (CHO) cells. The ELFMF equipment consists of a CO2 incubator with a built-in magnet generator using Helmholtz coils being 250 mm in inner diameter, 160 mm in distance and 128 turns, a slide regulator and a thermocontroller. No significant difference in the growth rate and the c-mycexpression of CHO cells was observed with 5 mT ELFMF exposure, sham-exposure and incubation in a conventional incubator. On the other hand, CHO-K1 cells were exposed to 5 mT ELFMF at 60 Hz with or without initial X-irradiation for up to 12 weeks, and mutation frequency in the hypoxanthine-guanine phosphoribosyl transferase (HPRT) gene was analyzed using the 6-thioguanine-resistant mutation assay. The long-term exposures for up to 12 weeks to 5mT ELFMF did not increase mutations of HPRT gene in CHO-K1 cells. However, the enhancement of X-ray-induced mutations was observed in the treatment of X-irradiation followed by long-term 5 mT ELFMF exposures. The density of environmental ELFMF from high voltage power-lines should be at least several hundred times less than the density used in this study. Unless there are unknown mechanisms that involve mutagenesis under exposure to very highdensity ELFMF,cancer induction due to environmental ELFMF may be very low or unlikely. However, the effects of the combined exposure to X-rays abd ELFMF should be further investigated.

我们研制了一种用于低密度低频磁场作用下培养细胞的装置。设计并制作了极低频磁场（ELFMF）长期低密度（0~9mT）作用于培养细胞的装置，研究了ELFMF对中国仓鼠卵巢（CHO）细胞生长、c-myc mRNA表达和突变频率的影响。ELFMF设备由一个CO2培养箱、一个滑动调节器和一个温度控制器组成，该培养箱带有一个内置磁铁发生器，该磁铁发生器使用内径为250 mm、距离为160 mm、匝数为128圈的亥姆霍兹线圈。5 mT ELFMF暴露、假暴露和常规培养箱中孵育，CHO细胞的生长速率和c-myc表达无显着差异。另一方面，CHO-K1细胞暴露于5mT ELFMF，60 Hz，有或没有初始X射线照射长达12周，并在次黄嘌呤-鸟嘌呤磷酸核糖转移酶（HPRT）基因的突变频率进行了分析，使用6-硫代鸟嘌呤抗性突变试验。长期暴露于5mT ELFMF达12周并不增加CHO-K1细胞HPRT基因的突变。然而，X射线诱导的突变的增强，观察到在治疗的X射线照射，然后长期5 mT ELFMF曝光。来自高压电力线的环境ELFMF的密度应至少比本研究中使用的密度小几百倍。除非有未知的机制，涉及诱变下暴露于非常高密度的ELFMF，癌症诱导由于环境ELFMF可能是非常低或不太可能。然而，联合暴露于X射线和ELFMF的影响应进一步研究。